Denaturation process of laccase in various media by refractive index measurements
In this work, we are interested in the denaturation process of a laccase from Tramates versicolor via the determination of the refractive index, the refractive index increment and the specific volume in various media. The measurements were carried out using an Abbe refractometer. We have shown that...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580817301280 |
| _version_ | 1819059588897439744 |
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| author | O. Saoudi N. Ghaouar S. Ben Salah T. Othman |
| author_facet | O. Saoudi N. Ghaouar S. Ben Salah T. Othman |
| author_sort | O. Saoudi |
| collection | DOAJ |
| description | In this work, we are interested in the denaturation process of a laccase from Tramates versicolor via the determination of the refractive index, the refractive index increment and the specific volume in various media. The measurements were carried out using an Abbe refractometer. We have shown that the refractive index increment values obtained from the slope of the variation of the refractive index vs. Concentration are outside the range refractive index increments of proteins. To correct the results, we have followed the theoretical predictions based on the knowledge of the protein refractive index from its amino acids composition. The denaturation process was studied by calculating the specific volume variation where its determination was related to the Gladstone-Dale and the Lorentz-Lorentz models. |
| first_indexed | 2024-12-21T14:13:29Z |
| format | Article |
| id | doaj.art-98ff5ff64a454224a3fe22ec4d34f256 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-21T14:13:29Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-98ff5ff64a454224a3fe22ec4d34f2562022-12-21T19:00:59ZengElsevierBiochemistry and Biophysics Reports2405-58082017-09-0111C192610.1016/j.bbrep.2017.05.005Denaturation process of laccase in various media by refractive index measurementsO. Saoudi0N. Ghaouar1S. Ben Salah2T. Othman3Université de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Physique de la Matière Molle et de la Modélisation Electromagnétique, 2092 Tunis, TunisiaUniversité de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Physique de la Matière Molle et de la Modélisation Electromagnétique, 2092 Tunis, TunisiaUniversité de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Physique de la Matière Molle et de la Modélisation Electromagnétique, 2092 Tunis, TunisiaUniversité de Tunis El Manar, Faculté des Sciences de Tunis, Laboratoire de Physique de la Matière Molle et de la Modélisation Electromagnétique, 2092 Tunis, TunisiaIn this work, we are interested in the denaturation process of a laccase from Tramates versicolor via the determination of the refractive index, the refractive index increment and the specific volume in various media. The measurements were carried out using an Abbe refractometer. We have shown that the refractive index increment values obtained from the slope of the variation of the refractive index vs. Concentration are outside the range refractive index increments of proteins. To correct the results, we have followed the theoretical predictions based on the knowledge of the protein refractive index from its amino acids composition. The denaturation process was studied by calculating the specific volume variation where its determination was related to the Gladstone-Dale and the Lorentz-Lorentz models.http://www.sciencedirect.com/science/article/pii/S2405580817301280LaccaseRefractive index incrementSpecific volumeDenaturation |
| spellingShingle | O. Saoudi N. Ghaouar S. Ben Salah T. Othman Denaturation process of laccase in various media by refractive index measurements Biochemistry and Biophysics Reports Laccase Refractive index increment Specific volume Denaturation |
| title | Denaturation process of laccase in various media by refractive index measurements |
| title_full | Denaturation process of laccase in various media by refractive index measurements |
| title_fullStr | Denaturation process of laccase in various media by refractive index measurements |
| title_full_unstemmed | Denaturation process of laccase in various media by refractive index measurements |
| title_short | Denaturation process of laccase in various media by refractive index measurements |
| title_sort | denaturation process of laccase in various media by refractive index measurements |
| topic | Laccase Refractive index increment Specific volume Denaturation |
| url | http://www.sciencedirect.com/science/article/pii/S2405580817301280 |
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