Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS Biotyper
The honey bee, Apis mellifera, is critically important for the pollination of many economically important crops. Continued colony losses have called for a deeper understanding of both symbiotic and pathogenic microbial interactions, particularly as they relate to food storage and the pollination env...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/1082 |
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| author | Jaroslav Gasper Margarita Terentjeva Attila Kántor Eva Ivanišová Maciej Kluz Miroslava Kačániová |
| author_facet | Jaroslav Gasper Margarita Terentjeva Attila Kántor Eva Ivanišová Maciej Kluz Miroslava Kačániová |
| author_sort | Jaroslav Gasper |
| collection | DOAJ |
| description | The honey bee, Apis mellifera, is critically important for the pollination of many economically important crops. Continued colony losses have called for a deeper understanding of both symbiotic and pathogenic microbial interactions, particularly as they relate to food storage and the pollination environment. Therefore, the aim of this study was to explore and characterize the bacteria colonizing the alimentary tract of the native honey bees using MALDI TOF MS Biotyper. Content of the intestinal tract was cultured for isolation of Gram-negative, Gram-positive microorganisms and yeasts. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. Results showed that the most abundant genera in bees’ samples were Lactobacillus, Pseudomonas and Serratia. Altogether, 12 genera with 21 bacterial species and one yeast genus with two species were isolated. Bacteria were represented with Acidovorax facilis, Lactobacillus gasseri, L. amylovorus, L. kunkeei, L. fructivorans, Pseudomonas oryzihabitans, Ps. brenneri, Ps. indica, Micrococcus luteus, Serratia fonticola, Ser. marcescens, Ser. ureilytica, Hafnia alvei, Candida magnolia, Bacillus oleronius, B. horneckiae, Issatchenkia orientalis, Pantoea agglomerans, Enterobacter cloacae, Staphylococcus epidermidis, Staph. pasteuri, Shewanella profunda. The results of the study shows that the microflora of the bees gut is heterogenic and depend of locality and resources of environment for bees. |
| first_indexed | 2024-04-24T11:30:04Z |
| format | Article |
| id | doaj.art-98cde6e49b35433ca86a059f341517ff |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:30:04Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-98cde6e49b35433ca86a059f341517ff2024-04-10T10:48:43ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015011921921082Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS BiotyperJaroslav Gasper0Margarita Terentjeva1Attila Kántor2Eva Ivanišová3Maciej Kluz4Miroslava Kačániová5Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia Latvia University of Agriculture, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, Jelgava, LV-3004, K. Helmaņa iela 8, LatviaSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia University of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, PolandSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia The honey bee, Apis mellifera, is critically important for the pollination of many economically important crops. Continued colony losses have called for a deeper understanding of both symbiotic and pathogenic microbial interactions, particularly as they relate to food storage and the pollination environment. Therefore, the aim of this study was to explore and characterize the bacteria colonizing the alimentary tract of the native honey bees using MALDI TOF MS Biotyper. Content of the intestinal tract was cultured for isolation of Gram-negative, Gram-positive microorganisms and yeasts. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. Results showed that the most abundant genera in bees’ samples were Lactobacillus, Pseudomonas and Serratia. Altogether, 12 genera with 21 bacterial species and one yeast genus with two species were isolated. Bacteria were represented with Acidovorax facilis, Lactobacillus gasseri, L. amylovorus, L. kunkeei, L. fructivorans, Pseudomonas oryzihabitans, Ps. brenneri, Ps. indica, Micrococcus luteus, Serratia fonticola, Ser. marcescens, Ser. ureilytica, Hafnia alvei, Candida magnolia, Bacillus oleronius, B. horneckiae, Issatchenkia orientalis, Pantoea agglomerans, Enterobacter cloacae, Staphylococcus epidermidis, Staph. pasteuri, Shewanella profunda. The results of the study shows that the microflora of the bees gut is heterogenic and depend of locality and resources of environment for bees.https://spasb.ro/index.php/public_html/article/view/1082apis meliferagut microbiotaidentificationmaldi tof ms biotyper |
| spellingShingle | Jaroslav Gasper Margarita Terentjeva Attila Kántor Eva Ivanišová Maciej Kluz Miroslava Kačániová Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS Biotyper Scientific Papers Animal Science and Biotechnologies apis melifera gut microbiota identification maldi tof ms biotyper |
| title | Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS Biotyper |
| title_full | Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS Biotyper |
| title_fullStr | Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS Biotyper |
| title_full_unstemmed | Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS Biotyper |
| title_short | Identification of Apis mellifera Gut Microbiota with MALDI-TOF MS Biotyper |
| title_sort | identification of apis mellifera gut microbiota with maldi tof ms biotyper |
| topic | apis melifera gut microbiota identification maldi tof ms biotyper |
| url | https://spasb.ro/index.php/public_html/article/view/1082 |
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