Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery Disease
Abstract Background: Oxidative stress and inflammation are present in coronary artery disease (CAD) and are linked to the activation of the transcription nuclear factor kappa B (NF-κB). To attenuate these complications, transcription factors like nuclear factor erythroid 2-related factor 2 (Nrf2) a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Cardiologia (SBC)
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| Series: | Arquivos Brasileiros de Cardiologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2019005001205&lng=en&tlng=en |
| _version_ | 1818858667705892864 |
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| author | Jaqueline Ermida Barbosa Milena Barcza Stockler-Pinto Beatriz Oliveira da Cruz Ana Carla Tavares da Silva Juliana Saraiva Anjos Claudio Tinoco Mesquita Denise Mafra Ludmila F. M. F. Cardozo |
| author_facet | Jaqueline Ermida Barbosa Milena Barcza Stockler-Pinto Beatriz Oliveira da Cruz Ana Carla Tavares da Silva Juliana Saraiva Anjos Claudio Tinoco Mesquita Denise Mafra Ludmila F. M. F. Cardozo |
| author_sort | Jaqueline Ermida Barbosa |
| collection | DOAJ |
| description | Abstract Background: Oxidative stress and inflammation are present in coronary artery disease (CAD) and are linked to the activation of the transcription nuclear factor kappa B (NF-κB). To attenuate these complications, transcription factors like nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) can be activated to inhibit NF-κB. However, the available data on expression of NF-κB, Nrf2 and PPARβ/δ in CAD patients are limited. Objective: To evaluate the expression of the transcription factors NF-κB and Nrf2 and PPAR��/�� in CAD patients. Methods: Thirty-five patients (17 men, mean age 62.4 ? 7.55 years) with CAD and twelve patients (5 men, mean age 63.50 ? 11.46 years) without CAD were enrolled. Peripheral blood mononuclear cells (PBMCs) were isolated and processed for mRNA expression of Nrf2, NF-κB, NADPH: quinone oxidoreductase 1 (NQO1) and PPARβ/δ mRNAs using quantitative real-time polymerase chain reaction (qPCR). p < 0.05 was considered statistically significant. Results: There was no difference in the mRNA expressions of Nrf2 (1.35 ? 0.57), NF-κB (1.08 ? 0.50) or in the antioxidant enzyme NQO1 (1.05 ? 0.88) in the CAD group compared to the group without CAD (1.16 ? 0.76, 0.95 ? 0.33, 0.81 ? 0.55, respectively). However, PPARβ/δ was highest expressed in the CAD group (1.17 ? 0.86 vs. 0.56 ? 0.34, p = 0.008). Conclusion: The main finding of this study was the PPARβ/δ being more expressed in the PBMC of patients with CAD compared to the control group, whereas no differences were observed in Nrf2 or NF-κB mRNA expressions. |
| first_indexed | 2024-12-19T08:59:56Z |
| format | Article |
| id | doaj.art-98c78fc80cfa4c8b8229ce6a73e47d5d |
| institution | Directory Open Access Journal |
| issn | 1678-4170 |
| language | English |
| last_indexed | 2024-12-19T08:59:56Z |
| publisher | Sociedade Brasileira de Cardiologia (SBC) |
| record_format | Article |
| series | Arquivos Brasileiros de Cardiologia |
| spelling | doaj.art-98c78fc80cfa4c8b8229ce6a73e47d5d2022-12-21T20:28:30ZengSociedade Brasileira de Cardiologia (SBC)Arquivos Brasileiros de Cardiologia1678-417010.5935/abc.20190125S0066-782X2019005001205Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery DiseaseJaqueline Ermida BarbosaMilena Barcza Stockler-PintoBeatriz Oliveira da CruzAna Carla Tavares da SilvaJuliana Saraiva AnjosClaudio Tinoco MesquitaDenise MafraLudmila F. M. F. CardozoAbstract Background: Oxidative stress and inflammation are present in coronary artery disease (CAD) and are linked to the activation of the transcription nuclear factor kappa B (NF-κB). To attenuate these complications, transcription factors like nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) can be activated to inhibit NF-κB. However, the available data on expression of NF-κB, Nrf2 and PPARβ/δ in CAD patients are limited. Objective: To evaluate the expression of the transcription factors NF-κB and Nrf2 and PPAR��/�� in CAD patients. Methods: Thirty-five patients (17 men, mean age 62.4 ? 7.55 years) with CAD and twelve patients (5 men, mean age 63.50 ? 11.46 years) without CAD were enrolled. Peripheral blood mononuclear cells (PBMCs) were isolated and processed for mRNA expression of Nrf2, NF-κB, NADPH: quinone oxidoreductase 1 (NQO1) and PPARβ/δ mRNAs using quantitative real-time polymerase chain reaction (qPCR). p < 0.05 was considered statistically significant. Results: There was no difference in the mRNA expressions of Nrf2 (1.35 ? 0.57), NF-κB (1.08 ? 0.50) or in the antioxidant enzyme NQO1 (1.05 ? 0.88) in the CAD group compared to the group without CAD (1.16 ? 0.76, 0.95 ? 0.33, 0.81 ? 0.55, respectively). However, PPARβ/δ was highest expressed in the CAD group (1.17 ? 0.86 vs. 0.56 ? 0.34, p = 0.008). Conclusion: The main finding of this study was the PPARβ/δ being more expressed in the PBMC of patients with CAD compared to the control group, whereas no differences were observed in Nrf2 or NF-κB mRNA expressions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2019005001205&lng=en&tlng=enCoronary Artery DiseaseOxidative StressInflammationObesityHypertensionDyslipidemiasRisk Factors/prevalenceMyocardial InfarctionHeart Failure |
| spellingShingle | Jaqueline Ermida Barbosa Milena Barcza Stockler-Pinto Beatriz Oliveira da Cruz Ana Carla Tavares da Silva Juliana Saraiva Anjos Claudio Tinoco Mesquita Denise Mafra Ludmila F. M. F. Cardozo Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery Disease Arquivos Brasileiros de Cardiologia Coronary Artery Disease Oxidative Stress Inflammation Obesity Hypertension Dyslipidemias Risk Factors/prevalence Myocardial Infarction Heart Failure |
| title | Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery Disease |
| title_full | Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery Disease |
| title_fullStr | Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery Disease |
| title_full_unstemmed | Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery Disease |
| title_short | Nrf2, NF-κB and PPARβ/δ mRNA Expression Profile in Patients with Coronary Artery Disease |
| title_sort | nrf2 nf κb and pparβ δ mrna expression profile in patients with coronary artery disease |
| topic | Coronary Artery Disease Oxidative Stress Inflammation Obesity Hypertension Dyslipidemias Risk Factors/prevalence Myocardial Infarction Heart Failure |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2019005001205&lng=en&tlng=en |
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