Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac

Introduction: Varnish fluoride has the ability to prevent enamel demineralization. De-waxed shellac that mostly used for fruits coating, now is applied as varnish fluoride materials. This de-waxed shellac is quite potential since the product can be found from Indonesian sources. The research aims to...

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Main Authors: Nina Djustiana, Veni Takarini, Zulia Hasratiningsih
Format: Article
Language:English
Published: Universitas Padjadjaran 2019-07-01
Series:Padjadjaran Journal of Dentistry
Subjects:
Online Access:http://jurnal.unpad.ac.id/pjd/article/view/22657
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author Nina Djustiana
Veni Takarini
Zulia Hasratiningsih
author_facet Nina Djustiana
Veni Takarini
Zulia Hasratiningsih
author_sort Nina Djustiana
collection DOAJ
description Introduction: Varnish fluoride has the ability to prevent enamel demineralization. De-waxed shellac that mostly used for fruits coating, now is applied as varnish fluoride materials. This de-waxed shellac is quite potential since the product can be found from Indonesian sources. The research aims to describe the toxicity effect of varnish fluoride made of de-waxed shellac by measuring the viability of the fibroblast cells. Methods: The research method that was used in this study is in vitro experimental method, which using the tooth that had been smeared with varnish fluoride contacted with fibroblast cells REF (Rat Embryonic Fibroblast) for 4, 8, and 24 hours. The toxicity effect of varnish fluoride was then measured by cell viability. Results The results show that the average cells viability on 4 hours contacted tooth was 88%, while average cells viability on 8 hours contacted tooth was 74%, and lastly the average cells viability on 24 hours contacted tooth was 63%. Conclusion: The conclusion of this preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac are different based on contacted time. Keywords: Cell viability, toxicity effect, varnish fluoride, Indonesian de-waxed shellac
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spelling doaj.art-98f875b47e7441ad83561caa05ce481c2022-12-21T21:59:51ZengUniversitas PadjadjaranPadjadjaran Journal of Dentistry1979-02012549-62122019-07-0131213614110.24198/pjd.vol31no2.2265711299Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellacNina Djustiana0Veni Takarini1Zulia Hasratiningsih2Universitas PadjadjaranUniversitas PadjadjaranUniversitas PadjadjaranIntroduction: Varnish fluoride has the ability to prevent enamel demineralization. De-waxed shellac that mostly used for fruits coating, now is applied as varnish fluoride materials. This de-waxed shellac is quite potential since the product can be found from Indonesian sources. The research aims to describe the toxicity effect of varnish fluoride made of de-waxed shellac by measuring the viability of the fibroblast cells. Methods: The research method that was used in this study is in vitro experimental method, which using the tooth that had been smeared with varnish fluoride contacted with fibroblast cells REF (Rat Embryonic Fibroblast) for 4, 8, and 24 hours. The toxicity effect of varnish fluoride was then measured by cell viability. Results The results show that the average cells viability on 4 hours contacted tooth was 88%, while average cells viability on 8 hours contacted tooth was 74%, and lastly the average cells viability on 24 hours contacted tooth was 63%. Conclusion: The conclusion of this preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac are different based on contacted time. Keywords: Cell viability, toxicity effect, varnish fluoride, Indonesian de-waxed shellachttp://jurnal.unpad.ac.id/pjd/article/view/22657cell viability, toxicity effect, varnish fluoride, indonesian de-waxed shellac
spellingShingle Nina Djustiana
Veni Takarini
Zulia Hasratiningsih
Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac
Padjadjaran Journal of Dentistry
cell viability, toxicity effect, varnish fluoride, indonesian de-waxed shellac
title Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac
title_full Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac
title_fullStr Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac
title_full_unstemmed Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac
title_short Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac
title_sort preliminary study on cells viability based on toxicity effect of varnish fluoride made from indonesian de waxed shellac
topic cell viability, toxicity effect, varnish fluoride, indonesian de-waxed shellac
url http://jurnal.unpad.ac.id/pjd/article/view/22657
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AT venitakarini preliminarystudyoncellsviabilitybasedontoxicityeffectofvarnishfluoridemadefromindonesiandewaxedshellac
AT zuliahasratiningsih preliminarystudyoncellsviabilitybasedontoxicityeffectofvarnishfluoridemadefromindonesiandewaxedshellac