Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae)
We aimed to explore the entomopathogenic fungi (EPF) from the different ecosystems including forests, gardens, fields, and rangeland soils in Kermanshah province, Iran. Trials were carried out on morphological, molecular characterization, diversity indices, and virulence assessments of indigenous EP...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Entomological Society of Iran
2022-05-01
|
| Series: | نامه انجمن حشرهشناسی ایران |
| Subjects: | |
| Online Access: | https://jesi.areeo.ac.ir/article_127231_6825726d49f19da6203019a64789693f.pdf |
| _version_ | 1828083508921237504 |
|---|---|
| author | Hadi Mehrmoradi Samad Jamali Hamid Reza Pourian |
| author_facet | Hadi Mehrmoradi Samad Jamali Hamid Reza Pourian |
| author_sort | Hadi Mehrmoradi |
| collection | DOAJ |
| description | We aimed to explore the entomopathogenic fungi (EPF) from the different ecosystems including forests, gardens, fields, and rangeland soils in Kermanshah province, Iran. Trials were carried out on morphological, molecular characterization, diversity indices, and virulence assessments of indigenous EPF from 41 sampling sites of various localities. Using the Ephestia kuehniella (Zeller) as host bait, 114 fungal isolates were recovered-i.e., 39 from forests, 38 from fields, 22 from rangelands, and 15 from garden soils. Based on morphological features and the sequence analysis of the internal transcribed spacer (ITS) of the ribosomal DNA, the recovered entomopathogenic fungi were identified as Alternaria chlamydosporigena, Aspergillus nomius, Beauveria bassiana, B. pseudobassiana, B. brongniartii, Chaetomium elatum, Fusarium equiseti, F. oxysporum, Fusarium sp., Meyerozyma guilliermondii, Paramyrothecium roridum, Penicillium sizovae, P. solitum and Penicillium sp.. Higher species richness was found in the oak forest soils compared with fields, gardens, and rangelands. Additionally, the oak forest soils had high values of diversity indices, i.e., Simpson Ds (0.97), Shannon (3.30), Equitability (0.69), and Fisher's alpha (25.8). The dominance index was higher in the rangelands compared with the others. Following the priliminary assays, the insecticidal activity of three selected EPF isolates, including Beauveria brongniartii, B. bassiana, and B. pseudobassiana was tested against adults of the cowpea beetle, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchinae) as host. Hypervirulent strains caused high mortality considered as promising effective biocontrol agents in insect pest management programs. |
| first_indexed | 2024-04-11T04:09:48Z |
| format | Article |
| id | doaj.art-98c39a469ace484782989ae03ec06225 |
| institution | Directory Open Access Journal |
| issn | 0259-9996 2783-3968 |
| language | English |
| last_indexed | 2024-04-11T04:09:48Z |
| publishDate | 2022-05-01 |
| publisher | Entomological Society of Iran |
| record_format | Article |
| series | نامه انجمن حشرهشناسی ایران |
| spelling | doaj.art-98c39a469ace484782989ae03ec062252023-01-01T10:00:45ZengEntomological Society of Iranنامه انجمن حشرهشناسی ایران0259-99962783-39682022-05-01421295310.52547/jesi.42.1.3127231Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae)Hadi Mehrmoradi0Samad Jamali1Hamid Reza Pourian2Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, IranDepartment of Plant Protection, College of Agriculture, Razi University, Kermanshah, IranDepartment of Plant Protection, College of Agriculture, Razi University, Kermanshah, IranWe aimed to explore the entomopathogenic fungi (EPF) from the different ecosystems including forests, gardens, fields, and rangeland soils in Kermanshah province, Iran. Trials were carried out on morphological, molecular characterization, diversity indices, and virulence assessments of indigenous EPF from 41 sampling sites of various localities. Using the Ephestia kuehniella (Zeller) as host bait, 114 fungal isolates were recovered-i.e., 39 from forests, 38 from fields, 22 from rangelands, and 15 from garden soils. Based on morphological features and the sequence analysis of the internal transcribed spacer (ITS) of the ribosomal DNA, the recovered entomopathogenic fungi were identified as Alternaria chlamydosporigena, Aspergillus nomius, Beauveria bassiana, B. pseudobassiana, B. brongniartii, Chaetomium elatum, Fusarium equiseti, F. oxysporum, Fusarium sp., Meyerozyma guilliermondii, Paramyrothecium roridum, Penicillium sizovae, P. solitum and Penicillium sp.. Higher species richness was found in the oak forest soils compared with fields, gardens, and rangelands. Additionally, the oak forest soils had high values of diversity indices, i.e., Simpson Ds (0.97), Shannon (3.30), Equitability (0.69), and Fisher's alpha (25.8). The dominance index was higher in the rangelands compared with the others. Following the priliminary assays, the insecticidal activity of three selected EPF isolates, including Beauveria brongniartii, B. bassiana, and B. pseudobassiana was tested against adults of the cowpea beetle, Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae: Bruchinae) as host. Hypervirulent strains caused high mortality considered as promising effective biocontrol agents in insect pest management programs.https://jesi.areeo.ac.ir/article_127231_6825726d49f19da6203019a64789693f.pdfmolecular identificationfungal biodiversitylocal entomopathogenic fungivirulenceiran |
| spellingShingle | Hadi Mehrmoradi Samad Jamali Hamid Reza Pourian Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae) نامه انجمن حشرهشناسی ایران molecular identification fungal biodiversity local entomopathogenic fungi virulence iran |
| title | Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae) |
| title_full | Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae) |
| title_fullStr | Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae) |
| title_full_unstemmed | Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae) |
| title_short | Isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against Callosobruchus maculatus (F.) (Coleoptera:Chrysomelidae) |
| title_sort | isolation of entomopathogenic fungi from cultivated and uncultivated soils and evaluation of virulence against callosobruchus maculatus f coleoptera chrysomelidae |
| topic | molecular identification fungal biodiversity local entomopathogenic fungi virulence iran |
| url | https://jesi.areeo.ac.ir/article_127231_6825726d49f19da6203019a64789693f.pdf |
| work_keys_str_mv | AT hadimehrmoradi isolationofentomopathogenicfungifromcultivatedanduncultivatedsoilsandevaluationofvirulenceagainstcallosobruchusmaculatusfcoleopterachrysomelidae AT samadjamali isolationofentomopathogenicfungifromcultivatedanduncultivatedsoilsandevaluationofvirulenceagainstcallosobruchusmaculatusfcoleopterachrysomelidae AT hamidrezapourian isolationofentomopathogenicfungifromcultivatedanduncultivatedsoilsandevaluationofvirulenceagainstcallosobruchusmaculatusfcoleopterachrysomelidae |