Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.
Cells have a regulatory mechanism known as heat shock (HS) response, which induces the expression of HS genes and proteins in response to heat and other cellular stresses. Exposure to moderate HS results in beneficial effects, such as thermotolerance and promotes survival, whereas excessive HS cause...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0249954 |
| _version_ | 1818929556371800064 |
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| author | Hiroyuki Omori Masahiro Otsu Haruo Nogami Masayoshi Shibata |
| author_facet | Hiroyuki Omori Masahiro Otsu Haruo Nogami Masayoshi Shibata |
| author_sort | Hiroyuki Omori |
| collection | DOAJ |
| description | Cells have a regulatory mechanism known as heat shock (HS) response, which induces the expression of HS genes and proteins in response to heat and other cellular stresses. Exposure to moderate HS results in beneficial effects, such as thermotolerance and promotes survival, whereas excessive HS causes cell death. The effect of HS on cells depends on both exogenous factors, including the temperature and duration of heat application, and endogenous factors, such as the degree of cell differentiation. Neural stem cells (NSCs) can self-renew and differentiate into neurons and glial cells, but the changes in the HS response of symmetrically proliferating NSCs in culture are unclear. We evaluated the HS response of homogeneous proliferating NSCs derived from mouse embryonic stem cells during the proliferative phase and its effect on survival and cell death in vitro. The number of adherent cells and the expression ratios of HS protein (Hsp)40 and Hsp70 genes after exposure to HS for 20 min at temperatures above 43°C significantly increased with the extension of the culture period before exposure to HS. In contrast, caspase activity was significantly decreased by extension of the culture period before exposure to HS and suppressed the decrease in cell viability. These results suggest that the culture period before HS remarkably affects the HS response, influencing the expression of HS genes and cell survival of proliferating NSCs in culture. |
| first_indexed | 2024-12-20T03:46:41Z |
| format | Article |
| id | doaj.art-98edc173140d418e8937ee07c7e931ef |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:46:41Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-98edc173140d418e8937ee07c7e931ef2022-12-21T19:54:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01164e024995410.1371/journal.pone.0249954Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.Hiroyuki OmoriMasahiro OtsuHaruo NogamiMasayoshi ShibataCells have a regulatory mechanism known as heat shock (HS) response, which induces the expression of HS genes and proteins in response to heat and other cellular stresses. Exposure to moderate HS results in beneficial effects, such as thermotolerance and promotes survival, whereas excessive HS causes cell death. The effect of HS on cells depends on both exogenous factors, including the temperature and duration of heat application, and endogenous factors, such as the degree of cell differentiation. Neural stem cells (NSCs) can self-renew and differentiate into neurons and glial cells, but the changes in the HS response of symmetrically proliferating NSCs in culture are unclear. We evaluated the HS response of homogeneous proliferating NSCs derived from mouse embryonic stem cells during the proliferative phase and its effect on survival and cell death in vitro. The number of adherent cells and the expression ratios of HS protein (Hsp)40 and Hsp70 genes after exposure to HS for 20 min at temperatures above 43°C significantly increased with the extension of the culture period before exposure to HS. In contrast, caspase activity was significantly decreased by extension of the culture period before exposure to HS and suppressed the decrease in cell viability. These results suggest that the culture period before HS remarkably affects the HS response, influencing the expression of HS genes and cell survival of proliferating NSCs in culture.https://doi.org/10.1371/journal.pone.0249954 |
| spellingShingle | Hiroyuki Omori Masahiro Otsu Haruo Nogami Masayoshi Shibata Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells. PLoS ONE |
| title | Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells. |
| title_full | Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells. |
| title_fullStr | Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells. |
| title_full_unstemmed | Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells. |
| title_short | Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells. |
| title_sort | heat shock response enhanced by cell culture treatment in mouse embryonic stem cell derived proliferating neural stem cells |
| url | https://doi.org/10.1371/journal.pone.0249954 |
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