Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells.
Berberine is a primary component of the most functional extracts of Coptidis rhizome used in traditional Chinese medicine for centuries. Recent reports indicate that Berberine has the potential to prevent and treat Alzheimer's disease (AD). The previous studies reported that Calyculin A (CA) im...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3979860?pdf=render |
| _version_ | 1818519750697811968 |
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| author | Xiaofeng Liu Jie Zhou Morad Dirhem Naji Abid Huanhuan Yan Hao Huang Limin Wan Zuohua Feng Juan Chen |
| author_facet | Xiaofeng Liu Jie Zhou Morad Dirhem Naji Abid Huanhuan Yan Hao Huang Limin Wan Zuohua Feng Juan Chen |
| author_sort | Xiaofeng Liu |
| collection | DOAJ |
| description | Berberine is a primary component of the most functional extracts of Coptidis rhizome used in traditional Chinese medicine for centuries. Recent reports indicate that Berberine has the potential to prevent and treat Alzheimer's disease (AD). The previous studies reported that Calyculin A (CA) impaired the axonal transport in neuroblastoma-2a (N2a) cells. Berberine attenuated tau hyperphosphorylation and cytotoxicity induced by CA. Our study aimed at investigating the effects of Berberine on the axonal transport impairment induced by CA in N2a cells. The results showed that Berberine could protect the cell from CA -induced toxicity in metabolism and viability, as well as hyperphosphorylation of tau and neurofilaments (NFs). Furthermore, Berberine could reverse CA-induced axonal transport impairment significantly. Berberine also partially reversed the phosphorylation of the catalytic subunit of PP-2A at Tyrosine 307, a crucial site negatively regulating the activity of PP-2A, and reduced the levels of malondialdehyde and the activity of superoxide dismutase, markers of oxidative stress, induced by CA. The present work for the first time demonstrates that Berberine may play a role in protecting against CA-induced axonal transport impairment by modulating the activity of PP-2A and oxidative stress. Our findings also suggest that Berberine may be a potential therapeutic drug for AD. |
| first_indexed | 2024-12-11T01:28:14Z |
| format | Article |
| id | doaj.art-98c6d5f840a24c45a895a7e505518888 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T01:28:14Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-98c6d5f840a24c45a895a7e5055188882022-12-22T01:25:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9397410.1371/journal.pone.0093974Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells.Xiaofeng LiuJie ZhouMorad Dirhem Naji AbidHuanhuan YanHao HuangLimin WanZuohua FengJuan ChenBerberine is a primary component of the most functional extracts of Coptidis rhizome used in traditional Chinese medicine for centuries. Recent reports indicate that Berberine has the potential to prevent and treat Alzheimer's disease (AD). The previous studies reported that Calyculin A (CA) impaired the axonal transport in neuroblastoma-2a (N2a) cells. Berberine attenuated tau hyperphosphorylation and cytotoxicity induced by CA. Our study aimed at investigating the effects of Berberine on the axonal transport impairment induced by CA in N2a cells. The results showed that Berberine could protect the cell from CA -induced toxicity in metabolism and viability, as well as hyperphosphorylation of tau and neurofilaments (NFs). Furthermore, Berberine could reverse CA-induced axonal transport impairment significantly. Berberine also partially reversed the phosphorylation of the catalytic subunit of PP-2A at Tyrosine 307, a crucial site negatively regulating the activity of PP-2A, and reduced the levels of malondialdehyde and the activity of superoxide dismutase, markers of oxidative stress, induced by CA. The present work for the first time demonstrates that Berberine may play a role in protecting against CA-induced axonal transport impairment by modulating the activity of PP-2A and oxidative stress. Our findings also suggest that Berberine may be a potential therapeutic drug for AD.http://europepmc.org/articles/PMC3979860?pdf=render |
| spellingShingle | Xiaofeng Liu Jie Zhou Morad Dirhem Naji Abid Huanhuan Yan Hao Huang Limin Wan Zuohua Feng Juan Chen Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells. PLoS ONE |
| title | Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells. |
| title_full | Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells. |
| title_fullStr | Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells. |
| title_full_unstemmed | Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells. |
| title_short | Berberine attenuates axonal transport impairment and axonopathy induced by Calyculin A in N2a cells. |
| title_sort | berberine attenuates axonal transport impairment and axonopathy induced by calyculin a in n2a cells |
| url | http://europepmc.org/articles/PMC3979860?pdf=render |
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