Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea Crops
Fertile Chernozems of Southern Russia are of great value, so it is important to study the impact of agricultural activities on the soil quality. Changes in taxonomic composition and α-diversity of microbial communities of agricultural soils occupied by pea (<i>Pisum sativum</i> L.) and c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/3/750 |
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| author | Ludmila Khmelevtsova Elizaveta Konstantinova Shorena Karchava Maria Klimova Tatiana Azhogina Elena Polienko Margarita Khammami Ivan Sazykin Marina Sazykina |
| author_facet | Ludmila Khmelevtsova Elizaveta Konstantinova Shorena Karchava Maria Klimova Tatiana Azhogina Elena Polienko Margarita Khammami Ivan Sazykin Marina Sazykina |
| author_sort | Ludmila Khmelevtsova |
| collection | DOAJ |
| description | Fertile Chernozems of Southern Russia are of great value, so it is important to study the impact of agricultural activities on the soil quality. Changes in taxonomic composition and α-diversity of microbial communities of agricultural soils occupied by pea (<i>Pisum sativum</i> L.) and chickpea (<i>Cicer arietinum</i> L.) in response of cropland management were studied. A field experiment was conducted under four different conditions: (1) control, (2) mineral fertilizers (NPK) application alone, (3) pesticides application alone, and (4) fertilization combined with pesticides. The taxonomic composition of the soil bacterial community was studied by amplification and sequencing of the 16S rRNA gene. The predominance of Actinobacteria (17.7–32.3%), Proteobacteria (17.7–28.2%), Planctomycetes (10.1–21.3%), Acidobacteria (5.3–11.1%), Chloroflexi (1.0–7.1%), Gemmatimonadetes (2.5–8.0%), Bacteroidetes (3.6–11.3%), and Verrucomicrobia (3.9–9.2%) was noted. Introduction of pesticides led to an increase in the relative abundance of Chlorobi and Gemmatimonadetes. The time of sampling was the main significant factor determining the differences in the structure of soil microbial communities. All treatments did not have a significant effect on the α-diversity of the studied soils. Thus, treatment with mineral fertilizers and pesticides does not have a significant negative effect on the bacterial community of cultivated soils. |
| first_indexed | 2024-03-11T07:03:39Z |
| format | Article |
| id | doaj.art-98c70447b524406a8eaabc107c829be7 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T07:03:39Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-98c70447b524406a8eaabc107c829be72023-11-17T09:05:39ZengMDPI AGAgronomy2073-43952023-03-0113375010.3390/agronomy13030750Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea CropsLudmila Khmelevtsova0Elizaveta Konstantinova1Shorena Karchava2Maria Klimova3Tatiana Azhogina4Elena Polienko5Margarita Khammami6Ivan Sazykin7Marina Sazykina8Academy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaAcademy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaAcademy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaAcademy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaAcademy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaFederal State Budget Scientific Institution “Federal Rostov Agricultural Research Centre”, Rassvet, 346735 Rostov Oblast, RussiaAcademy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaAcademy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaAcademy of Biology and Biotechnologies, Southern Federal University, 344090 Rostov-on-Don, RussiaFertile Chernozems of Southern Russia are of great value, so it is important to study the impact of agricultural activities on the soil quality. Changes in taxonomic composition and α-diversity of microbial communities of agricultural soils occupied by pea (<i>Pisum sativum</i> L.) and chickpea (<i>Cicer arietinum</i> L.) in response of cropland management were studied. A field experiment was conducted under four different conditions: (1) control, (2) mineral fertilizers (NPK) application alone, (3) pesticides application alone, and (4) fertilization combined with pesticides. The taxonomic composition of the soil bacterial community was studied by amplification and sequencing of the 16S rRNA gene. The predominance of Actinobacteria (17.7–32.3%), Proteobacteria (17.7–28.2%), Planctomycetes (10.1–21.3%), Acidobacteria (5.3–11.1%), Chloroflexi (1.0–7.1%), Gemmatimonadetes (2.5–8.0%), Bacteroidetes (3.6–11.3%), and Verrucomicrobia (3.9–9.2%) was noted. Introduction of pesticides led to an increase in the relative abundance of Chlorobi and Gemmatimonadetes. The time of sampling was the main significant factor determining the differences in the structure of soil microbial communities. All treatments did not have a significant effect on the α-diversity of the studied soils. Thus, treatment with mineral fertilizers and pesticides does not have a significant negative effect on the bacterial community of cultivated soils.https://www.mdpi.com/2073-4395/13/3/750Calcic Chernozemssoil microbiomemicrobial communitiessoil bacteriaalpha diversitycropland management |
| spellingShingle | Ludmila Khmelevtsova Elizaveta Konstantinova Shorena Karchava Maria Klimova Tatiana Azhogina Elena Polienko Margarita Khammami Ivan Sazykin Marina Sazykina Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea Crops Agronomy Calcic Chernozems soil microbiome microbial communities soil bacteria alpha diversity cropland management |
| title | Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea Crops |
| title_full | Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea Crops |
| title_fullStr | Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea Crops |
| title_full_unstemmed | Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea Crops |
| title_short | Influence of Pesticides and Mineral Fertilizers on the Bacterial Community of Arable Soils under Pea and Chickpea Crops |
| title_sort | influence of pesticides and mineral fertilizers on the bacterial community of arable soils under pea and chickpea crops |
| topic | Calcic Chernozems soil microbiome microbial communities soil bacteria alpha diversity cropland management |
| url | https://www.mdpi.com/2073-4395/13/3/750 |
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