B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles
Summary: Amphitropic proteins and peptides reversibly partition from solution to membrane, a key process that regulates their functions. Experimental approaches classically used to measure protein partitioning into lipid bilayers, such as fluorescence and circular dichroism, are hardly usable when t...
Main Authors: | Mirko Sadi, Nicolas Carvalho, Corentin Léger, Bruno Vitorge, Daniel Ladant, J. Iñaki Guijarro, Alexandre Chenal |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-11-01
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Series: | Cell Reports: Methods |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2667237523002886 |
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