TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway
Abstract Background Hepatocellular carcinoma is one of the most common and deadly cancers. The aim of this study was to elucidate the role of tRNA methyltransferase 6 (TRMT6) during HCC progression. Methods The role of TRMT6 in the progression and prognosis of HCC was confirmed by analysis of online...
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BMC
2023-01-01
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Series: | European Journal of Medical Research |
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Online Access: | https://doi.org/10.1186/s40001-022-00951-1 |
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author | Yanqing Ye Maosheng Liu Fengfei Wu Shiyu Ou Weidong Wang Jieying Fei Fang Xie Lan Bai |
author_facet | Yanqing Ye Maosheng Liu Fengfei Wu Shiyu Ou Weidong Wang Jieying Fei Fang Xie Lan Bai |
author_sort | Yanqing Ye |
collection | DOAJ |
description | Abstract Background Hepatocellular carcinoma is one of the most common and deadly cancers. The aim of this study was to elucidate the role of tRNA methyltransferase 6 (TRMT6) during HCC progression. Methods The role of TRMT6 in the progression and prognosis of HCC was confirmed by analysis of online databases and clinical human samples. The effects of up-regulation or down-regulation of TRMT6 on HCC cell proliferation and PI3K/AKT pathway-related protein expressions were verified. The molecular mechanism was investigated in vivo by constructing subcutaneous xenograft tumor model. Results TRMT6 was overexpressed in HCC tissues and associated with Tumour-Node-Metastasis (TNM) stage, primary tumor (T) and regional lymph node (N) classification. TRMT6 expressions in HCC cell lines were higher than that in normal liver cell. TRMT6 overexpression can promote HCC cell proliferation, increase the number of S phase cells. Interference with TRMT6 reduced the PI3K/AKT pathway-related protein expressions, and was reversed by the addition of IGF1. Interference with TRMT6 inhibited tumor growth in vivo and was related to PI3K/AKT pathway. Conclusions Overexpression of TRMT6 promote HCC cell proliferation in vivo and in vitro through PI3K/AKT/mTOR axis, which provides a potential choice for the treatment of HCC in clinical practice. |
first_indexed | 2024-04-10T19:44:19Z |
format | Article |
id | doaj.art-98e94aa438e048e9bd1c86e20e510f9d |
institution | Directory Open Access Journal |
issn | 2047-783X |
language | English |
last_indexed | 2024-04-10T19:44:19Z |
publishDate | 2023-01-01 |
publisher | BMC |
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series | European Journal of Medical Research |
spelling | doaj.art-98e94aa438e048e9bd1c86e20e510f9d2023-01-29T12:07:33ZengBMCEuropean Journal of Medical Research2047-783X2023-01-0128111210.1186/s40001-022-00951-1TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathwayYanqing Ye0Maosheng Liu1Fengfei Wu2Shiyu Ou3Weidong Wang4Jieying Fei5Fang Xie6Lan Bai7Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical UniversityDepartment of Gastroenterology, The First Affiliated Hospital of Gannan Medical UniversityGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical UniversityDepartment of Gastroenterology, The Fourth Affiliated Hospital of Guangxi Medical UniversityGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical UniversityGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical UniversityGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical UniversityGuangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical UniversityAbstract Background Hepatocellular carcinoma is one of the most common and deadly cancers. The aim of this study was to elucidate the role of tRNA methyltransferase 6 (TRMT6) during HCC progression. Methods The role of TRMT6 in the progression and prognosis of HCC was confirmed by analysis of online databases and clinical human samples. The effects of up-regulation or down-regulation of TRMT6 on HCC cell proliferation and PI3K/AKT pathway-related protein expressions were verified. The molecular mechanism was investigated in vivo by constructing subcutaneous xenograft tumor model. Results TRMT6 was overexpressed in HCC tissues and associated with Tumour-Node-Metastasis (TNM) stage, primary tumor (T) and regional lymph node (N) classification. TRMT6 expressions in HCC cell lines were higher than that in normal liver cell. TRMT6 overexpression can promote HCC cell proliferation, increase the number of S phase cells. Interference with TRMT6 reduced the PI3K/AKT pathway-related protein expressions, and was reversed by the addition of IGF1. Interference with TRMT6 inhibited tumor growth in vivo and was related to PI3K/AKT pathway. Conclusions Overexpression of TRMT6 promote HCC cell proliferation in vivo and in vitro through PI3K/AKT/mTOR axis, which provides a potential choice for the treatment of HCC in clinical practice.https://doi.org/10.1186/s40001-022-00951-1Hepatocellular carcinomaCell proliferationTRMT6PI3K/AKTmTOR |
spellingShingle | Yanqing Ye Maosheng Liu Fengfei Wu Shiyu Ou Weidong Wang Jieying Fei Fang Xie Lan Bai TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway European Journal of Medical Research Hepatocellular carcinoma Cell proliferation TRMT6 PI3K/AKT mTOR |
title | TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway |
title_full | TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway |
title_fullStr | TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway |
title_full_unstemmed | TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway |
title_short | TRMT6 promotes hepatocellular carcinoma progression through the PI3K/AKT signaling pathway |
title_sort | trmt6 promotes hepatocellular carcinoma progression through the pi3k akt signaling pathway |
topic | Hepatocellular carcinoma Cell proliferation TRMT6 PI3K/AKT mTOR |
url | https://doi.org/10.1186/s40001-022-00951-1 |
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