The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat Model
Prior animal models have shown that rats sustaining 3-second immediate spinal cord compression had significantly better functional recovery and smaller lesion volumes than rats subjected to compression times of 1 hour, 6 hours, 3 weeks, and 10 weeks after spinal cord injury. We compare locomotor rat...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-07-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/4416 |
| _version_ | 1811280926996430848 |
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| author | Seyed Behzad Jazayeri Masoumeh Firouzi Shayan Abdollah Zadegan Niloufar Saeedi Elham Pirouz Mohsen Nategh Issa Jahanzad Ahmad Mohebbi Ashtiani Vafa Rahimi-Movaghar |
| author_facet | Seyed Behzad Jazayeri Masoumeh Firouzi Shayan Abdollah Zadegan Niloufar Saeedi Elham Pirouz Mohsen Nategh Issa Jahanzad Ahmad Mohebbi Ashtiani Vafa Rahimi-Movaghar |
| author_sort | Seyed Behzad Jazayeri |
| collection | DOAJ |
| description | Prior animal models have shown that rats sustaining 3-second immediate spinal cord compression had significantly better functional recovery and smaller lesion volumes than rats subjected to compression times of 1 hour, 6 hours, 3 weeks, and 10 weeks after spinal cord injury. We compare locomotor rating scales and spinal cord histopathology after 3 seconds and 10 minute compression times. . Ten rats were assigned into two early (3-second) and late (10-minute) compressive surgery groups. Compressive injury was produced using an aneurysmal clip method. Rats were followed-up for 11 weeks, and behavioral assessment was done by inclined plane test and tail-flick reflex. At the end of the study, the rats were sacrificed, and spinal cord specimens were studied in light and EM. Basso, Beattie and Bresnahan (BBB) locomotor rating scales were significantly better in the early compression group after the 4th week of evaluation (P<0.05) and persisted throughout the remainder of the study. Histopathology demonstrated decreased normal tissue, more severe gliosis and cystic formation in the late group compared to the early group (P<0.05). In EM study, injuries in the late group including injury to the myelin and axon were more severe than the early compression group, and there was more cytoplasmic edema in the late compression group. Spinal cord injury secondary to 3-second compression improves functional motor recovery, spares more functional tissue, and is associated with less intracellular edema, less myelin and axon damage and more myelin regeneration in rats compared to those with 10 minutes of compression. Inclined plane test and tail-flick reflex had no significant difference. |
| first_indexed | 2024-04-13T01:23:18Z |
| format | Article |
| id | doaj.art-98d8eca1460c4d089014fc608e49ed3e |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-04-13T01:23:18Z |
| publishDate | 2013-07-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-98d8eca1460c4d089014fc608e49ed3e2022-12-22T03:08:41ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942013-07-015174369The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat ModelSeyed Behzad Jazayeri0Masoumeh Firouzi1Shayan Abdollah Zadegan2Niloufar Saeedi3Elham Pirouz4Mohsen Nategh5Issa Jahanzad6Ahmad Mohebbi Ashtiani7Vafa Rahimi-Movaghar8Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Student’s Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.Tissue Repair Laboratory, Institute of Biochemistry and Biophysics (IBB), Tehran University of Medical Sciences, Tehran, Iran.Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Research Centre for Neural Repair, Tehran University of Medical Sciences, Tehran, Iran.Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran.Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran.Research Centre for Neural Repair, Tehran University of Medical Sciences, Tehran, Iran.Department of Pathology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Research Centre for Neural Repair, Tehran University of Medical Sciences, Tehran, Iran.Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Research Centre for Neural Repair, Tehran University of Medical Sciences, Tehran, Iran.Prior animal models have shown that rats sustaining 3-second immediate spinal cord compression had significantly better functional recovery and smaller lesion volumes than rats subjected to compression times of 1 hour, 6 hours, 3 weeks, and 10 weeks after spinal cord injury. We compare locomotor rating scales and spinal cord histopathology after 3 seconds and 10 minute compression times. . Ten rats were assigned into two early (3-second) and late (10-minute) compressive surgery groups. Compressive injury was produced using an aneurysmal clip method. Rats were followed-up for 11 weeks, and behavioral assessment was done by inclined plane test and tail-flick reflex. At the end of the study, the rats were sacrificed, and spinal cord specimens were studied in light and EM. Basso, Beattie and Bresnahan (BBB) locomotor rating scales were significantly better in the early compression group after the 4th week of evaluation (P<0.05) and persisted throughout the remainder of the study. Histopathology demonstrated decreased normal tissue, more severe gliosis and cystic formation in the late group compared to the early group (P<0.05). In EM study, injuries in the late group including injury to the myelin and axon were more severe than the early compression group, and there was more cytoplasmic edema in the late compression group. Spinal cord injury secondary to 3-second compression improves functional motor recovery, spares more functional tissue, and is associated with less intracellular edema, less myelin and axon damage and more myelin regeneration in rats compared to those with 10 minutes of compression. Inclined plane test and tail-flick reflex had no significant difference.https://acta.tums.ac.ir/index.php/acta/article/view/4416BBBDecompressionElectron microscopyHistopathologySpinal cord injury |
| spellingShingle | Seyed Behzad Jazayeri Masoumeh Firouzi Shayan Abdollah Zadegan Niloufar Saeedi Elham Pirouz Mohsen Nategh Issa Jahanzad Ahmad Mohebbi Ashtiani Vafa Rahimi-Movaghar The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat Model Acta Medica Iranica BBB Decompression Electron microscopy Histopathology Spinal cord injury |
| title | The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat Model |
| title_full | The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat Model |
| title_fullStr | The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat Model |
| title_full_unstemmed | The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat Model |
| title_short | The Effect of Timing of Decompression on Neurologic Recovery and Histopathologic Findings After Spinal Cord Compression in a Rat Model |
| title_sort | effect of timing of decompression on neurologic recovery and histopathologic findings after spinal cord compression in a rat model |
| topic | BBB Decompression Electron microscopy Histopathology Spinal cord injury |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/4416 |
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