Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats
Gamma oryzanol (GO) is well known for its antioxidant activity and health-promoting benefits. The gamma oryzanol-loaded zein nanoparticles (GOZNs) were successfully prepared in our previous study. In the present work, the acute oral toxicity of GOZNs was evaluated based on OECD guideline 420. GOZNs...
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| Format: | Article |
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EDP Sciences
2021-01-01
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| Series: | E3S Web of Conferences |
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| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/78/e3sconf_ri2c2021_02011.pdf |
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| author | Rodsuwan Ubonphan Thisayakorn Krittiya Thumthanaruk Benjawan Vatanyoopaisarn Savitri Uttapap Dudsadee Zhong Qixin Rungsardthong Vilai |
| author_facet | Rodsuwan Ubonphan Thisayakorn Krittiya Thumthanaruk Benjawan Vatanyoopaisarn Savitri Uttapap Dudsadee Zhong Qixin Rungsardthong Vilai |
| author_sort | Rodsuwan Ubonphan |
| collection | DOAJ |
| description | Gamma oryzanol (GO) is well known for its antioxidant activity and health-promoting benefits. The gamma oryzanol-loaded zein nanoparticles (GOZNs) were successfully prepared in our previous study. In the present work, the acute oral toxicity of GOZNs was evaluated based on OECD guideline 420. GOZNs were fabricated by the liquid-liquid dispersion and lyophilized. The samples displayed a mean diameter of 311.20±3.01 nm and high loading capacity of 311.22±7.97 to 322.69±5.67 mg-GO/g-powder after 42-day storage at -18oC. Healthy female Sprague Dawley rats (8 weeks of age) were used for the experiments. Five female rats were administered a single dose of 2,000 mg GO/kg body weight via the oral route. Observations of toxicity signs were recorded for the first 24 hours, and the changes in the general physical conditions were monitored for 14 days before the gross necropsy on day 15. The results show that Sprague Dawley rats exhibited normal growth, and neither mortality nor acute toxicity signs were observed throughout the study period. The findings revealed that GOZNs did not have acute toxicity and were safe when administered orally in Sprague Dawley rats for short periods with LD50 > 2,000 mg/kg. |
| first_indexed | 2024-12-15T00:11:55Z |
| format | Article |
| id | doaj.art-98e4abc06c534a8e96a8d53a7cb36e0d |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-15T00:11:55Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-98e4abc06c534a8e96a8d53a7cb36e0d2022-12-21T22:42:33ZengEDP SciencesE3S Web of Conferences2267-12422021-01-013020201110.1051/e3sconf/202130202011e3sconf_ri2c2021_02011Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley ratsRodsuwan Ubonphan0Thisayakorn Krittiya1Thumthanaruk Benjawan2Vatanyoopaisarn Savitri3Uttapap Dudsadee4Zhong Qixin5Rungsardthong Vilai6Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, Food and Agro-Industrial Research Center, King Mongkut’s University of Technology North BangkokExpert Center of Innovative Herbal Products (InnoHerb), Thailand Institute of Scientific and Technological Research (TISTR)Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, Food and Agro-Industrial Research Center, King Mongkut’s University of Technology North BangkokDepartment of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, Food and Agro-Industrial Research Center, King Mongkut’s University of Technology North BangkokDivision of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology ThonburiDepartment of Food Science, The University of TennesseeDepartment of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, Food and Agro-Industrial Research Center, King Mongkut’s University of Technology North BangkokGamma oryzanol (GO) is well known for its antioxidant activity and health-promoting benefits. The gamma oryzanol-loaded zein nanoparticles (GOZNs) were successfully prepared in our previous study. In the present work, the acute oral toxicity of GOZNs was evaluated based on OECD guideline 420. GOZNs were fabricated by the liquid-liquid dispersion and lyophilized. The samples displayed a mean diameter of 311.20±3.01 nm and high loading capacity of 311.22±7.97 to 322.69±5.67 mg-GO/g-powder after 42-day storage at -18oC. Healthy female Sprague Dawley rats (8 weeks of age) were used for the experiments. Five female rats were administered a single dose of 2,000 mg GO/kg body weight via the oral route. Observations of toxicity signs were recorded for the first 24 hours, and the changes in the general physical conditions were monitored for 14 days before the gross necropsy on day 15. The results show that Sprague Dawley rats exhibited normal growth, and neither mortality nor acute toxicity signs were observed throughout the study period. The findings revealed that GOZNs did not have acute toxicity and were safe when administered orally in Sprague Dawley rats for short periods with LD50 > 2,000 mg/kg.https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/78/e3sconf_ri2c2021_02011.pdfgamma oryzanolzein nanocapsulesencapsulationacute oral toxicitysprague dawley rats |
| spellingShingle | Rodsuwan Ubonphan Thisayakorn Krittiya Thumthanaruk Benjawan Vatanyoopaisarn Savitri Uttapap Dudsadee Zhong Qixin Rungsardthong Vilai Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats E3S Web of Conferences gamma oryzanol zein nanocapsules encapsulation acute oral toxicity sprague dawley rats |
| title | Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats |
| title_full | Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats |
| title_fullStr | Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats |
| title_full_unstemmed | Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats |
| title_short | Acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in Sprague Dawley rats |
| title_sort | acute oral toxicity of zein nanoparticles with encapsulated gamma oryzanol in sprague dawley rats |
| topic | gamma oryzanol zein nanocapsules encapsulation acute oral toxicity sprague dawley rats |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/78/e3sconf_ri2c2021_02011.pdf |
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