Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro Study
This study evaluated sealing properties of zinc oxide/calcium sulphate filling material (Plastor©) to prevent bacterial infiltration in simulated Class 1 cavities with and without the presence of a spacer apically the restoration. Twenty-eight experimental Eppendorf tubes containing Tryptic Soy Agar...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/3/1748 |
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| author | Alessio Buonavoglia Adriana Trotta Francesco Pellegrini Alfredo Iandolo Marco Cordisco |
| author_facet | Alessio Buonavoglia Adriana Trotta Francesco Pellegrini Alfredo Iandolo Marco Cordisco |
| author_sort | Alessio Buonavoglia |
| collection | DOAJ |
| description | This study evaluated sealing properties of zinc oxide/calcium sulphate filling material (Plastor©) to prevent bacterial infiltration in simulated Class 1 cavities with and without the presence of a spacer apically the restoration. Twenty-eight experimental Eppendorf tubes containing Tryptic Soy Agar were prepared and divided into seven groups: group A (Plastor©), group B (Plastor© + cotton pellet), group C (Plastor© + cotton pellet soaked of m-cresyl acetate), group D (Plastor© + cotton pellet soaked of eugenol), group E (Plastor© + PTFE pellet), group F (positive control), and group G (negative control). All prepared vials were individually immersed in tubes containing 5 mL of a suspension of <i>Streptococcus mutans</i> (10<sup>5</sup> CFU/mL) previously prepared and then incubated at 37 °C for 5 and 10 days. Subsequently, agar was collected and tested for <i>S.mutans</i> using real-time PCR. Aliquots of each agar samples were separately seeded on TSA for <i>S. mutans</i> isolation. The real-time PCR tests were negative for <i>S. mutans</i> on all the vials at both T5 and T10, except for positive control resulted positive at both T5 and T10. The isolation tests showed bacterial growth of <i>S. mutans</i> only with the agar samples collected from the vials of positive control, confirming real-time PCR tests. This in vitro study evidenced complete sealing ability of zinc oxide/calcium sulphate in temporary restorations of Class 1 cavities without mechanical loading at 10 days and complete immersion in a bacterial suspension. Moreover, sealing ability is not influenced by presence of spacers. |
| first_indexed | 2024-03-11T09:52:14Z |
| format | Article |
| id | doaj.art-990b650b0f9740029113f83449e14921 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T09:52:14Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-990b650b0f9740029113f83449e149212023-11-16T16:09:51ZengMDPI AGApplied Sciences2076-34172023-01-01133174810.3390/app13031748Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro StudyAlessio Buonavoglia0Adriana Trotta1Francesco Pellegrini2Alfredo Iandolo3Marco Cordisco4Department of Biomedical and Neuromotor Sciences, School of Dentistry, University of Bologna, 40125 Bologna, ItalyDepartment of Veterinary Medicine, University of Bari “Aldo Moro”, Str. Prov. for Casamassima, Km 3, 70010 Valenzano, ItalyDepartment of Veterinary Medicine, University of Bari “Aldo Moro”, Str. Prov. for Casamassima, Km 3, 70010 Valenzano, ItalyDepartment of Conservative and Endodontics, Faculty of Dentistry, University of Salerno, 84084 Salerno, ItalyDepartment of Veterinary Medicine, University of Bari “Aldo Moro”, Str. Prov. for Casamassima, Km 3, 70010 Valenzano, ItalyThis study evaluated sealing properties of zinc oxide/calcium sulphate filling material (Plastor©) to prevent bacterial infiltration in simulated Class 1 cavities with and without the presence of a spacer apically the restoration. Twenty-eight experimental Eppendorf tubes containing Tryptic Soy Agar were prepared and divided into seven groups: group A (Plastor©), group B (Plastor© + cotton pellet), group C (Plastor© + cotton pellet soaked of m-cresyl acetate), group D (Plastor© + cotton pellet soaked of eugenol), group E (Plastor© + PTFE pellet), group F (positive control), and group G (negative control). All prepared vials were individually immersed in tubes containing 5 mL of a suspension of <i>Streptococcus mutans</i> (10<sup>5</sup> CFU/mL) previously prepared and then incubated at 37 °C for 5 and 10 days. Subsequently, agar was collected and tested for <i>S.mutans</i> using real-time PCR. Aliquots of each agar samples were separately seeded on TSA for <i>S. mutans</i> isolation. The real-time PCR tests were negative for <i>S. mutans</i> on all the vials at both T5 and T10, except for positive control resulted positive at both T5 and T10. The isolation tests showed bacterial growth of <i>S. mutans</i> only with the agar samples collected from the vials of positive control, confirming real-time PCR tests. This in vitro study evidenced complete sealing ability of zinc oxide/calcium sulphate in temporary restorations of Class 1 cavities without mechanical loading at 10 days and complete immersion in a bacterial suspension. Moreover, sealing ability is not influenced by presence of spacers.https://www.mdpi.com/2076-3417/13/3/1748temporary filling materialszinc oxide/calcium sulphate materialsbacterial infiltration<i>Streptococcus mutans</i> |
| spellingShingle | Alessio Buonavoglia Adriana Trotta Francesco Pellegrini Alfredo Iandolo Marco Cordisco Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro Study Applied Sciences temporary filling materials zinc oxide/calcium sulphate materials bacterial infiltration <i>Streptococcus mutans</i> |
| title | Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro Study |
| title_full | Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro Study |
| title_fullStr | Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro Study |
| title_full_unstemmed | Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro Study |
| title_short | Prevention of Bacterial Infiltration in Class 1 Temporary Restorations Using Zinc Oxide/Calcium Sulphate Filling Materials: An In Vitro Study |
| title_sort | prevention of bacterial infiltration in class 1 temporary restorations using zinc oxide calcium sulphate filling materials an in vitro study |
| topic | temporary filling materials zinc oxide/calcium sulphate materials bacterial infiltration <i>Streptococcus mutans</i> |
| url | https://www.mdpi.com/2076-3417/13/3/1748 |
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