Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological Measurements

In this study, the enzyme-induced and acid coagulation of raw milk samples were investigated, and these two processes were monitored by determining the changes in dielectric and rheological behavior to see if any correlations could be found between these two properties. For the enzyme-induced experi...

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Main Authors: Réka Dobozi, Zoltán Péter Jákói, József Csanádi, Sándor Beszédes
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/13/10/6185
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author Réka Dobozi
Zoltán Péter Jákói
József Csanádi
Sándor Beszédes
author_facet Réka Dobozi
Zoltán Péter Jákói
József Csanádi
Sándor Beszédes
author_sort Réka Dobozi
collection DOAJ
description In this study, the enzyme-induced and acid coagulation of raw milk samples were investigated, and these two processes were monitored by determining the changes in dielectric and rheological behavior to see if any correlations could be found between these two properties. For the enzyme-induced experiments, 250 cm<sup>3</sup> of raw milk sample and industrial chymosin were used, and during the acid coagulation, industrial thermophile yogurt culture was added to the same amount of milk. The apparent viscosity was monitored with a vibro viscometer, and the dielectric constant was measured in a frequency range of 200–2400 MHz with an open-ended dielectric assessment kit (DAK) connected to a vector network analyzer (VNA). Our experimental findings revealed that during both enzyme-induced and acid coagulation, two distinct phases could be found in terms of dielectric and rheological properties, and regardless of the molecular mechanisms behind the aggregation of casein micelles, a strong correlation (with R<sup>2</sup>-values ranging between 0.967 and 0.992) can be found between the extent of the apparent viscosity and dielectric constant, suggesting that dielectric measurements might be adequately used in these dairy processing technologies. These results verified the applicability of the non-destructive dielectric measurements in dairy technologies.
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spelling doaj.art-98d5bf3662624561ba383bb2bc5ce5e42023-11-18T00:21:50ZengMDPI AGApplied Sciences2076-34172023-05-011310618510.3390/app13106185Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological MeasurementsRéka Dobozi0Zoltán Péter Jákói1József Csanádi2Sándor Beszédes3Department of Food Engineering, Faculty of Engineering, University of Szeged, H-6725 Szeged, HungaryDepartment of Biosystems Engineering, Faculty of Engineering, University of Szeged, H-6725 Szeged, HungaryDepartment of Food Engineering, Faculty of Engineering, University of Szeged, H-6725 Szeged, HungaryDepartment of Biosystems Engineering, Faculty of Engineering, University of Szeged, H-6725 Szeged, HungaryIn this study, the enzyme-induced and acid coagulation of raw milk samples were investigated, and these two processes were monitored by determining the changes in dielectric and rheological behavior to see if any correlations could be found between these two properties. For the enzyme-induced experiments, 250 cm<sup>3</sup> of raw milk sample and industrial chymosin were used, and during the acid coagulation, industrial thermophile yogurt culture was added to the same amount of milk. The apparent viscosity was monitored with a vibro viscometer, and the dielectric constant was measured in a frequency range of 200–2400 MHz with an open-ended dielectric assessment kit (DAK) connected to a vector network analyzer (VNA). Our experimental findings revealed that during both enzyme-induced and acid coagulation, two distinct phases could be found in terms of dielectric and rheological properties, and regardless of the molecular mechanisms behind the aggregation of casein micelles, a strong correlation (with R<sup>2</sup>-values ranging between 0.967 and 0.992) can be found between the extent of the apparent viscosity and dielectric constant, suggesting that dielectric measurements might be adequately used in these dairy processing technologies. These results verified the applicability of the non-destructive dielectric measurements in dairy technologies.https://www.mdpi.com/2076-3417/13/10/6185acid coagulationenzyme-induced coagulationdielectric propertiesclot formationrheological properties
spellingShingle Réka Dobozi
Zoltán Péter Jákói
József Csanádi
Sándor Beszédes
Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological Measurements
Applied Sciences
acid coagulation
enzyme-induced coagulation
dielectric properties
clot formation
rheological properties
title Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological Measurements
title_full Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological Measurements
title_fullStr Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological Measurements
title_full_unstemmed Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological Measurements
title_short Investigating the Acid- and Enzyme-Induced Coagulation of Raw Milk Using Dielectric and Rheological Measurements
title_sort investigating the acid and enzyme induced coagulation of raw milk using dielectric and rheological measurements
topic acid coagulation
enzyme-induced coagulation
dielectric properties
clot formation
rheological properties
url https://www.mdpi.com/2076-3417/13/10/6185
work_keys_str_mv AT rekadobozi investigatingtheacidandenzymeinducedcoagulationofrawmilkusingdielectricandrheologicalmeasurements
AT zoltanpeterjakoi investigatingtheacidandenzymeinducedcoagulationofrawmilkusingdielectricandrheologicalmeasurements
AT jozsefcsanadi investigatingtheacidandenzymeinducedcoagulationofrawmilkusingdielectricandrheologicalmeasurements
AT sandorbeszedes investigatingtheacidandenzymeinducedcoagulationofrawmilkusingdielectricandrheologicalmeasurements