Detection of Hepatits C Virus (HCV) by ELISA, RIBA and Reverse Transcriptase- Polymerase Chain Reaction (RT-PCR) Technique among Kidney dialysis patients in Nineveh Governorate/Iraq.
Introduction: the silent disease (Hepatitis C virus) is considered as one of the major causes of chronic hepatitis and cirrhosis, hence the aims of study to detect Hepatitis C virus among hemodialysis and peritoneal dialysis in Nineveh Governorate/Iraq. Patients and Methods: (80) blood samples were...
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| Format: | Article |
| Language: | English |
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University of Thi-Qar
2019-06-01
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| Series: | مجلة علوم ذي قار |
| Online Access: | https://jsci.utq.edu.iq/index.php/main/article/view/439 |
| Summary: | Introduction: the silent disease (Hepatitis C virus) is considered as one of the major causes of chronic
hepatitis and cirrhosis, hence the aims of study to detect Hepatitis C virus among hemodialysis and peritoneal
dialysis in Nineveh Governorate/Iraq.
Patients and Methods: (80) blood samples were collected from dialysis patients 46 (57.5%) males, (42.5%)
females at age (15-60) years, the average age (37.5) years, include (50) heamodialysis patients, (30)
peritoneal patients and (100) person from health blood donors as a control group.
We used ELISA version 3.0 to detect anti-HCV and confirm the positive and equivocal results by
Recombinant Immunoblot Assay version 3.0 (RIBA HCV 3.0), and used Reverse Transcribtase Polymerase
Chain reaction (RT-PCR) to confirm positive and equivocal results by RIBA technique.
Results: the current study showed significant differences (P?0.05) between percentage of ant i-HCV and
HCV RNA between heamodialysis, peritoneal dialysis and control group. The percentage of anti-HCV when
tested by ELISA in serum of heamodialysis patients was (48%) positive, (48%) negative, (4%) equivocal,
while (2%) positive (98%) in serum of peritoneal dialysis, (1%) positive, (99%) negative in control group.
We used RIBA technique to confirm positive and equivocal results in ELISA the results showed (44%)
positive, (52%) negative and (4%) equivocal between heamodialysis patients, (2%) positive, (98%) negative
between peritoneal dialysis, while showed (1%) positive, (99%) between control group.
We used (RT-PCR) to confirm positive and equivocal results in RIBA technique; it showed (50%) positive
between heamodialysis patients, (2%) positive in peritoneal dialysis, (1%) positive in control group.
Conclusion: the prevalence of Hepatitis C virus was higher in Heamodialysis than peritoneal dialysis in
Nineveh Governorate/Iraq.
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| ISSN: | 1991-8690 2709-0256 |