Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain
Mycosis is often misdiagnosed as Tuberculosis (TB) with negative sputum smear results or Tuberculosis recurrent. In the last two-decade, mycosis increases dramatically as HIV and immunocompromised incidence rates increased. Even though, the prevalence data of mycosis in TB patient is less researched...
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| Format: | Article |
| Language: | Indonesian |
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Universitas Muhammadiyah Surabaya
2023-07-01
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| Series: | Qanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya |
| Subjects: | |
| Online Access: | https://journal.um-surabaya.ac.id/index.php/qanunmedika/article/view/18606 |
| _version_ | 1797771645761355776 |
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| author | Ratna Kusumawati Eddy Bagus Wasito Arthur Pohan Kawilarang Eko Budi Koendhori |
| author_facet | Ratna Kusumawati Eddy Bagus Wasito Arthur Pohan Kawilarang Eko Budi Koendhori |
| author_sort | Ratna Kusumawati |
| collection | DOAJ |
| description | Mycosis is often misdiagnosed as Tuberculosis (TB) with negative sputum smear results or Tuberculosis recurrent. In the last two-decade, mycosis increases dramatically as HIV and immunocompromised incidence rates increased. Even though, the prevalence data of mycosis in TB patient is less researched, the identification using KOH often give a false negative result. To compare the result of fungal identification using KOH and PAS staining. This study is observational analytical using a cross-sectional design with 29 TB samples that have been diagnosed with Ziehl-Neelsen (ZN) stain positive in RSUD Dr. Soetomo. The sputum of every sample was colored with KOH and PAS and observed by the researcher and microbiology analyst. The results of this study were 22 sputa identified with Candida sp positive and 12 sputa with non-Candida positive using KOH staining. Meanwhile, using PAS staining, there were 25 sputa identified as Candida sp positive and 22 sputa as non-Candida positive. The congruency test between KOH and PAS has a fair result for both identifying Candida sp (κ = 0.298; significance = 0.069) and non-Candida (κ = 0.240; significance = 0.095). The identification of candida and non-candida in TB patients using KOH and PAS has a fair congruency result. The PAS staining has better identification results both in identifying candida and non-candida rather than KOH staining. |
| first_indexed | 2024-03-12T21:40:35Z |
| format | Article |
| id | doaj.art-98d9dc6be5d2411c9d89a9096cdb4a06 |
| institution | Directory Open Access Journal |
| issn | 2541-2272 2548-9526 |
| language | Indonesian |
| last_indexed | 2024-03-12T21:40:35Z |
| publishDate | 2023-07-01 |
| publisher | Universitas Muhammadiyah Surabaya |
| record_format | Article |
| series | Qanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya |
| spelling | doaj.art-98d9dc6be5d2411c9d89a9096cdb4a062023-07-27T01:42:12ZindUniversitas Muhammadiyah SurabayaQanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya2541-22722548-95262023-07-017210.30651/jqm.v7i2.186065794Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stainRatna Kusumawati0Eddy Bagus Wasito1Arthur Pohan Kawilarang2Eko Budi Koendhori3Dr Soetomo Academic Hospital - Airlangga UniversityDr Soetomo Academic Hospital - Airlangga UniversityDr Soetomo Academic Hospital - Airlangga UniversityDr Soetomo Academic Hospital - Airlangga UniversityMycosis is often misdiagnosed as Tuberculosis (TB) with negative sputum smear results or Tuberculosis recurrent. In the last two-decade, mycosis increases dramatically as HIV and immunocompromised incidence rates increased. Even though, the prevalence data of mycosis in TB patient is less researched, the identification using KOH often give a false negative result. To compare the result of fungal identification using KOH and PAS staining. This study is observational analytical using a cross-sectional design with 29 TB samples that have been diagnosed with Ziehl-Neelsen (ZN) stain positive in RSUD Dr. Soetomo. The sputum of every sample was colored with KOH and PAS and observed by the researcher and microbiology analyst. The results of this study were 22 sputa identified with Candida sp positive and 12 sputa with non-Candida positive using KOH staining. Meanwhile, using PAS staining, there were 25 sputa identified as Candida sp positive and 22 sputa as non-Candida positive. The congruency test between KOH and PAS has a fair result for both identifying Candida sp (κ = 0.298; significance = 0.069) and non-Candida (κ = 0.240; significance = 0.095). The identification of candida and non-candida in TB patients using KOH and PAS has a fair congruency result. The PAS staining has better identification results both in identifying candida and non-candida rather than KOH staining.https://journal.um-surabaya.ac.id/index.php/qanunmedika/article/view/18606koh, pas, candida, non-candida, tb |
| spellingShingle | Ratna Kusumawati Eddy Bagus Wasito Arthur Pohan Kawilarang Eko Budi Koendhori Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain Qanun Medika: Jurnal Kedokteran Fakultas Kedokteran Universitas Muhammadiyah Surabaya koh, pas, candida, non-candida, tb |
| title | Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain |
| title_full | Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain |
| title_fullStr | Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain |
| title_full_unstemmed | Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain |
| title_short | Comparative evaluation between KOH and PAS stain of fungal examination result on lung Tuberculosis patients’ sputum with positive Ziehl-Neelsen stain |
| title_sort | comparative evaluation between koh and pas stain of fungal examination result on lung tuberculosis patients sputum with positive ziehl neelsen stain |
| topic | koh, pas, candida, non-candida, tb |
| url | https://journal.um-surabaya.ac.id/index.php/qanunmedika/article/view/18606 |
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