In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes
Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, u...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/7/868 |
| _version_ | 1797562942915346432 |
|---|---|
| author | Ivan Kashkan Ksenia Timofeyenko Eva Kollárová Kamil Růžička |
| author_facet | Ivan Kashkan Ksenia Timofeyenko Eva Kollárová Kamil Růžička |
| author_sort | Ivan Kashkan |
| collection | DOAJ |
| description | Rapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS. |
| first_indexed | 2024-03-10T18:35:45Z |
| format | Article |
| id | doaj.art-98d29b92642743bf94bf11eaa7ea3f00 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:35:45Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-98d29b92642743bf94bf11eaa7ea3f002023-11-20T06:14:36ZengMDPI AGPlants2223-77472020-07-019786810.3390/plants9070868In Vivo Reporters for Visualizing Alternative Splicing of Hormonal GenesIvan Kashkan0Ksenia Timofeyenko1Eva Kollárová2Kamil Růžička3Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech RepublicLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech RepublicFunctional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 625 00 Brno, Czech RepublicLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Czech Academy of Sciences, 165 02 Prague, Czech RepublicRapid progress in plant molecular biology in recent years has uncovered the main players in hormonal pathways and characterized transcriptomic networks associated with hormonal response. However, the role of RNA processing, in particular alternative splicing (AS), remains largely unexplored. Here, using example genes involved in cytokinin signaling, brassinosteroid synthesis and auxin transport, we present a set of reporters devised to visualize their AS events in vivo. These reporters show a differential tissue-specific expression of certain transcripts and reveal that expression of some of the them can be changed by the application of the exogenous hormone. Finally, based on the characterized AS event of the PIN7 auxin efflux carrier, we designed a system that allows a rapid genetic screening for the factors upstream of this AS event. Our innovative toolset can be therefore highly useful for exploring novel regulatory nodes of hormonal pathways and potentially helpful for plant researchers focusing on developmental aspects of AS.https://www.mdpi.com/2223-7747/9/7/868alternative splicingauxincytokininsbrassinosteroids<i>AHP6</i><i>DWF4</i> |
| spellingShingle | Ivan Kashkan Ksenia Timofeyenko Eva Kollárová Kamil Růžička In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes Plants alternative splicing auxin cytokinins brassinosteroids <i>AHP6</i> <i>DWF4</i> |
| title | In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes |
| title_full | In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes |
| title_fullStr | In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes |
| title_full_unstemmed | In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes |
| title_short | In Vivo Reporters for Visualizing Alternative Splicing of Hormonal Genes |
| title_sort | in vivo reporters for visualizing alternative splicing of hormonal genes |
| topic | alternative splicing auxin cytokinins brassinosteroids <i>AHP6</i> <i>DWF4</i> |
| url | https://www.mdpi.com/2223-7747/9/7/868 |
| work_keys_str_mv | AT ivankashkan invivoreportersforvisualizingalternativesplicingofhormonalgenes AT kseniatimofeyenko invivoreportersforvisualizingalternativesplicingofhormonalgenes AT evakollarova invivoreportersforvisualizingalternativesplicingofhormonalgenes AT kamilruzicka invivoreportersforvisualizingalternativesplicingofhormonalgenes |