Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats
Olive-derived products, such as virgin olive oil (EVOO) and/or olive leaf extracts (OLE), exert anti-inflammatory, insulin-sensitizing and antihypertensive properties and may be useful for stabilizing omega 3 fatty acids (n-3 PUFA) due to their high content in antioxidant compounds. In this study, t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/7/1066 |
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| author | Daniel González-Hedström María de la Fuente-Fernández Teresa Priego Ana Isabel Martín Sara Amor Asunción López-Calderón Antonio Manuel Inarejos-García Ángel Luís García-Villalón Miriam Granado |
| author_facet | Daniel González-Hedström María de la Fuente-Fernández Teresa Priego Ana Isabel Martín Sara Amor Asunción López-Calderón Antonio Manuel Inarejos-García Ángel Luís García-Villalón Miriam Granado |
| author_sort | Daniel González-Hedström |
| collection | DOAJ |
| description | Olive-derived products, such as virgin olive oil (EVOO) and/or olive leaf extracts (OLE), exert anti-inflammatory, insulin-sensitizing and antihypertensive properties and may be useful for stabilizing omega 3 fatty acids (n-3 PUFA) due to their high content in antioxidant compounds. In this study, the addition of OLE 4:0.15 (<i>w</i><i>/w</i>) to a mixture of algae oil (AO) rich in n-3 PUFA and EVOO (25:75, <i>w</i>/<i>w</i>) prevents peroxides formation after 12 months of storage at 30 °C. Furthermore, the treatment with the oil mixture (2.5 mL/Kg) and OLE (100 mg/Kg) to 24 month old Wistar rats for 21 days improved the lipid profile, increased the HOMA-IR and decreased the serum levels of miRNAs 21 and 146a. Treatment with this new nutraceutical also prevented age-induced insulin resistance in the liver, gastrocnemius and visceral adipose tissue by decreasing the mRNA levels of inflammatory and oxidative stress markers. Oil mixture + OLE also attenuated the age-induced alterations in vascular function and prevented muscle loss by decreasing the expression of sarcopenia-related markers. In conclusion, treatment with a new nutraceutical based on a mixture of EVOO, AO and OLE is a useful strategy for improving the stability of n-3 PUFA in the final product and to attenuate the cardiometabolic and muscular disorders associated with aging. |
| first_indexed | 2024-03-10T09:48:07Z |
| format | Article |
| id | doaj.art-98daa1f001a14c28b9849913483a2431 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T09:48:07Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-98daa1f001a14c28b9849913483a24312023-11-22T03:04:50ZengMDPI AGAntioxidants2076-39212021-07-01107106610.3390/antiox10071066Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in RatsDaniel González-Hedström0María de la Fuente-Fernández1Teresa Priego2Ana Isabel Martín3Sara Amor4Asunción López-Calderón5Antonio Manuel Inarejos-García6Ángel Luís García-Villalón7Miriam Granado8Departamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, 28029 Madrid, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, 28029 Madrid, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, 28029 Madrid, SpainPharmactive Biotech Products S.L. Parque Científico de Madrid, Avenida del Doctor Severo Ochoa, 37 Local 4J, 28049 Madrid, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, SpainDepartamento de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, 28029 Madrid, SpainOlive-derived products, such as virgin olive oil (EVOO) and/or olive leaf extracts (OLE), exert anti-inflammatory, insulin-sensitizing and antihypertensive properties and may be useful for stabilizing omega 3 fatty acids (n-3 PUFA) due to their high content in antioxidant compounds. In this study, the addition of OLE 4:0.15 (<i>w</i><i>/w</i>) to a mixture of algae oil (AO) rich in n-3 PUFA and EVOO (25:75, <i>w</i>/<i>w</i>) prevents peroxides formation after 12 months of storage at 30 °C. Furthermore, the treatment with the oil mixture (2.5 mL/Kg) and OLE (100 mg/Kg) to 24 month old Wistar rats for 21 days improved the lipid profile, increased the HOMA-IR and decreased the serum levels of miRNAs 21 and 146a. Treatment with this new nutraceutical also prevented age-induced insulin resistance in the liver, gastrocnemius and visceral adipose tissue by decreasing the mRNA levels of inflammatory and oxidative stress markers. Oil mixture + OLE also attenuated the age-induced alterations in vascular function and prevented muscle loss by decreasing the expression of sarcopenia-related markers. In conclusion, treatment with a new nutraceutical based on a mixture of EVOO, AO and OLE is a useful strategy for improving the stability of n-3 PUFA in the final product and to attenuate the cardiometabolic and muscular disorders associated with aging.https://www.mdpi.com/2076-3921/10/7/1066agingolive leaf extractomega 3 fatty acidsalgae oilextra virgin olive oilinsulin resistance |
| spellingShingle | Daniel González-Hedström María de la Fuente-Fernández Teresa Priego Ana Isabel Martín Sara Amor Asunción López-Calderón Antonio Manuel Inarejos-García Ángel Luís García-Villalón Miriam Granado Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats Antioxidants aging olive leaf extract omega 3 fatty acids algae oil extra virgin olive oil insulin resistance |
| title | Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats |
| title_full | Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats |
| title_fullStr | Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats |
| title_full_unstemmed | Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats |
| title_short | Addition of Olive Leaf Extract to a Mixture of Algae and Extra Virgin Olive Oils Decreases Fatty Acid Oxidation and Synergically Attenuates Age-Induced Hypertension, Sarcopenia and Insulin Resistance in Rats |
| title_sort | addition of olive leaf extract to a mixture of algae and extra virgin olive oils decreases fatty acid oxidation and synergically attenuates age induced hypertension sarcopenia and insulin resistance in rats |
| topic | aging olive leaf extract omega 3 fatty acids algae oil extra virgin olive oil insulin resistance |
| url | https://www.mdpi.com/2076-3921/10/7/1066 |
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