Identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism array
Abstract Renal dysfunction is a long-term complication associated with an increased mortality after lung transplantation (LT). We investigated the association of single-nucleotide polymorphisms (SNPs) with the development of renal dysfunction after LT using a Japanese-specific SNP array. First, elig...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36143-y |
| _version_ | 1827634473562275840 |
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| author | Yasuaki Tomioka Seiichiro Sugimoto Haruchika Yamamoto Shuta Tomida Toshio Shiotani Shin Tanaka Kazuhiko Shien Ken Suzawa Kentaroh Miyoshi Shinji Otani Hiromasa Yamamoto Mikio Okazaki Masaomi Yamane Shinichi Toyooka |
| author_facet | Yasuaki Tomioka Seiichiro Sugimoto Haruchika Yamamoto Shuta Tomida Toshio Shiotani Shin Tanaka Kazuhiko Shien Ken Suzawa Kentaroh Miyoshi Shinji Otani Hiromasa Yamamoto Mikio Okazaki Masaomi Yamane Shinichi Toyooka |
| author_sort | Yasuaki Tomioka |
| collection | DOAJ |
| description | Abstract Renal dysfunction is a long-term complication associated with an increased mortality after lung transplantation (LT). We investigated the association of single-nucleotide polymorphisms (SNPs) with the development of renal dysfunction after LT using a Japanese-specific SNP array. First, eligible samples of 34 LT recipients were genotyped using the SNP array and divided into two groups, according to the presence of homozygous and heterozygous combinations of mutant alleles of the 126 renal-related SNPs. To identify candidate SNPs, the renal function tests were compared between the two groups for each SNP. Next, we investigated the association between the candidate SNPs and the time course of changes of the estimated glomerular filtration rate (eGFR) in the 99 recipients until 10 years after the LT. ΔeGFR was defined as the difference between the postoperative and preoperative eGFR values. Eight SNPs were identified as the candidate SNPs in the 34 recipients. Validation analysis of these 8 candidate SNPs in all the 99 recipients showed that three SNPs, namely, rs10277115, rs4690095, and rs792064, were associated with significant changes of the ΔeGFR. Pre-transplant identification of high-risk patients for the development of renal dysfunction after LT based on the presence of these SNPs might contribute to providing personalized medicine. |
| first_indexed | 2024-03-09T15:13:51Z |
| format | Article |
| id | doaj.art-99096efbdfbb429384a5da8c393e2ceb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:13:51Z |
| publishDate | 2023-06-01 |
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| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-99096efbdfbb429384a5da8c393e2ceb2023-11-26T13:13:05ZengNature PortfolioScientific Reports2045-23222023-06-011311910.1038/s41598-023-36143-yIdentification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism arrayYasuaki Tomioka0Seiichiro Sugimoto1Haruchika Yamamoto2Shuta Tomida3Toshio Shiotani4Shin Tanaka5Kazuhiko Shien6Ken Suzawa7Kentaroh Miyoshi8Shinji Otani9Hiromasa Yamamoto10Mikio Okazaki11Masaomi Yamane12Shinichi Toyooka13Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesCenter for Comprehensive Genomic Medicine, Okayama University HospitalDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesAbstract Renal dysfunction is a long-term complication associated with an increased mortality after lung transplantation (LT). We investigated the association of single-nucleotide polymorphisms (SNPs) with the development of renal dysfunction after LT using a Japanese-specific SNP array. First, eligible samples of 34 LT recipients were genotyped using the SNP array and divided into two groups, according to the presence of homozygous and heterozygous combinations of mutant alleles of the 126 renal-related SNPs. To identify candidate SNPs, the renal function tests were compared between the two groups for each SNP. Next, we investigated the association between the candidate SNPs and the time course of changes of the estimated glomerular filtration rate (eGFR) in the 99 recipients until 10 years after the LT. ΔeGFR was defined as the difference between the postoperative and preoperative eGFR values. Eight SNPs were identified as the candidate SNPs in the 34 recipients. Validation analysis of these 8 candidate SNPs in all the 99 recipients showed that three SNPs, namely, rs10277115, rs4690095, and rs792064, were associated with significant changes of the ΔeGFR. Pre-transplant identification of high-risk patients for the development of renal dysfunction after LT based on the presence of these SNPs might contribute to providing personalized medicine.https://doi.org/10.1038/s41598-023-36143-y |
| spellingShingle | Yasuaki Tomioka Seiichiro Sugimoto Haruchika Yamamoto Shuta Tomida Toshio Shiotani Shin Tanaka Kazuhiko Shien Ken Suzawa Kentaroh Miyoshi Shinji Otani Hiromasa Yamamoto Mikio Okazaki Masaomi Yamane Shinichi Toyooka Identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism array Scientific Reports |
| title | Identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism array |
| title_full | Identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism array |
| title_fullStr | Identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism array |
| title_full_unstemmed | Identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism array |
| title_short | Identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic-specific single-nucleotide polymorphism array |
| title_sort | identification of genetic loci associated with renal dysfunction after lung transplantation using an ethnic specific single nucleotide polymorphism array |
| url | https://doi.org/10.1038/s41598-023-36143-y |
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