Engineering of Synthetic Transcriptional Switches in Yeast
Transcriptional switches can be utilized for many purposes in synthetic biology, including the assembly of complex genetic circuits to achieve sophisticated cellular systems and the construction of biosensors for real-time monitoring of intracellular metabolite concentrations. Although to date such...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/12/4/557 |
| _version_ | 1797410434465136640 |
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| author | Masahiro Tominaga Akihiko Kondo Jun Ishii |
| author_facet | Masahiro Tominaga Akihiko Kondo Jun Ishii |
| author_sort | Masahiro Tominaga |
| collection | DOAJ |
| description | Transcriptional switches can be utilized for many purposes in synthetic biology, including the assembly of complex genetic circuits to achieve sophisticated cellular systems and the construction of biosensors for real-time monitoring of intracellular metabolite concentrations. Although to date such switches have mainly been developed in prokaryotes, those for eukaryotes are increasingly being reported as both rational and random engineering technologies mature. In this review, we describe yeast transcriptional switches with different modes of action and how to alter their properties. We also discuss directed evolution technologies for the rapid and robust construction of yeast transcriptional switches. |
| first_indexed | 2024-03-09T04:29:57Z |
| format | Article |
| id | doaj.art-98e25f9dc1824c7b9ce0d66be9ff1f64 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T04:29:57Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-98e25f9dc1824c7b9ce0d66be9ff1f642023-12-03T13:37:16ZengMDPI AGLife2075-17292022-04-0112455710.3390/life12040557Engineering of Synthetic Transcriptional Switches in YeastMasahiro Tominaga0Akihiko Kondo1Jun Ishii2Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, JapanEngineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, JapanEngineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, JapanTranscriptional switches can be utilized for many purposes in synthetic biology, including the assembly of complex genetic circuits to achieve sophisticated cellular systems and the construction of biosensors for real-time monitoring of intracellular metabolite concentrations. Although to date such switches have mainly been developed in prokaryotes, those for eukaryotes are increasingly being reported as both rational and random engineering technologies mature. In this review, we describe yeast transcriptional switches with different modes of action and how to alter their properties. We also discuss directed evolution technologies for the rapid and robust construction of yeast transcriptional switches.https://www.mdpi.com/2075-1729/12/4/557genetic switchtranscriptional switchyeastsynthetic transcription factorsynthetic promoterdirected evolution |
| spellingShingle | Masahiro Tominaga Akihiko Kondo Jun Ishii Engineering of Synthetic Transcriptional Switches in Yeast Life genetic switch transcriptional switch yeast synthetic transcription factor synthetic promoter directed evolution |
| title | Engineering of Synthetic Transcriptional Switches in Yeast |
| title_full | Engineering of Synthetic Transcriptional Switches in Yeast |
| title_fullStr | Engineering of Synthetic Transcriptional Switches in Yeast |
| title_full_unstemmed | Engineering of Synthetic Transcriptional Switches in Yeast |
| title_short | Engineering of Synthetic Transcriptional Switches in Yeast |
| title_sort | engineering of synthetic transcriptional switches in yeast |
| topic | genetic switch transcriptional switch yeast synthetic transcription factor synthetic promoter directed evolution |
| url | https://www.mdpi.com/2075-1729/12/4/557 |
| work_keys_str_mv | AT masahirotominaga engineeringofsynthetictranscriptionalswitchesinyeast AT akihikokondo engineeringofsynthetictranscriptionalswitchesinyeast AT junishii engineeringofsynthetictranscriptionalswitchesinyeast |