Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats
Circadian rhythm disturbances caused by anaesthesia may be one of the important reasons of many disturbances following surgery. The effects of 6% desflurane on suprachiasmatic nucleus (SCN), liver, and pancreas PER2, CRY1, BMAL, and CLOCK circadian protein levels and serum concentrations of glucose,...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2024-02-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3087 |
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| author | Ilknur Hatice AKBUDAK Ozgen KILIC-ERKEK Melek BOR-KUCUKATAY |
| author_facet | Ilknur Hatice AKBUDAK Ozgen KILIC-ERKEK Melek BOR-KUCUKATAY |
| author_sort | Ilknur Hatice AKBUDAK |
| collection | DOAJ |
| description | Circadian rhythm disturbances caused by anaesthesia may be one of the important
reasons of many disturbances following surgery. The effects of 6% desflurane on
suprachiasmatic nucleus (SCN), liver, and pancreas PER2, CRY1, BMAL, and CLOCK
circadian protein levels and serum concentrations of glucose, melatonin, cortisol 24 h
following the administration of anaesthesia were determined in this study. 10–12-weekold
Wistar albino male rats (n=12) were divided into two groups. Control group (C)
was not exposed to desflurane, while the anaesthesia group (D) received desflurane at
6% concentration for 6 h between 07.00 and 13.00 a.m. and was euthanized 24 h after
anaesthesia. Tissue samples were collected from both groups at the same time of the
day (07.00 a.m.). Serum glucose, melatonin, cortisol and SCN, liver, pancreas CLOCK,
BMAL1, PER2, CRY1 levels were determined using commercial kits. CLOCK and PER2
protein levels in SCN and BMAL1 protein concentration in liver decreased, while liver
PER2 protein level increased at the 24th h following anaesthesia. Pancreas BMAL1,
CLOCK, PER2, CRY1 protein levels were not affected by the anaesthesia protocol
applied. No statistically significant alteration was observed in plasma glucose, cortisol
and melatonin concentrations. Our findings show that, the circadian clock rhythm in
SCN and liver are still affected at the 24th h following exposure to desflurane. |
| first_indexed | 2024-03-08T04:10:40Z |
| format | Article |
| id | doaj.art-98c73521abc84335a1d50eca9b1ab176 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-08T04:10:40Z |
| publishDate | 2024-02-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-98c73521abc84335a1d50eca9b1ab1762024-02-08T20:14:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512024-02-0130217918410.9775/kvfd.2023.303963087Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in ratsIlknur Hatice AKBUDAK0Ozgen KILIC-ERKEK1Melek BOR-KUCUKATAY2Pamukkale University, Faculty of Medicine, Department of Anesthesiology and Reanimation, TR-20160 Kinikli, Denizli - TÜRKİYEPamukkale University, Faculty of Medicine, Department of Physiology, TR-20160 Kinikli, Denizli - TÜRKİYEPamukkale University, Faculty of Medicine, Department of Physiology, TR-20160 Kinikli, Denizli - TÜRKİYECircadian rhythm disturbances caused by anaesthesia may be one of the important reasons of many disturbances following surgery. The effects of 6% desflurane on suprachiasmatic nucleus (SCN), liver, and pancreas PER2, CRY1, BMAL, and CLOCK circadian protein levels and serum concentrations of glucose, melatonin, cortisol 24 h following the administration of anaesthesia were determined in this study. 10–12-weekold Wistar albino male rats (n=12) were divided into two groups. Control group (C) was not exposed to desflurane, while the anaesthesia group (D) received desflurane at 6% concentration for 6 h between 07.00 and 13.00 a.m. and was euthanized 24 h after anaesthesia. Tissue samples were collected from both groups at the same time of the day (07.00 a.m.). Serum glucose, melatonin, cortisol and SCN, liver, pancreas CLOCK, BMAL1, PER2, CRY1 levels were determined using commercial kits. CLOCK and PER2 protein levels in SCN and BMAL1 protein concentration in liver decreased, while liver PER2 protein level increased at the 24th h following anaesthesia. Pancreas BMAL1, CLOCK, PER2, CRY1 protein levels were not affected by the anaesthesia protocol applied. No statistically significant alteration was observed in plasma glucose, cortisol and melatonin concentrations. Our findings show that, the circadian clock rhythm in SCN and liver are still affected at the 24th h following exposure to desflurane.https://vetdergikafkas.org/pdf.php?id=3087circadian rhythmclock proteinsdesfluraneliverscn |
| spellingShingle | Ilknur Hatice AKBUDAK Ozgen KILIC-ERKEK Melek BOR-KUCUKATAY Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats Kafkas Universitesi Veteriner Fakültesi Dergisi circadian rhythm clock proteins desflurane liver scn |
| title | Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats |
| title_full | Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats |
| title_fullStr | Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats |
| title_full_unstemmed | Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats |
| title_short | Influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats |
| title_sort | influences of desflurane on the central and hepatic circadian clock persist at 24 hours following anaesthesia in rats |
| topic | circadian rhythm clock proteins desflurane liver scn |
| url | https://vetdergikafkas.org/pdf.php?id=3087 |
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