Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat Stroke
Heat stroke (HS) is a severe condition characterized by increased morbidity and high mortality. Acute liver injury (ALI) is a well-documented complication of HS. The tumor suppressor p53 plays an important role in regulation of mitochondrial integrity and mitophagy in several forms of ALI. However,...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.859231/full |
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| author | Wei Huang Wei Huang Weidang Xie Hanhui Zhong Shumin Cai Qiaobing Huang Youtan Liu Zhenhua Zeng Yanan Liu |
| author_facet | Wei Huang Wei Huang Weidang Xie Hanhui Zhong Shumin Cai Qiaobing Huang Youtan Liu Zhenhua Zeng Yanan Liu |
| author_sort | Wei Huang |
| collection | DOAJ |
| description | Heat stroke (HS) is a severe condition characterized by increased morbidity and high mortality. Acute liver injury (ALI) is a well-documented complication of HS. The tumor suppressor p53 plays an important role in regulation of mitochondrial integrity and mitophagy in several forms of ALI. However, the role of p53-regulated mitophagy in HS-ALI remains unclear. In our study, we discovered the dynamic changes of mitophagy in hepatocytes and demonstrated the protective effects of mitophagy activation on HS-ALI. Pretreatment with 3-MA or Mdivi-1 significantly exacerbated ALI by inhibiting mitophagy in HS-ALI mice. Consistent with the animal HS-ALI model results, silencing Parkin aggravated mitochondrial damage and apoptosis by inhibiting mitophagy in HS-treated normal human liver cell line (LO2 cells). Moreover, we described an increase in the translocation of p53 from the nucleus to the cytoplasm, and cytosolic p53 binds to Parkin in LO2 cells following HS. p53 overexpression using a specific adenovirus or Tenovin-6 exacerbated HS-ALI through Parkin-dependent mitophagy both in vivo and in vitro, whereas inhibition of p53 using siRNA or PFT-α effectively reversed this process. Our results demonstrate that cytosolic p53 binds to Parkin and inhibits mitophagy by preventing Parkin’s translocation from the cytosol to the mitochondria, which decreases mitophagy activation and leads to hepatocyte apoptosis in HS-ALI. Overall, pharmacologic induction of mitophagy by inhibiting p53 may be a promising therapeutic approach for HS-ALI treatment. |
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| language | English |
| last_indexed | 2024-04-12T17:22:40Z |
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| series | Frontiers in Immunology |
| spelling | doaj.art-99026db4fc6e41fb8db66c533d33c7192022-12-22T03:23:26ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-05-011310.3389/fimmu.2022.859231859231Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat StrokeWei Huang0Wei Huang1Weidang Xie2Hanhui Zhong3Shumin Cai4Qiaobing Huang5Youtan Liu6Zhenhua Zeng7Yanan Liu8Department of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaThe First School of Clinical Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, ChinaDepartment of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaGuangdong Provincial Key Lab of Shock and Microcirculation, Department of Pathophysiology, Southern Medical University, Guangzhou, ChinaDepartment of Anesthesiology, Shenzhen Hospital, Southern Medical University, Shenzhen, ChinaDepartment of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaHeat stroke (HS) is a severe condition characterized by increased morbidity and high mortality. Acute liver injury (ALI) is a well-documented complication of HS. The tumor suppressor p53 plays an important role in regulation of mitochondrial integrity and mitophagy in several forms of ALI. However, the role of p53-regulated mitophagy in HS-ALI remains unclear. In our study, we discovered the dynamic changes of mitophagy in hepatocytes and demonstrated the protective effects of mitophagy activation on HS-ALI. Pretreatment with 3-MA or Mdivi-1 significantly exacerbated ALI by inhibiting mitophagy in HS-ALI mice. Consistent with the animal HS-ALI model results, silencing Parkin aggravated mitochondrial damage and apoptosis by inhibiting mitophagy in HS-treated normal human liver cell line (LO2 cells). Moreover, we described an increase in the translocation of p53 from the nucleus to the cytoplasm, and cytosolic p53 binds to Parkin in LO2 cells following HS. p53 overexpression using a specific adenovirus or Tenovin-6 exacerbated HS-ALI through Parkin-dependent mitophagy both in vivo and in vitro, whereas inhibition of p53 using siRNA or PFT-α effectively reversed this process. Our results demonstrate that cytosolic p53 binds to Parkin and inhibits mitophagy by preventing Parkin’s translocation from the cytosol to the mitochondria, which decreases mitophagy activation and leads to hepatocyte apoptosis in HS-ALI. Overall, pharmacologic induction of mitophagy by inhibiting p53 may be a promising therapeutic approach for HS-ALI treatment.https://www.frontiersin.org/articles/10.3389/fimmu.2022.859231/fullmitophagyacute liver injuryp53Parkinapoptosis |
| spellingShingle | Wei Huang Wei Huang Weidang Xie Hanhui Zhong Shumin Cai Qiaobing Huang Youtan Liu Zhenhua Zeng Yanan Liu Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat Stroke Frontiers in Immunology mitophagy acute liver injury p53 Parkin apoptosis |
| title | Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat Stroke |
| title_full | Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat Stroke |
| title_fullStr | Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat Stroke |
| title_full_unstemmed | Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat Stroke |
| title_short | Cytosolic p53 Inhibits Parkin-Mediated Mitophagy and Promotes Acute Liver Injury Induced by Heat Stroke |
| title_sort | cytosolic p53 inhibits parkin mediated mitophagy and promotes acute liver injury induced by heat stroke |
| topic | mitophagy acute liver injury p53 Parkin apoptosis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.859231/full |
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