Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand
Background and Aim: A canine multi-drug resistance 1 (MDR1) nt230(del4) is a well-known inherited disorder that primarily affects collies and various herding breeds. The most recognized clinical implication for affected dogs is associated with an increased risk of multiple drug toxicity. To date, MD...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-11-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.14/November-2021/23.pdf |
| _version_ | 1818345159726727168 |
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| author | Chommanad Lerdkrai Nuch Phungphosop |
| author_facet | Chommanad Lerdkrai Nuch Phungphosop |
| author_sort | Chommanad Lerdkrai |
| collection | DOAJ |
| description | Background and Aim: A canine multi-drug resistance 1 (MDR1) nt230(del4) is a well-known inherited disorder that primarily affects collies and various herding breeds. The most recognized clinical implication for affected dogs is associated with an increased risk of multiple drug toxicity. To date, MDR1 gene mutations have been identified globally, especially in dogs from the USA and European countries. This study aimed to investigate the prevalence of MDR1 nt230(del4) in herding dog breeds and Thai Ridgebacks in Thailand.
Materials and Methods: We clarified the prevalence of MDR1 nt230(del4) in 263 dogs of eight purebred dog breeds in Thailand using an allele-specific multiplex polymerase chain reaction method and direct DNA sequencing.
Results: Rough Collies, Australian Shepherds, Shetland Sheepdogs, and Old English Sheepdogs were affected by the mutation with mutant allelic frequencies of 57.14%, 12.82%, 11.28%, and 8.33%, respectively. Among these populations, the prevalence of the MDR1 (+/–) genotype was 57.14% (12/21) for Rough Collies, 25.64% (10/39) for Australian Shepherds, 16.13% (15/93) for Shetland Sheepdogs, and 16.67% (2/12) for Old English Sheepdogs, whereas the MDR1 (–/–) mutation was only identified in Rough Collies and Shetland Sheepdogs, with prevalences of 28.57% (6/21) and 3.22% (3/93), respectively. However, the MDR1 nt230(del4) was not identified in Border Collies, German Shepherds, White Swiss Shepherds, or Thai Ridgebacks.
Conclusion: This study provides the current situation regarding MDR1 nt230(del4) in herding dog breeds in Thailand. In this survey, we investigated for the first time the status of MDR1 genotype in Thai Ridgebacks. These results are helpful for veterinarians managing effective therapeutic plans for commonly affected dog breeds, and these results will encourage all breeders to improve their selective breeding programs based on the MDR1 nt230(del4) status. |
| first_indexed | 2024-12-13T16:57:57Z |
| format | Article |
| id | doaj.art-98c98faf98f94212b6e46233aa4f1ae4 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-13T16:57:57Z |
| publishDate | 2021-11-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-98c98faf98f94212b6e46233aa4f1ae42022-12-21T23:37:53ZengVeterinary WorldVeterinary World0972-89882231-09162021-11-0114113015302010.14202/vetworld.2021.3015-3020Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in ThailandChommanad Lerdkrai0https://orcid.org/0000-0002-0527-9405Nuch Phungphosop1https://orcid.org/0000-0003-1237-0984Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.Background and Aim: A canine multi-drug resistance 1 (MDR1) nt230(del4) is a well-known inherited disorder that primarily affects collies and various herding breeds. The most recognized clinical implication for affected dogs is associated with an increased risk of multiple drug toxicity. To date, MDR1 gene mutations have been identified globally, especially in dogs from the USA and European countries. This study aimed to investigate the prevalence of MDR1 nt230(del4) in herding dog breeds and Thai Ridgebacks in Thailand. Materials and Methods: We clarified the prevalence of MDR1 nt230(del4) in 263 dogs of eight purebred dog breeds in Thailand using an allele-specific multiplex polymerase chain reaction method and direct DNA sequencing. Results: Rough Collies, Australian Shepherds, Shetland Sheepdogs, and Old English Sheepdogs were affected by the mutation with mutant allelic frequencies of 57.14%, 12.82%, 11.28%, and 8.33%, respectively. Among these populations, the prevalence of the MDR1 (+/–) genotype was 57.14% (12/21) for Rough Collies, 25.64% (10/39) for Australian Shepherds, 16.13% (15/93) for Shetland Sheepdogs, and 16.67% (2/12) for Old English Sheepdogs, whereas the MDR1 (–/–) mutation was only identified in Rough Collies and Shetland Sheepdogs, with prevalences of 28.57% (6/21) and 3.22% (3/93), respectively. However, the MDR1 nt230(del4) was not identified in Border Collies, German Shepherds, White Swiss Shepherds, or Thai Ridgebacks. Conclusion: This study provides the current situation regarding MDR1 nt230(del4) in herding dog breeds in Thailand. In this survey, we investigated for the first time the status of MDR1 genotype in Thai Ridgebacks. These results are helpful for veterinarians managing effective therapeutic plans for commonly affected dog breeds, and these results will encourage all breeders to improve their selective breeding programs based on the MDR1 nt230(del4) status.http://www.veterinaryworld.org/Vol.14/November-2021/23.pdfherding dog breedsmulti-drug resistance 1 genemutationp-glycoproteinthai ridgeback dogs |
| spellingShingle | Chommanad Lerdkrai Nuch Phungphosop Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand Veterinary World herding dog breeds multi-drug resistance 1 gene mutation p-glycoprotein thai ridgeback dogs |
| title | Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand |
| title_full | Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand |
| title_fullStr | Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand |
| title_full_unstemmed | Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand |
| title_short | Prevalence of the MDR1 gene mutation in herding dog breeds and Thai Ridgebacks in Thailand |
| title_sort | prevalence of the mdr1 gene mutation in herding dog breeds and thai ridgebacks in thailand |
| topic | herding dog breeds multi-drug resistance 1 gene mutation p-glycoprotein thai ridgeback dogs |
| url | http://www.veterinaryworld.org/Vol.14/November-2021/23.pdf |
| work_keys_str_mv | AT chommanadlerdkrai prevalenceofthemdr1genemutationinherdingdogbreedsandthairidgebacksinthailand AT nuchphungphosop prevalenceofthemdr1genemutationinherdingdogbreedsandthairidgebacksinthailand |