A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability
In the present study, polysaccharides from the fruits of Cudrania tricuspidata were purified hierarchically using DEAE-52 cellulose columns and Sephadex S-100 gel columns. Two fractions were obtained and named ZGs-1 and ZGs-2. The in vitro antioxidant and alcohol dehydrogenase activities of ZGs-1 an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623004504 |
| _version_ | 1797632711656996864 |
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| author | Zikang Ding Meng Zhao Xinyue Li Xiaomei Wang Zhongshan Zhang |
| author_facet | Zikang Ding Meng Zhao Xinyue Li Xiaomei Wang Zhongshan Zhang |
| author_sort | Zikang Ding |
| collection | DOAJ |
| description | In the present study, polysaccharides from the fruits of Cudrania tricuspidata were purified hierarchically using DEAE-52 cellulose columns and Sephadex S-100 gel columns. Two fractions were obtained and named ZGs-1 and ZGs-2. The in vitro antioxidant and alcohol dehydrogenase activities of ZGs-1 and ZGs-2 were also analyzed. Two grades named ZGs-1 and ZGs-2 were obtained with molecular weights of 131.521 and 94.551 KDa, respectively, and both consisted of arabinose, galactose, glucose and small amounts of xylose and rhamnose. The structural resolution of these two levels by FT-IR, methylation and NMR showed that the major sugar residue types of ZGs-1 are: →3)-β-D-Galp-(1→, α-L-Araf-(1→, →3,6)-β-D-Galp-(1→, β-D-Xylp-(1→, →5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glc-(1→. The main sugar residue types of ZGs-2 are: →6)-β-D-Galp-(1→, →5)-α-L-Araf-(1→, →3,6)-β-D-Galp-(1→, →3,5)-α-L-Araf-(1→, →4)-β-D-Glc-(1→, α-L-Araf-(1→. ZGs-1 showed weak antioxidant activity. ZGs-2 showed good scavenging effects on DPPH and hydroxyl radicals, as well as on iron ion reduction ability, and the activation rate of alcohol dehydrogenase reached 92.15%, indicating that the novel polysaccharide ZGs-2 is a potential antioxidant and hepatoprotective agent. |
| first_indexed | 2024-03-11T11:41:41Z |
| format | Article |
| id | doaj.art-98f84a7c0a7d4ebc8bddc7ae533fb894 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-11T11:41:41Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-98f84a7c0a7d4ebc8bddc7ae533fb8942023-11-10T04:16:12ZengElsevierJournal of Functional Foods1756-46462023-11-01110105850A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating abilityZikang Ding0Meng Zhao1Xinyue Li2Xiaomei Wang3Zhongshan Zhang4Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, ChinaKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, ChinaKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, ChinaKey Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, China; Corresponding authors at: Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, China (Z. Zhang).Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, China; Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, Hainan Normal University, Haikou 570100, China; Corresponding authors at: Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou 313000, China (Z. Zhang).In the present study, polysaccharides from the fruits of Cudrania tricuspidata were purified hierarchically using DEAE-52 cellulose columns and Sephadex S-100 gel columns. Two fractions were obtained and named ZGs-1 and ZGs-2. The in vitro antioxidant and alcohol dehydrogenase activities of ZGs-1 and ZGs-2 were also analyzed. Two grades named ZGs-1 and ZGs-2 were obtained with molecular weights of 131.521 and 94.551 KDa, respectively, and both consisted of arabinose, galactose, glucose and small amounts of xylose and rhamnose. The structural resolution of these two levels by FT-IR, methylation and NMR showed that the major sugar residue types of ZGs-1 are: →3)-β-D-Galp-(1→, α-L-Araf-(1→, →3,6)-β-D-Galp-(1→, β-D-Xylp-(1→, →5)-α-L-Araf-(1→, →3,5)-α-L-Araf-(1→, →4)-α-D-Glc-(1→. The main sugar residue types of ZGs-2 are: →6)-β-D-Galp-(1→, →5)-α-L-Araf-(1→, →3,6)-β-D-Galp-(1→, →3,5)-α-L-Araf-(1→, →4)-β-D-Glc-(1→, α-L-Araf-(1→. ZGs-1 showed weak antioxidant activity. ZGs-2 showed good scavenging effects on DPPH and hydroxyl radicals, as well as on iron ion reduction ability, and the activation rate of alcohol dehydrogenase reached 92.15%, indicating that the novel polysaccharide ZGs-2 is a potential antioxidant and hepatoprotective agent.http://www.sciencedirect.com/science/article/pii/S1756464623004504Cudrania tricuspidataPolysaccharidesStructure characterizationAntioxidant activityAlcohol dehydrogenase activity |
| spellingShingle | Zikang Ding Meng Zhao Xinyue Li Xiaomei Wang Zhongshan Zhang A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability Journal of Functional Foods Cudrania tricuspidata Polysaccharides Structure characterization Antioxidant activity Alcohol dehydrogenase activity |
| title | A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability |
| title_full | A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability |
| title_fullStr | A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability |
| title_full_unstemmed | A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability |
| title_short | A novel polysaccharide from the fruits of Cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability |
| title_sort | novel polysaccharide from the fruits of cudrania tricuspidata and its antioxidant and alcohol dehydrogenase activating ability |
| topic | Cudrania tricuspidata Polysaccharides Structure characterization Antioxidant activity Alcohol dehydrogenase activity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623004504 |
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