Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area
Abstract Background The Kato-Katz technique is recommended worldwide for the diagnosis of intestinal schistosomiasis, detecting parasite eggs in feces of infected people. However, new tests have been developed in order to facilitate diagnosis, e.g. by detection of specific antigens secreted by schis...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | Infectious Diseases of Poverty |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40249-019-0551-7 |
| _version_ | 1819021034682056704 |
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| author | Sergei Rodrigo Magalhães de Sousa Isabelle Helena Lima Dias Álvaro Luan Santana Fonseca Bianca Rodrigues Contente Joyce Favacho Cardoso Nogueira Tatyellen Natasha da Costa Oliveira Stefan Michael Geiger Martin Johannes Enk |
| author_facet | Sergei Rodrigo Magalhães de Sousa Isabelle Helena Lima Dias Álvaro Luan Santana Fonseca Bianca Rodrigues Contente Joyce Favacho Cardoso Nogueira Tatyellen Natasha da Costa Oliveira Stefan Michael Geiger Martin Johannes Enk |
| author_sort | Sergei Rodrigo Magalhães de Sousa |
| collection | DOAJ |
| description | Abstract Background The Kato-Katz technique is recommended worldwide for the diagnosis of intestinal schistosomiasis, detecting parasite eggs in feces of infected people. However, new tests have been developed in order to facilitate diagnosis, e.g. by detection of specific antigens secreted by schistosomes, such as the circulating cathodic antigen (CCA). The aim of this study was to evaluate the performance of the point-of-care circulating cathodic antigen test (POC-CCA) compared to the Kato-Katz technique in a low prevalence area in the Amazon Region, located in the municipality of Primavera, State of Pará, Brazil. Methods Positivity rates of the POC-CCA test and the Kato-Katz technique were calculated. The sensitivity, specificity, accuracy and kappa coefficient were determined by comparing both methods. The reference standard was established using 16 Kato-Katz slides, 12 of the first fecal sample, two of the second and two of the third one. The study also included the concordance between POC-CCA results and different numbers and combinations of Kato-Katz slides. Results The prevalence of schistosomiasis according to the reference standard or POC-CCA test reached a rate of 9.4% or 23.9%, respectively, among a total of 372 participants. The positivity rates by the Kato-Katz technique increased from 2.4 to 9.4%, according to the increase in the number of slides examined and fecal samples collected. A sensitivity of 55.6%, specificity 76.9%, accuracy 76% and κ coefficient of 0.06 was observed by comparing one slide of the first sample and POC-CCA. Comparing 6 slides from three different samples, two slides of each, with POC-CCA resulted in a sensitivity of 58.3%, specificity 78.4%, accuracy 77% and κ coefficient of 0.16. Finally, the comparison of 16 slides from three different samples with POC-CCA revealed a sensitivity of 65.7%, specificity 80.4%, accuracy 79%, and κ coefficient of 0.27. Conclusions The immunochromatographic test has the potential to be an important tool to combat schistosomiasis because of its practicality and applicability but should be applied with caution in low prevalence areas and in programs that aim to eliminate this disease. Trial registration CAAE#21824513.9.0000.5091. January 31st, 2014. |
| first_indexed | 2024-12-21T04:00:41Z |
| format | Article |
| id | doaj.art-98ec051d5f19473c8df34c35953d9b63 |
| institution | Directory Open Access Journal |
| issn | 2049-9957 |
| language | English |
| last_indexed | 2024-12-21T04:00:41Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
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| series | Infectious Diseases of Poverty |
| spelling | doaj.art-98ec051d5f19473c8df34c35953d9b632022-12-21T19:16:44ZengBMCInfectious Diseases of Poverty2049-99572019-05-01811910.1186/s40249-019-0551-7Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity areaSergei Rodrigo Magalhães de Sousa0Isabelle Helena Lima Dias1Álvaro Luan Santana Fonseca2Bianca Rodrigues Contente3Joyce Favacho Cardoso Nogueira4Tatyellen Natasha da Costa Oliveira5Stefan Michael Geiger6Martin Johannes Enk7Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do ParáPrograma de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do ParáPrograma de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do ParáPrograma de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do ParáPrograma de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do ParáPrograma de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do ParáDepartamento de Parasitologia, Universidade Federal de Minas GeraisPrograma de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do ParáAbstract Background The Kato-Katz technique is recommended worldwide for the diagnosis of intestinal schistosomiasis, detecting parasite eggs in feces of infected people. However, new tests have been developed in order to facilitate diagnosis, e.g. by detection of specific antigens secreted by schistosomes, such as the circulating cathodic antigen (CCA). The aim of this study was to evaluate the performance of the point-of-care circulating cathodic antigen test (POC-CCA) compared to the Kato-Katz technique in a low prevalence area in the Amazon Region, located in the municipality of Primavera, State of Pará, Brazil. Methods Positivity rates of the POC-CCA test and the Kato-Katz technique were calculated. The sensitivity, specificity, accuracy and kappa coefficient were determined by comparing both methods. The reference standard was established using 16 Kato-Katz slides, 12 of the first fecal sample, two of the second and two of the third one. The study also included the concordance between POC-CCA results and different numbers and combinations of Kato-Katz slides. Results The prevalence of schistosomiasis according to the reference standard or POC-CCA test reached a rate of 9.4% or 23.9%, respectively, among a total of 372 participants. The positivity rates by the Kato-Katz technique increased from 2.4 to 9.4%, according to the increase in the number of slides examined and fecal samples collected. A sensitivity of 55.6%, specificity 76.9%, accuracy 76% and κ coefficient of 0.06 was observed by comparing one slide of the first sample and POC-CCA. Comparing 6 slides from three different samples, two slides of each, with POC-CCA resulted in a sensitivity of 58.3%, specificity 78.4%, accuracy 77% and κ coefficient of 0.16. Finally, the comparison of 16 slides from three different samples with POC-CCA revealed a sensitivity of 65.7%, specificity 80.4%, accuracy 79%, and κ coefficient of 0.27. Conclusions The immunochromatographic test has the potential to be an important tool to combat schistosomiasis because of its practicality and applicability but should be applied with caution in low prevalence areas and in programs that aim to eliminate this disease. Trial registration CAAE#21824513.9.0000.5091. January 31st, 2014.http://link.springer.com/article/10.1186/s40249-019-0551-7Schistosoma mansoniKato-KatzPOC-CCAAccuracyDiagnosisLow prevalence area |
| spellingShingle | Sergei Rodrigo Magalhães de Sousa Isabelle Helena Lima Dias Álvaro Luan Santana Fonseca Bianca Rodrigues Contente Joyce Favacho Cardoso Nogueira Tatyellen Natasha da Costa Oliveira Stefan Michael Geiger Martin Johannes Enk Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area Infectious Diseases of Poverty Schistosoma mansoni Kato-Katz POC-CCA Accuracy Diagnosis Low prevalence area |
| title | Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area |
| title_full | Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area |
| title_fullStr | Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area |
| title_full_unstemmed | Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area |
| title_short | Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area |
| title_sort | concordance of the point of care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area |
| topic | Schistosoma mansoni Kato-Katz POC-CCA Accuracy Diagnosis Low prevalence area |
| url | http://link.springer.com/article/10.1186/s40249-019-0551-7 |
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