Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen Species
Inflammation and oxidative stress are closely related processes in the pathogenesis of various ocular diseases. Uveitis is a disorder of the uvea and ocular tissues that causes extreme pain, decreases visual acuity, and can eventually lead to blindness. The pharmacological functions of fucoxanthin,...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/7/1092 |
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| author | Shiu-Jau Chen Tzer-Bin Lin Hsien-Yu Peng Cheng-Hsien Lin An-Sheng Lee Hsiang-Jui Liu Chun-Chieh Li Kuang-Wen Tseng |
| author_facet | Shiu-Jau Chen Tzer-Bin Lin Hsien-Yu Peng Cheng-Hsien Lin An-Sheng Lee Hsiang-Jui Liu Chun-Chieh Li Kuang-Wen Tseng |
| author_sort | Shiu-Jau Chen |
| collection | DOAJ |
| description | Inflammation and oxidative stress are closely related processes in the pathogenesis of various ocular diseases. Uveitis is a disorder of the uvea and ocular tissues that causes extreme pain, decreases visual acuity, and can eventually lead to blindness. The pharmacological functions of fucoxanthin, isolated from brown algae, induce a variety of therapeutic effects such as oxidative stress reduction and repression of inflammation reactions. However, the specific anti-inflammatory effects of fucoxanthin on pathogen-associated molecular pattern (PAMP) lipopolysaccharide-induced uveitis have yet to be extensively described. Therefore, the aim of present study was to investigate the anti-inflammatory effects of fucoxanthin on uveitis in rats. The results showed that fucoxanthin effectively enhanced the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) in ocular tissues. Furthermore, fucoxanthin significantly increased the ocular activities of superoxide dismutase and decreased the levels of malondialdehyde stimulated by PAMP-induced uveitis. Ocular hypertension and the levels of inflammatory cells and proinflammatory cytokine tumor necrosis factor-alpha in the aqueous humor were alleviated with fucoxanthin treatment. Consequently, compared to the observed effects in lipopolysaccharide groups, fucoxanthin treatment significantly preserved iris sphincter innervation and pupillary function. Additionally, PAMP-induced corneal endothelial disruption was significantly inhibited by fucoxanthin treatment. Overall, these findings suggest that fucoxanthin may protect against inflammation from PAMP-induced uveitis by promoting the Nrf2 pathway and inhibiting oxidative stress. |
| first_indexed | 2024-03-10T09:47:06Z |
| format | Article |
| id | doaj.art-98c3cfc51c674e4298de49aa1d1d02ea |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T09:47:06Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-98c3cfc51c674e4298de49aa1d1d02ea2023-11-22T03:05:21ZengMDPI AGAntioxidants2076-39212021-07-01107109210.3390/antiox10071092Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen SpeciesShiu-Jau Chen0Tzer-Bin Lin1Hsien-Yu Peng2Cheng-Hsien Lin3An-Sheng Lee4Hsiang-Jui Liu5Chun-Chieh Li6Kuang-Wen Tseng7Department of Neurosurgery, Mackay Memorial Hospital, Taipei 10449, TaiwanDepartment of Physiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11049, TaiwanDepartment of Medicine, Mackay Medical College, New Taipei 25245, TaiwanDepartment of Medicine, Mackay Medical College, New Taipei 25245, TaiwanDepartment of Medicine, Mackay Medical College, New Taipei 25245, TaiwanDepartment of Optometry, MacKay Junior College of Medicine, Nursing, and Management, New Taipei 11260, TaiwanDepartment of Medicine, Mackay Medical College, New Taipei 25245, TaiwanDepartment of Medicine, Mackay Medical College, New Taipei 25245, TaiwanInflammation and oxidative stress are closely related processes in the pathogenesis of various ocular diseases. Uveitis is a disorder of the uvea and ocular tissues that causes extreme pain, decreases visual acuity, and can eventually lead to blindness. The pharmacological functions of fucoxanthin, isolated from brown algae, induce a variety of therapeutic effects such as oxidative stress reduction and repression of inflammation reactions. However, the specific anti-inflammatory effects of fucoxanthin on pathogen-associated molecular pattern (PAMP) lipopolysaccharide-induced uveitis have yet to be extensively described. Therefore, the aim of present study was to investigate the anti-inflammatory effects of fucoxanthin on uveitis in rats. The results showed that fucoxanthin effectively enhanced the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) in ocular tissues. Furthermore, fucoxanthin significantly increased the ocular activities of superoxide dismutase and decreased the levels of malondialdehyde stimulated by PAMP-induced uveitis. Ocular hypertension and the levels of inflammatory cells and proinflammatory cytokine tumor necrosis factor-alpha in the aqueous humor were alleviated with fucoxanthin treatment. Consequently, compared to the observed effects in lipopolysaccharide groups, fucoxanthin treatment significantly preserved iris sphincter innervation and pupillary function. Additionally, PAMP-induced corneal endothelial disruption was significantly inhibited by fucoxanthin treatment. Overall, these findings suggest that fucoxanthin may protect against inflammation from PAMP-induced uveitis by promoting the Nrf2 pathway and inhibiting oxidative stress.https://www.mdpi.com/2076-3921/10/7/1092fucoxanthinlipopolysaccharideuveitisoxidative stressnuclear factor erythroid 2-related factor 2 |
| spellingShingle | Shiu-Jau Chen Tzer-Bin Lin Hsien-Yu Peng Cheng-Hsien Lin An-Sheng Lee Hsiang-Jui Liu Chun-Chieh Li Kuang-Wen Tseng Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen Species Antioxidants fucoxanthin lipopolysaccharide uveitis oxidative stress nuclear factor erythroid 2-related factor 2 |
| title | Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen Species |
| title_full | Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen Species |
| title_fullStr | Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen Species |
| title_full_unstemmed | Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen Species |
| title_short | Protective Effects of Fucoxanthin Dampen Pathogen-Associated Molecular Pattern (PAMP) Lipopolysaccharide-Induced Inflammatory Action and Elevated Intraocular Pressure by Activating Nrf2 Signaling and Generating Reactive Oxygen Species |
| title_sort | protective effects of fucoxanthin dampen pathogen associated molecular pattern pamp lipopolysaccharide induced inflammatory action and elevated intraocular pressure by activating nrf2 signaling and generating reactive oxygen species |
| topic | fucoxanthin lipopolysaccharide uveitis oxidative stress nuclear factor erythroid 2-related factor 2 |
| url | https://www.mdpi.com/2076-3921/10/7/1092 |
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