BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson
The aim of the work was to determine the optimal conditions for callusogenesis in (in vitro) cultures of ovules, ovaries and anthers from vanishing wild orchid Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson followed by using of the obtained callus tissue to develop micropropagation techniq...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/no3_2015/sheiko_3_2015.pdf |
| _version_ | 1797766867086999552 |
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| author | Sheiko E. A. Kosakivska I. V. |
| author_facet | Sheiko E. A. Kosakivska I. V. |
| author_sort | Sheiko E. A. |
| collection | DOAJ |
| description | The aim of the work was to determine the optimal conditions for callusogenesis in (in vitro) cultures of ovules, ovaries and anthers from vanishing wild orchid Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson followed by using of the obtained callus tissue to develop micropropagation techniques and conservation ex situ. In this study we used biotechnological (cultivation of explants in vitro) and microscopic (making temporary preparations, light microscopy, cytomorphological callus tissue analysis) methods. Nutrient media with optimal concentrations of growth regulators were selected and calluses derived from the generative organs were obtained (we used for ovaries Murashige–Skoog medium that contains 2.5 mg/l 6 of BAP and 1.5 mg/l of 2,4-D; for ovules — nutrient medium Nitsch and Nitsch containing 2.0 mg/l of 6-benzyladenine and 2.5 mg/l of 2,4-D, for the explants from anther — nutrient medium Nitsch and Nitsch containing 3 mg/l 6 of 6- benzyladenine and 2.5 mg/l of IBA). Cytomorphological analysis reveals the presence of meristematic foci in calluses. It indicates the beginning of the secondary processes of differentiation in the callus tissue. The results may be used in further investigations to obtain O. sphegodes regenerated plants from callus. |
| first_indexed | 2024-03-12T20:31:28Z |
| format | Article |
| id | doaj.art-98fc5f18e38e46148eb7de0dd0d795bb |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T20:31:28Z |
| publishDate | 2015-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-98fc5f18e38e46148eb7de0dd0d795bb2023-08-02T00:01:17ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2015-08-018311612210.15407/biotech8.03.116BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. NelsonSheiko E. A.0 Kosakivska I. V.1Institute of Botany of the National Academy of Sciences of Ukraine, KyivInstitute of Botany of the National Academy of Sciences of Ukraine, KyivThe aim of the work was to determine the optimal conditions for callusogenesis in (in vitro) cultures of ovules, ovaries and anthers from vanishing wild orchid Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson followed by using of the obtained callus tissue to develop micropropagation techniques and conservation ex situ. In this study we used biotechnological (cultivation of explants in vitro) and microscopic (making temporary preparations, light microscopy, cytomorphological callus tissue analysis) methods. Nutrient media with optimal concentrations of growth regulators were selected and calluses derived from the generative organs were obtained (we used for ovaries Murashige–Skoog medium that contains 2.5 mg/l 6 of BAP and 1.5 mg/l of 2,4-D; for ovules — nutrient medium Nitsch and Nitsch containing 2.0 mg/l of 6-benzyladenine and 2.5 mg/l of 2,4-D, for the explants from anther — nutrient medium Nitsch and Nitsch containing 3 mg/l 6 of 6- benzyladenine and 2.5 mg/l of IBA). Cytomorphological analysis reveals the presence of meristematic foci in calluses. It indicates the beginning of the secondary processes of differentiation in the callus tissue. The results may be used in further investigations to obtain O. sphegodes regenerated plants from callus.http://biotechnology.kiev.ua/images/no3_2015/sheiko_3_2015.pdfOphrys sphegodesin vitro culturecallusphytohormones |
| spellingShingle | Sheiko E. A. Kosakivska I. V. BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson Biotechnologia Acta Ophrys sphegodes in vitro culture callus phytohormones |
| title | BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson |
| title_full | BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson |
| title_fullStr | BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson |
| title_full_unstemmed | BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson |
| title_short | BIOTECHNOLOGY OF PRESERVATION OF THE RARE WILD ORCHID OF UKRAINIAN FLORA Ophrys sphegodes subsp. mammosa (Desf.) Soó ex E. Nelson |
| title_sort | biotechnology of preservation of the rare wild orchid of ukrainian flora ophrys sphegodes subsp mammosa desf soo ex e nelson |
| topic | Ophrys sphegodes in vitro culture callus phytohormones |
| url | http://biotechnology.kiev.ua/images/no3_2015/sheiko_3_2015.pdf |
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