Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons
Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a st...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2013-12-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124713006906 |
| _version_ | 1828425919502483456 |
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| author | Renate Fritzsche Daniela Karra Keiryn L. Bennett Foong yee Ang Jacki E. Heraud-Farlow Marco Tolino Michael Doyle Karl E. Bauer Sabine Thomas Melanie Planyavsky Eric Arn Anetta Bakosova Kerstin Jungwirth Alexandra Hörmann Zsofia Palfi Julia Sandholzer Martina Schwarz Paolo Macchi Jacques Colinge Giulio Superti-Furga Michael A. Kiebler |
| author_facet | Renate Fritzsche Daniela Karra Keiryn L. Bennett Foong yee Ang Jacki E. Heraud-Farlow Marco Tolino Michael Doyle Karl E. Bauer Sabine Thomas Melanie Planyavsky Eric Arn Anetta Bakosova Kerstin Jungwirth Alexandra Hörmann Zsofia Palfi Julia Sandholzer Martina Schwarz Paolo Macchi Jacques Colinge Giulio Superti-Furga Michael A. Kiebler |
| author_sort | Renate Fritzsche |
| collection | DOAJ |
| description | Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to biochemically purify RNA granules from rat brain. Here, we identified a specific set of interactors for two RNA-binding proteins that are known components of neuronal RNA granules, Barentsz and Staufen2. First, neuronal RNA granules are much more heterogeneous than previously anticipated, sharing only a third of the identified proteins. Second, dendritically localized mRNAs, e.g., Arc and CaMKIIα, associate selectively with distinct RNA granules. Third, our work identifies a series of factors with known roles in RNA localization, translational control, and RNA quality control that are likely to keep localized transcripts in a translationally repressed state, often in distinct types of RNPs. |
| first_indexed | 2024-12-10T16:38:33Z |
| format | Article |
| id | doaj.art-9902cfaf8b514a3cb6a5e8da3b5ea0d6 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-10T16:38:33Z |
| publishDate | 2013-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-9902cfaf8b514a3cb6a5e8da3b5ea0d62022-12-22T01:41:18ZengElsevierCell Reports2211-12472013-12-01561749176210.1016/j.celrep.2013.11.023Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in NeuronsRenate Fritzsche0Daniela Karra1Keiryn L. Bennett2Foong yee Ang3Jacki E. Heraud-Farlow4Marco Tolino5Michael Doyle6Karl E. Bauer7Sabine Thomas8Melanie Planyavsky9Eric Arn10Anetta Bakosova11Kerstin Jungwirth12Alexandra Hörmann13Zsofia Palfi14Julia Sandholzer15Martina Schwarz16Paolo Macchi17Jacques Colinge18Giulio Superti-Furga19Michael A. Kiebler20Department of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, AustriaCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, AustriaDepartment of Neuronal Cell Biology, Center for Brain Research, Medical University of Vienna, 1090 Vienna, AustriaTransport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis of specific proteins at active synapses on demand, thereby contributing to learning and memory. To gain insight into the machinery controlling dendritic mRNA localization and translation, we established a stringent protocol to biochemically purify RNA granules from rat brain. Here, we identified a specific set of interactors for two RNA-binding proteins that are known components of neuronal RNA granules, Barentsz and Staufen2. First, neuronal RNA granules are much more heterogeneous than previously anticipated, sharing only a third of the identified proteins. Second, dendritically localized mRNAs, e.g., Arc and CaMKIIα, associate selectively with distinct RNA granules. Third, our work identifies a series of factors with known roles in RNA localization, translational control, and RNA quality control that are likely to keep localized transcripts in a translationally repressed state, often in distinct types of RNPs.http://www.sciencedirect.com/science/article/pii/S2211124713006906 |
| spellingShingle | Renate Fritzsche Daniela Karra Keiryn L. Bennett Foong yee Ang Jacki E. Heraud-Farlow Marco Tolino Michael Doyle Karl E. Bauer Sabine Thomas Melanie Planyavsky Eric Arn Anetta Bakosova Kerstin Jungwirth Alexandra Hörmann Zsofia Palfi Julia Sandholzer Martina Schwarz Paolo Macchi Jacques Colinge Giulio Superti-Furga Michael A. Kiebler Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons Cell Reports |
| title | Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons |
| title_full | Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons |
| title_fullStr | Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons |
| title_full_unstemmed | Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons |
| title_short | Interactome of Two Diverse RNA Granules Links mRNA Localization to Translational Repression in Neurons |
| title_sort | interactome of two diverse rna granules links mrna localization to translational repression in neurons |
| url | http://www.sciencedirect.com/science/article/pii/S2211124713006906 |
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