Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMI
Background/Aims: Cytosolic glycerol 3-phosphate dehydrogenase (cGPDH) is a key enzyme providing glycerol 3-phosphate for triacylglycerol synthesis in adipose tissue and is regarded as a marker for adipocyte differentiation. The aim of this study was to test the hypothesis that an increase in cGPDH g...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2013-08-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/354438 |
| _version_ | 1819108740808310784 |
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| author | Tomasz Sledzinski Justyna Korczynska Elzbieta Goyke Tomasz Stefaniak Monika Proczko-Markuszewska Lukasz Kaska Julian Swierczynski |
| author_facet | Tomasz Sledzinski Justyna Korczynska Elzbieta Goyke Tomasz Stefaniak Monika Proczko-Markuszewska Lukasz Kaska Julian Swierczynski |
| author_sort | Tomasz Sledzinski |
| collection | DOAJ |
| description | Background/Aims: Cytosolic glycerol 3-phosphate dehydrogenase (cGPDH) is a key enzyme providing glycerol 3-phosphate for triacylglycerol synthesis in adipose tissue and is regarded as a marker for adipocyte differentiation. The aim of this study was to test the hypothesis that an increase in cGPDH gene expression in subcutaneous adipose tissue is associated with obesity. Methods: mRNA levels in human subcutaneous adipose tissue were analysed by Real-Time PCR. Results: We found that human subcutaneous adipose tissue cGPDH activity and cGPDH mRNA level were greater in obese patients than in lean subjects and were positively correlated with BMI and fat mass. Moreover, a strong positive correlation between subcutaneous adipose tissue cGPDH mRNA level and cGPDH activity was found. The data presented here indicates also that PPARγ mRNA level is positively correlated with body mass index and fat mass as well as with adipose tissue cGPDH mRNA level. Moreover, the association between subcutaneous adipose tissue cGPDH mRNA level and fatty acid translocase (FAT/CD36) mRNA level was also observed. Conclusion: The obtained results suggest that in comparison to lean subjects the increase in subcutaneous adipose tissue cGPDH gene expression in the obese, is probably the result of adipose tissue expansion during obesity. |
| first_indexed | 2024-12-22T03:14:44Z |
| format | Article |
| id | doaj.art-990853dd076b4bb185ddcaa88b14712c |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-22T03:14:44Z |
| publishDate | 2013-08-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-990853dd076b4bb185ddcaa88b14712c2022-12-21T18:40:51ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-08-0132230030910.1159/000354438354438Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMITomasz SledzinskiJustyna KorczynskaElzbieta GoykeTomasz StefaniakMonika Proczko-MarkuszewskaLukasz KaskaJulian SwierczynskiBackground/Aims: Cytosolic glycerol 3-phosphate dehydrogenase (cGPDH) is a key enzyme providing glycerol 3-phosphate for triacylglycerol synthesis in adipose tissue and is regarded as a marker for adipocyte differentiation. The aim of this study was to test the hypothesis that an increase in cGPDH gene expression in subcutaneous adipose tissue is associated with obesity. Methods: mRNA levels in human subcutaneous adipose tissue were analysed by Real-Time PCR. Results: We found that human subcutaneous adipose tissue cGPDH activity and cGPDH mRNA level were greater in obese patients than in lean subjects and were positively correlated with BMI and fat mass. Moreover, a strong positive correlation between subcutaneous adipose tissue cGPDH mRNA level and cGPDH activity was found. The data presented here indicates also that PPARγ mRNA level is positively correlated with body mass index and fat mass as well as with adipose tissue cGPDH mRNA level. Moreover, the association between subcutaneous adipose tissue cGPDH mRNA level and fatty acid translocase (FAT/CD36) mRNA level was also observed. Conclusion: The obtained results suggest that in comparison to lean subjects the increase in subcutaneous adipose tissue cGPDH gene expression in the obese, is probably the result of adipose tissue expansion during obesity.http://www.karger.com/Article/FullText/354438Phosphoenolpyruvate carboxykinaseLeptinAdiponectinFAT/CD36ObesityPPARγCytosolic glycerol 3-phosphate dehydrogenaseTriacylglycerol |
| spellingShingle | Tomasz Sledzinski Justyna Korczynska Elzbieta Goyke Tomasz Stefaniak Monika Proczko-Markuszewska Lukasz Kaska Julian Swierczynski Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMI Cellular Physiology and Biochemistry Phosphoenolpyruvate carboxykinase Leptin Adiponectin FAT/CD36 Obesity PPARγ Cytosolic glycerol 3-phosphate dehydrogenase Triacylglycerol |
| title | Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMI |
| title_full | Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMI |
| title_fullStr | Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMI |
| title_full_unstemmed | Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMI |
| title_short | Association Between Cytosolic Glycerol 3-Phosphate Dehydrogenase Gene Expression in Human Subcutaneous Adipose Tissue and BMI |
| title_sort | association between cytosolic glycerol 3 phosphate dehydrogenase gene expression in human subcutaneous adipose tissue and bmi |
| topic | Phosphoenolpyruvate carboxykinase Leptin Adiponectin FAT/CD36 Obesity PPARγ Cytosolic glycerol 3-phosphate dehydrogenase Triacylglycerol |
| url | http://www.karger.com/Article/FullText/354438 |
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