Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics
Microorganisms are confirmed to be closely related to the occurrence and development of cancers in human beings. However, there has been no published report detailing relationships between the oral microbiota and salivary adenoid cystic carcinoma (SACC). In this study, unstimulated saliva was collec...
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| Format: | Article |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.774453/full |
| _version_ | 1819179128815878144 |
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| author | Qian Jiang Qian Jiang Qian Jiang Xing Liu Xing Liu Xing Liu Qifen Yang Qifen Yang Qifen Yang Liang Chen Liang Chen Liang Chen Deqin Yang Deqin Yang Deqin Yang |
| author_facet | Qian Jiang Qian Jiang Qian Jiang Xing Liu Xing Liu Xing Liu Qifen Yang Qifen Yang Qifen Yang Liang Chen Liang Chen Liang Chen Deqin Yang Deqin Yang Deqin Yang |
| author_sort | Qian Jiang |
| collection | DOAJ |
| description | Microorganisms are confirmed to be closely related to the occurrence and development of cancers in human beings. However, there has been no published report detailing relationships between the oral microbiota and salivary adenoid cystic carcinoma (SACC). In this study, unstimulated saliva was collected from 13 SACC patients and 10 healthy controls. The microbial diversities, compositions and functions were comprehensively analyzed after 16S rRNA sequencing and whole-genome shotgun metagenomic sequencing. The alpha diversity showed no significant difference between SACC patients and healthy controls, while beta diversity showed a separation trend. The SACC patients showed higher abundances of Streptococcus and Rothia, while Prevotella and Alloprevotella were more abundant in healthy controls. The prevalent KEGG pathways, carbohydrate-active enzymes, antibiotic resistances and virulence factors as well as the biomarkers in SACC were determined by functional gene analysis. Our study preliminarily investigated the salivary microbiome of SACC patients compared with healthy controls and might be the basis for further studies on novel diagnostic and treatment strategies. |
| first_indexed | 2024-12-22T21:53:32Z |
| format | Article |
| id | doaj.art-98cc6c9b7c7a4432bfd6e3aa3c361a04 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-22T21:53:32Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-98cc6c9b7c7a4432bfd6e3aa3c361a042022-12-21T18:11:18ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-12-011110.3389/fcimb.2021.774453774453Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun MetagenomicsQian Jiang0Qian Jiang1Qian Jiang2Xing Liu3Xing Liu4Xing Liu5Qifen Yang6Qifen Yang7Qifen Yang8Liang Chen9Liang Chen10Liang Chen11Deqin Yang12Deqin Yang13Deqin Yang14Stomatological Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, ChinaChongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, ChinaStomatological Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, ChinaChongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, ChinaStomatological Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, ChinaChongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, ChinaStomatological Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, ChinaChongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, ChinaStomatological Hospital of Chongqing Medical University, Chongqing, China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, ChinaChongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, ChinaMicroorganisms are confirmed to be closely related to the occurrence and development of cancers in human beings. However, there has been no published report detailing relationships between the oral microbiota and salivary adenoid cystic carcinoma (SACC). In this study, unstimulated saliva was collected from 13 SACC patients and 10 healthy controls. The microbial diversities, compositions and functions were comprehensively analyzed after 16S rRNA sequencing and whole-genome shotgun metagenomic sequencing. The alpha diversity showed no significant difference between SACC patients and healthy controls, while beta diversity showed a separation trend. The SACC patients showed higher abundances of Streptococcus and Rothia, while Prevotella and Alloprevotella were more abundant in healthy controls. The prevalent KEGG pathways, carbohydrate-active enzymes, antibiotic resistances and virulence factors as well as the biomarkers in SACC were determined by functional gene analysis. Our study preliminarily investigated the salivary microbiome of SACC patients compared with healthy controls and might be the basis for further studies on novel diagnostic and treatment strategies.https://www.frontiersin.org/articles/10.3389/fcimb.2021.774453/fullmicrobiotasalivary adenoid cystic carcinomaoral cancer16S rRNA sequencingmetagenomicsbioinformatics |
| spellingShingle | Qian Jiang Qian Jiang Qian Jiang Xing Liu Xing Liu Xing Liu Qifen Yang Qifen Yang Qifen Yang Liang Chen Liang Chen Liang Chen Deqin Yang Deqin Yang Deqin Yang Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics Frontiers in Cellular and Infection Microbiology microbiota salivary adenoid cystic carcinoma oral cancer 16S rRNA sequencing metagenomics bioinformatics |
| title | Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics |
| title_full | Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics |
| title_fullStr | Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics |
| title_full_unstemmed | Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics |
| title_short | Salivary Microbiome in Adenoid Cystic Carcinoma Detected by 16S rRNA Sequencing and Shotgun Metagenomics |
| title_sort | salivary microbiome in adenoid cystic carcinoma detected by 16s rrna sequencing and shotgun metagenomics |
| topic | microbiota salivary adenoid cystic carcinoma oral cancer 16S rRNA sequencing metagenomics bioinformatics |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.774453/full |
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