Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks
Different amino acids have been shown to affect feed intake when injected directly into the central nervous system of birds. In the present study, we investigated the effects of L-glutamine and L-alanine on feed intake and the mRNA expression levels of hypothalamic neuropeptides involved in feed int...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-07-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311912601129 |
| _version_ | 1818838927581118464 |
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| author | Khondowe Paul Song-bo WANG Sheng-feng CHEN Jian-jian YU Xiao-tong ZHU Li-na WANG Ping GAO Qian-yun XI Yong-liang ZHANG Gang SHU Qing-yan JIANG |
| author_facet | Khondowe Paul Song-bo WANG Sheng-feng CHEN Jian-jian YU Xiao-tong ZHU Li-na WANG Ping GAO Qian-yun XI Yong-liang ZHANG Gang SHU Qing-yan JIANG |
| author_sort | Khondowe Paul |
| collection | DOAJ |
| description | Different amino acids have been shown to affect feed intake when injected directly into the central nervous system of birds. In the present study, we investigated the effects of L-glutamine and L-alanine on feed intake and the mRNA expression levels of hypothalamic neuropeptides involved in feed intake regulation in broiler chicks. L-Glutamine or L-alanine was intra-cerebroventricularly (ICV) administered to 4-d-old broiler chicks and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of neuropeptide Y (NPY), agouti related protein (AgRP), pro-opiomelanocortin (POMC), melanocortin receptor 4 (MC4R) and corticotropin releasing factor (CRF). Our results showed that ICV administration of L-glutamine (0.55 or 5.5 μmol) significantly increased feed intake up to 2 h post-administration period and the hypothalamic NPY mRNA expression levels, while it markedly decreased hypothalamic POMC and CRF mRNA expression levels. In contrast, ICV administration of L-alanine (4 μmol) significantly decreased feed intake for the first 0.5 h post-administration period, and reduced the hypothalamic AgRP mRNA expression levels, while it remarkablely enhanced the mRNA expression levels of MC4R and CRF. These findings suggested that L-glutamine and L-alanine could act within the hypothalamus to influence feed intake in broiler chicks, and that both orexigenic and anorexigenic neuropeptide genes might contribute directly to these effects. |
| first_indexed | 2024-12-19T03:46:10Z |
| format | Article |
| id | doaj.art-99036d3409b7445787435c5721bf99ee |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T03:46:10Z |
| publishDate | 2012-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-99036d3409b7445787435c5721bf99ee2022-12-21T20:37:06ZengElsevierJournal of Integrative Agriculture2095-31192012-07-0111711731180Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler ChicksKhondowe Paul0Song-bo WANG1Sheng-feng CHEN2Jian-jian YU3Xiao-tong ZHU4Li-na WANG5Ping GAO6Qian-yun XI7Yong-liang ZHANG8Gang SHU9Qing-yan JIANG10College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. China; School of Natural Sciences, Department of Biological Sciences, University of Zambia, P.O. Box 32379, Lusaka, ZambiaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. ChinaCollege of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. China; SHU Gang, Tel: +86-20-85284901, Fax: +86-20-85284901College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R. China; Correspondence JIANG Qing-yan, Tel: +86-20-85284930, Fax: +86-20-85284901Different amino acids have been shown to affect feed intake when injected directly into the central nervous system of birds. In the present study, we investigated the effects of L-glutamine and L-alanine on feed intake and the mRNA expression levels of hypothalamic neuropeptides involved in feed intake regulation in broiler chicks. L-Glutamine or L-alanine was intra-cerebroventricularly (ICV) administered to 4-d-old broiler chicks and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of neuropeptide Y (NPY), agouti related protein (AgRP), pro-opiomelanocortin (POMC), melanocortin receptor 4 (MC4R) and corticotropin releasing factor (CRF). Our results showed that ICV administration of L-glutamine (0.55 or 5.5 μmol) significantly increased feed intake up to 2 h post-administration period and the hypothalamic NPY mRNA expression levels, while it markedly decreased hypothalamic POMC and CRF mRNA expression levels. In contrast, ICV administration of L-alanine (4 μmol) significantly decreased feed intake for the first 0.5 h post-administration period, and reduced the hypothalamic AgRP mRNA expression levels, while it remarkablely enhanced the mRNA expression levels of MC4R and CRF. These findings suggested that L-glutamine and L-alanine could act within the hypothalamus to influence feed intake in broiler chicks, and that both orexigenic and anorexigenic neuropeptide genes might contribute directly to these effects.http://www.sciencedirect.com/science/article/pii/S2095311912601129L-glutamineL-alanineintracerebroventricular (ICV)feed intakehypothalamus |
| spellingShingle | Khondowe Paul Song-bo WANG Sheng-feng CHEN Jian-jian YU Xiao-tong ZHU Li-na WANG Ping GAO Qian-yun XI Yong-liang ZHANG Gang SHU Qing-yan JIANG Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks Journal of Integrative Agriculture L-glutamine L-alanine intracerebroventricular (ICV) feed intake hypothalamus |
| title | Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks |
| title_full | Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks |
| title_fullStr | Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks |
| title_full_unstemmed | Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks |
| title_short | Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks |
| title_sort | effects of central administration of glutamine and alanine on feed intake and hypothalamic expression of orexigenic and anorexigenic neuropetides in broiler chicks |
| topic | L-glutamine L-alanine intracerebroventricular (ICV) feed intake hypothalamus |
| url | http://www.sciencedirect.com/science/article/pii/S2095311912601129 |
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