MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot study
Periodontitis is a chronic inflammatory disease that affects the interface of teeth and surrounding tissues. Gingival crevicular fluid (GCF) is an exudate of the periodontal tissues and can be collected from the gap between the tooth and gum (gingival sulcus or periodontal pocket) with paper strips....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-07-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12238 |
| _version_ | 1798018889500590080 |
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| author | Akira Saito Masafumi Horie Kenichiro Ejiri Akira Aoki Sayaka Katagiri Shogo Maekawa Shinta Suzuki Sophannary Kong Tsuneto Yamauchi Yoko Yamaguchi Yuichi Izumi Mitsuhiro Ohshima |
| author_facet | Akira Saito Masafumi Horie Kenichiro Ejiri Akira Aoki Sayaka Katagiri Shogo Maekawa Shinta Suzuki Sophannary Kong Tsuneto Yamauchi Yoko Yamaguchi Yuichi Izumi Mitsuhiro Ohshima |
| author_sort | Akira Saito |
| collection | DOAJ |
| description | Periodontitis is a chronic inflammatory disease that affects the interface of teeth and surrounding tissues. Gingival crevicular fluid (GCF) is an exudate of the periodontal tissues and can be collected from the gap between the tooth and gum (gingival sulcus or periodontal pocket) with paper strips. Testing of GCF is a low‐cost and minimally invasive procedure. In a variety of diseases, microRNAs (miRNAs) in body fluids are implicated in pathogenesis, and are suggested as potential diagnostic biomarkers. Here, we profiled miRNAs in GCF (two chronic periodontitis, one aggressive periodontitis, and five healthy subjects) using miRCURY LNA™ Universal RT microRNA PCR System, which yielded quantitative measures of more than 600 miRNAs. Through this analysis, we found that miRNA profiles in GCF of periodontitis patients are distinct from those of healthy controls. We further selected 40 miRNAs and confirmed their differential expression patterns in different subjects (five chronic periodontitis, one aggressive periodontitis, and six healthy subjects) using a custom miRNA PCR panel. This is the first demonstration of miRNA profiling in GCF and its alteration in periodontitis. Our findings suggest that a subset of miRNAs in GCF holds potential as a biomarker for periodontitis. |
| first_indexed | 2024-04-11T16:31:38Z |
| format | Article |
| id | doaj.art-98c01ceb48ae4a64b16bc774a254c306 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T16:31:38Z |
| publishDate | 2017-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-98c01ceb48ae4a64b16bc774a254c3062022-12-22T04:14:01ZengWileyFEBS Open Bio2211-54632017-07-017798199410.1002/2211-5463.12238MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot studyAkira Saito0Masafumi Horie1Kenichiro Ejiri2Akira Aoki3Sayaka Katagiri4Shogo Maekawa5Shinta Suzuki6Sophannary Kong7Tsuneto Yamauchi8Yoko Yamaguchi9Yuichi Izumi10Mitsuhiro Ohshima11Department of Respiratory Medicine Graduate School of Medicine The University of Tokyo JapanDepartment of Respiratory Medicine Graduate School of Medicine The University of Tokyo JapanDepartment of Periodontology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) JapanDepartment of Periodontology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) JapanDepartment of Periodontology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) JapanDepartment of Periodontology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) JapanDepartment of Periodontology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) JapanDepartment of Periodontology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) JapanDepartment of Mathematics Keio University Yokohama Kanagawa JapanDepartment of Biochemistry Nihon University School of Dentistry Tokyo JapanDepartment of Periodontology Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) JapanDepartment of Biochemistry Ohu University School of Pharmaceutical Sciences Koriyama Fukushima JapanPeriodontitis is a chronic inflammatory disease that affects the interface of teeth and surrounding tissues. Gingival crevicular fluid (GCF) is an exudate of the periodontal tissues and can be collected from the gap between the tooth and gum (gingival sulcus or periodontal pocket) with paper strips. Testing of GCF is a low‐cost and minimally invasive procedure. In a variety of diseases, microRNAs (miRNAs) in body fluids are implicated in pathogenesis, and are suggested as potential diagnostic biomarkers. Here, we profiled miRNAs in GCF (two chronic periodontitis, one aggressive periodontitis, and five healthy subjects) using miRCURY LNA™ Universal RT microRNA PCR System, which yielded quantitative measures of more than 600 miRNAs. Through this analysis, we found that miRNA profiles in GCF of periodontitis patients are distinct from those of healthy controls. We further selected 40 miRNAs and confirmed their differential expression patterns in different subjects (five chronic periodontitis, one aggressive periodontitis, and six healthy subjects) using a custom miRNA PCR panel. This is the first demonstration of miRNA profiling in GCF and its alteration in periodontitis. Our findings suggest that a subset of miRNAs in GCF holds potential as a biomarker for periodontitis.https://doi.org/10.1002/2211-5463.12238gingival crevicular fluidmicroRNAperiodontal pocketperiodontitis |
| spellingShingle | Akira Saito Masafumi Horie Kenichiro Ejiri Akira Aoki Sayaka Katagiri Shogo Maekawa Shinta Suzuki Sophannary Kong Tsuneto Yamauchi Yoko Yamaguchi Yuichi Izumi Mitsuhiro Ohshima MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot study FEBS Open Bio gingival crevicular fluid microRNA periodontal pocket periodontitis |
| title | MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot study |
| title_full | MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot study |
| title_fullStr | MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot study |
| title_full_unstemmed | MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot study |
| title_short | MicroRNA profiling in gingival crevicular fluid of periodontitis—a pilot study |
| title_sort | microrna profiling in gingival crevicular fluid of periodontitis a pilot study |
| topic | gingival crevicular fluid microRNA periodontal pocket periodontitis |
| url | https://doi.org/10.1002/2211-5463.12238 |
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