<i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern
During a prokaryotic diversity study in Añana Salt Valley, a new <i>Rhodobacteraceae</i> member, designated ASV31<sup>T</sup>, was isolated from Santa Engracia spring water. It was extremely halotolerant, tolerating up to 23% NaCl, and facultatively alkaliphilic, growing at p...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/1424-2818/14/11/1009 |
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| author | Maia Azpiazu-Muniozguren Minerva García Lorena Laorden Irati Martinez-Malaxetxebarria Sergio Seoane Joseba Bikandi Javier Garaizar Ilargi Martínez-Ballesteros |
| author_facet | Maia Azpiazu-Muniozguren Minerva García Lorena Laorden Irati Martinez-Malaxetxebarria Sergio Seoane Joseba Bikandi Javier Garaizar Ilargi Martínez-Ballesteros |
| author_sort | Maia Azpiazu-Muniozguren |
| collection | DOAJ |
| description | During a prokaryotic diversity study in Añana Salt Valley, a new <i>Rhodobacteraceae</i> member, designated ASV31<sup>T</sup>, was isolated from Santa Engracia spring water. It was extremely halotolerant, tolerating up to 23% NaCl, and facultatively alkaliphilic, growing at pH 6.5–9.5 (optimum at 7.0–9.5). The isolate was a Gram-negative, rod-shaped, aerobic and non-motile bacterium that formed beige-to-pink colonies on marine agar. According to a 16S rRNA gene-based phylogenetic analysis, strain ASV31<sup>T</sup> forms a distinct branch of the family <i>Rhodobacteraceae</i>, with <i>Thioclava pacifica</i> DSM 10166<sup>T</sup> being its closest type strain (95.3%). This was confirmed with a phylogenomic tree and the values of ANI (73.9%), dDDH (19.3%), AAI (63.5%) and POCP (56.0%), which were below the genus/species level boundary. Additionally, an ability to degrade aromatic compounds and biosynthesise secondary metabolites was suggested by the genome of strain ASV31<sup>T</sup>. Distinguishing fatty acid profiles and polar lipid content were also observed. The genome size was 3.6 Mbp, with a DNA G+C content of 65.7%. Based on the data obtained, it was considered that strain ASV31<sup>T</sup> (=CECT 30309<sup>T</sup> = LMG 32242<sup>T</sup>) represents a new species of a new genus in the family <i>Rhodobacteraceae</i>, for which the name <i>Anianabacter salinae</i> gen. nov., sp. nov. is proposed. |
| first_indexed | 2024-03-09T18:23:38Z |
| format | Article |
| id | doaj.art-98f945a19c4843e7b3b6edaef9426492 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T18:23:38Z |
| publishDate | 2022-11-01 |
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| series | Diversity |
| spelling | doaj.art-98f945a19c4843e7b3b6edaef94264922023-11-24T08:06:40ZengMDPI AGDiversity1424-28182022-11-011411100910.3390/d14111009<i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental SalternMaia Azpiazu-Muniozguren0Minerva García1Lorena Laorden2Irati Martinez-Malaxetxebarria3Sergio Seoane4Joseba Bikandi5Javier Garaizar6Ilargi Martínez-Ballesteros7MikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Álava, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Álava, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Álava, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Álava, SpainPlant Biology and Ecology Department, Faculty of Science and Technology, University of the Basque Country UPV/EHU, Barrio Sarriena s/n, 48940 Leioa, Bizkaia, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Álava, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Álava, SpainMikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Álava, SpainDuring a prokaryotic diversity study in Añana Salt Valley, a new <i>Rhodobacteraceae</i> member, designated ASV31<sup>T</sup>, was isolated from Santa Engracia spring water. It was extremely halotolerant, tolerating up to 23% NaCl, and facultatively alkaliphilic, growing at pH 6.5–9.5 (optimum at 7.0–9.5). The isolate was a Gram-negative, rod-shaped, aerobic and non-motile bacterium that formed beige-to-pink colonies on marine agar. According to a 16S rRNA gene-based phylogenetic analysis, strain ASV31<sup>T</sup> forms a distinct branch of the family <i>Rhodobacteraceae</i>, with <i>Thioclava pacifica</i> DSM 10166<sup>T</sup> being its closest type strain (95.3%). This was confirmed with a phylogenomic tree and the values of ANI (73.9%), dDDH (19.3%), AAI (63.5%) and POCP (56.0%), which were below the genus/species level boundary. Additionally, an ability to degrade aromatic compounds and biosynthesise secondary metabolites was suggested by the genome of strain ASV31<sup>T</sup>. Distinguishing fatty acid profiles and polar lipid content were also observed. The genome size was 3.6 Mbp, with a DNA G+C content of 65.7%. Based on the data obtained, it was considered that strain ASV31<sup>T</sup> (=CECT 30309<sup>T</sup> = LMG 32242<sup>T</sup>) represents a new species of a new genus in the family <i>Rhodobacteraceae</i>, for which the name <i>Anianabacter salinae</i> gen. nov., sp. nov. is proposed.https://www.mdpi.com/1424-2818/14/11/1009continental salternextremely halotolerant bacteriaalkaliphilic<i>Anianabacter salinae</i>biotechnological applications |
| spellingShingle | Maia Azpiazu-Muniozguren Minerva García Lorena Laorden Irati Martinez-Malaxetxebarria Sergio Seoane Joseba Bikandi Javier Garaizar Ilargi Martínez-Ballesteros <i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern Diversity continental saltern extremely halotolerant bacteria alkaliphilic <i>Anianabacter salinae</i> biotechnological applications |
| title | <i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern |
| title_full | <i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern |
| title_fullStr | <i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern |
| title_full_unstemmed | <i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern |
| title_short | <i>Anianabacter salinae</i> gen. nov., sp. nov. ASV31<sup>T</sup>, a Facultative Alkaliphilic and Extremely Halotolerant Bacterium Isolated from Brine of a Millennial Continental Saltern |
| title_sort | i anianabacter salinae i gen nov sp nov asv31 sup t sup a facultative alkaliphilic and extremely halotolerant bacterium isolated from brine of a millennial continental saltern |
| topic | continental saltern extremely halotolerant bacteria alkaliphilic <i>Anianabacter salinae</i> biotechnological applications |
| url | https://www.mdpi.com/1424-2818/14/11/1009 |
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