Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1)
AbstractWe used cell-free culture filtrate of Pseudomonas aeruginosa as a reducing mediator of AgNO3 to silvernanoparticles (AgNPs) and possibly used as a potential anticancer agent against thyroid cancer cells (TPC1). The bio-generation of AgNPs was firmly established by taking a UV spectrum at 380...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1687495 |
| _version_ | 1797788822107324416 |
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| author | Jinmei Yang Qiang Wang Chunxiao Wang Ruiyi Yang Mukhtar Ahmed Subramanian Kumaran Periyannan Velu Bo Li |
| author_facet | Jinmei Yang Qiang Wang Chunxiao Wang Ruiyi Yang Mukhtar Ahmed Subramanian Kumaran Periyannan Velu Bo Li |
| author_sort | Jinmei Yang |
| collection | DOAJ |
| description | AbstractWe used cell-free culture filtrate of Pseudomonas aeruginosa as a reducing mediator of AgNO3 to silvernanoparticles (AgNPs) and possibly used as a potential anticancer agent against thyroid cancer cells (TPC1). The bio-generation of AgNPs was firmly established by taking a UV spectrum at 380–500 nm wavelength. The Fourier transform spectrum analysis reveals the association of alcohol, phenol and aromatic functional groups with P. aeruginosa synthesized AgNPs (Ps-AgNPs). By observing under transmission electron microscopy (TEM), the size and structure of the Ps-AgNPs were characterized as the size was 30–70 nm and spherical in shape. The concentration-dependent cytotoxicity of Ps-AgNPs on TPC1 cells was observed and IC50 value was calculated. The alteration of oxidative and antioxidant biomarkers in Ps-AgNPs treated cells were observed. The induced apoptosis was determined by staining the Ps-AgNPs treated cells with DCFH-DA, Rh-123 dye, Acridine Orange (AO) and ethidium bromide (EtBr). Increased level of intracellular reactive oxygen species (ROS) generation and decreased level of mitochondrial membrane potential was observed in Ps-AgNPs treated TPC1 cells. Moreover, the apoptotic morphological changes were explored, which indicates increased apoptosis by inducing cell membrane damage in Ps-AgNPs treated cells. This biogenic approach will enable an effective and significant improvement in nano-oncotherapy. |
| first_indexed | 2024-03-13T01:40:56Z |
| format | Article |
| id | doaj.art-98d90ce93b0c4878b25142bca1637929 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-13T01:40:56Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-98d90ce93b0c4878b25142bca16379292023-07-03T14:04:56ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148180080910.1080/21691401.2019.1687495Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1)Jinmei Yang0Qiang Wang1Chunxiao Wang2Ruiyi Yang3Mukhtar Ahmed4Subramanian Kumaran5Periyannan Velu6Bo Li7Physical Examination Center, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaPhysical Examination Center, The Fourth Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of General Surgery, The Fourth Affiliated Hospital of Kunming Medical University, Kunming, ChinaPhysical Examination Center, The First Affiliated Hospital of Kunming Medical University, Kunming, ChinaDepartment of Zoology, College of Science, King Saud University, Riyadh, Kingdom of Saudi ArabiaDepartment of Biotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology (Deemed to Be University), Chennai, IndiaScigen Research and Innovation Pvt. Ltd, Periyar Technology Business Incubator, Thanjavur, IndiaDepartment of General Surgery, The Fourth Affiliated Hospital of Kunming Medical University, Kunming, ChinaAbstractWe used cell-free culture filtrate of Pseudomonas aeruginosa as a reducing mediator of AgNO3 to silvernanoparticles (AgNPs) and possibly used as a potential anticancer agent against thyroid cancer cells (TPC1). The bio-generation of AgNPs was firmly established by taking a UV spectrum at 380–500 nm wavelength. The Fourier transform spectrum analysis reveals the association of alcohol, phenol and aromatic functional groups with P. aeruginosa synthesized AgNPs (Ps-AgNPs). By observing under transmission electron microscopy (TEM), the size and structure of the Ps-AgNPs were characterized as the size was 30–70 nm and spherical in shape. The concentration-dependent cytotoxicity of Ps-AgNPs on TPC1 cells was observed and IC50 value was calculated. The alteration of oxidative and antioxidant biomarkers in Ps-AgNPs treated cells were observed. The induced apoptosis was determined by staining the Ps-AgNPs treated cells with DCFH-DA, Rh-123 dye, Acridine Orange (AO) and ethidium bromide (EtBr). Increased level of intracellular reactive oxygen species (ROS) generation and decreased level of mitochondrial membrane potential was observed in Ps-AgNPs treated TPC1 cells. Moreover, the apoptotic morphological changes were explored, which indicates increased apoptosis by inducing cell membrane damage in Ps-AgNPs treated cells. This biogenic approach will enable an effective and significant improvement in nano-oncotherapy.https://www.tandfonline.com/doi/10.1080/21691401.2019.1687495Silver nanoparticlesthyroid cancerTPC1 cellslipid peroxidationnano-oncotherapy |
| spellingShingle | Jinmei Yang Qiang Wang Chunxiao Wang Ruiyi Yang Mukhtar Ahmed Subramanian Kumaran Periyannan Velu Bo Li Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1) Artificial Cells, Nanomedicine, and Biotechnology Silver nanoparticles thyroid cancer TPC1 cells lipid peroxidation nano-oncotherapy |
| title | Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1) |
| title_full | Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1) |
| title_fullStr | Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1) |
| title_full_unstemmed | Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1) |
| title_short | Pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ROS mediated apoptosis in thyroid cancer cell line (TPC1) |
| title_sort | pseudomonas aeruginosa synthesized silver nanoparticles inhibit cell proliferation and induce ros mediated apoptosis in thyroid cancer cell line tpc1 |
| topic | Silver nanoparticles thyroid cancer TPC1 cells lipid peroxidation nano-oncotherapy |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1687495 |
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