High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils
Abstract Background In metabolic disorders, myocardial fatty infiltration is critically associated with lipotoxic cardiomyopathy. Methods Twenty Psammomys obesus gerbils were randomly assigned to normal plant or high fat diet. Sixteen weeks later, myocardium was sampled for pathobiological evaluatio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-020-01301-y |
| _version_ | 1818292642753019904 |
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| author | Abdelhamid Sahraoui Céline Dewachter Grégory Vegh Kathleen Mc Entee Robert Naeije Souhila Aouichat Bouguerra Laurence Dewachter |
| author_facet | Abdelhamid Sahraoui Céline Dewachter Grégory Vegh Kathleen Mc Entee Robert Naeije Souhila Aouichat Bouguerra Laurence Dewachter |
| author_sort | Abdelhamid Sahraoui |
| collection | DOAJ |
| description | Abstract Background In metabolic disorders, myocardial fatty infiltration is critically associated with lipotoxic cardiomyopathy. Methods Twenty Psammomys obesus gerbils were randomly assigned to normal plant or high fat diet. Sixteen weeks later, myocardium was sampled for pathobiological evaluation. Results A sixteen-week high fat diet resulted in myocardial structure disorganization, with collagen deposits, lipid accumulation, cardiomyocyte apoptosis and inflammatory cell infiltration. Myocardial expressions of glucose transporter GLUT1 and pyruvate dehydrogenase (PDH) inhibitor, PDH kinase (PDK)4 increased, while insulin-regulated GLUT4 expression remained unchanged. Myocardial expressions of molecules regulating fatty acid transport, CD36 and fatty acid binding protein (FABP)3, were increased, while expression of rate-controlling fatty acid β-oxidation, carnitine palmitoyl transferase (CPT)1B decreased. Myocardial expression of AMP-activated protein kinase (AMPK), decreased, while expression of peroxisome proliferator activated receptors (PPAR)-α and -γ did not change. Conclusion In high fat diet fed Psammomys obesus, an original experimental model of nutritionally induced metabolic syndrome mixing genetic predisposition and environment interactions, a short period of high fat feeding was sufficient to induce myocardial structural alterations, associated with altered myocardial metabolic gene expression in favor of lipid accumulation. |
| first_indexed | 2024-12-13T03:03:13Z |
| format | Article |
| id | doaj.art-990a7a2830d64e72b6616d7a02e16a99 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-13T03:03:13Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-990a7a2830d64e72b6616d7a02e16a992022-12-22T00:01:47ZengBMCLipids in Health and Disease1476-511X2020-06-0119111110.1186/s12944-020-01301-yHigh fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbilsAbdelhamid Sahraoui0Céline Dewachter1Grégory Vegh2Kathleen Mc Entee3Robert Naeije4Souhila Aouichat Bouguerra5Laurence Dewachter6Laboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de BruxellesLaboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de BruxellesLaboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de BruxellesLaboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de BruxellesLaboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de BruxellesTeam of Cellular and Molecular Physiopathology, Faculty of Biological Sciences, Houari Boumediene University of Sciences and TechnologyLaboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de BruxellesAbstract Background In metabolic disorders, myocardial fatty infiltration is critically associated with lipotoxic cardiomyopathy. Methods Twenty Psammomys obesus gerbils were randomly assigned to normal plant or high fat diet. Sixteen weeks later, myocardium was sampled for pathobiological evaluation. Results A sixteen-week high fat diet resulted in myocardial structure disorganization, with collagen deposits, lipid accumulation, cardiomyocyte apoptosis and inflammatory cell infiltration. Myocardial expressions of glucose transporter GLUT1 and pyruvate dehydrogenase (PDH) inhibitor, PDH kinase (PDK)4 increased, while insulin-regulated GLUT4 expression remained unchanged. Myocardial expressions of molecules regulating fatty acid transport, CD36 and fatty acid binding protein (FABP)3, were increased, while expression of rate-controlling fatty acid β-oxidation, carnitine palmitoyl transferase (CPT)1B decreased. Myocardial expression of AMP-activated protein kinase (AMPK), decreased, while expression of peroxisome proliferator activated receptors (PPAR)-α and -γ did not change. Conclusion In high fat diet fed Psammomys obesus, an original experimental model of nutritionally induced metabolic syndrome mixing genetic predisposition and environment interactions, a short period of high fat feeding was sufficient to induce myocardial structural alterations, associated with altered myocardial metabolic gene expression in favor of lipid accumulation.http://link.springer.com/article/10.1186/s12944-020-01301-yLipotoxicityPsammomys obesusHigh fat dietCardiomyopathyApoptosis |
| spellingShingle | Abdelhamid Sahraoui Céline Dewachter Grégory Vegh Kathleen Mc Entee Robert Naeije Souhila Aouichat Bouguerra Laurence Dewachter High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils Lipids in Health and Disease Lipotoxicity Psammomys obesus High fat diet Cardiomyopathy Apoptosis |
| title | High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils |
| title_full | High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils |
| title_fullStr | High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils |
| title_full_unstemmed | High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils |
| title_short | High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils |
| title_sort | high fat diet altered cardiac metabolic gene profile in psammomys obesus gerbils |
| topic | Lipotoxicity Psammomys obesus High fat diet Cardiomyopathy Apoptosis |
| url | http://link.springer.com/article/10.1186/s12944-020-01301-y |
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