Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

Abstract Background RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every molecule amplifies equally, causing some to be overre...

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Bibliographic Details
Main Authors: Yu Fu, Pei-Hsuan Wu, Timothy Beane, Phillip D. Zamore, Zhiping Weng
Format: Article
Language:English
Published: BMC 2018-07-01
Series:BMC Genomics
Subjects:
RNA-seq
Small RNA-seq
Unique molecular identifier
UMI
PCR duplicates
PCR cycle
Online Access:http://link.springer.com/article/10.1186/s12864-018-4933-1

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