Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased Fitness
The number of clinical specimens containing β-lactam-resistant Pseudomonas aeruginosa isolates is increasing. However, whether resistance is associated with reduced fitness is still uncertain in clinical Pseudomonas aeruginosa isolates. In this study, we aimed to determine whether β-lactam resistanc...
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Cell Physiol Biochem Press GmbH & Co KG
2013-03-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/343372 |
| _version_ | 1828856938983587840 |
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| author | Zhenzhen Sun Xiaoyang Jiao Qing Peng Fang Jiang Yuanchun Huang Juan Zhang Fen Yao |
| author_facet | Zhenzhen Sun Xiaoyang Jiao Qing Peng Fang Jiang Yuanchun Huang Juan Zhang Fen Yao |
| author_sort | Zhenzhen Sun |
| collection | DOAJ |
| description | The number of clinical specimens containing β-lactam-resistant Pseudomonas aeruginosa isolates is increasing. However, whether resistance is associated with reduced fitness is still uncertain in clinical Pseudomonas aeruginosa isolates. In this study, we aimed to determine whether β-lactam resistance conferred a fitness cost in Pseudomonas aeruginosa. Growth rate, extracellular slime production, elastase activity, proteolytic activity, LasA protease activity, biofilm formation, and pairwise in vitro competition experiments were investigated in a collection of 11 isogenic, β-lactam-susceptible and -resistant (≥8-fold increase in minimum inhibitory concentration (MIC)) pairs of P. aeruginosa clinical isolates; each pair was recovered from a different patient treated with β-lactam antibiotics. All β-lactam-resistant Pseudomonas aeruginosa isolates showed a significant reduction in elastase activity. In addition, 90.9% (10/11) of β-lactam-resistant Pseudomonas aeruginosa isolates were associated with markedly lower growth rate and proteolytic activity, and 81.8% (9/11) of β-lactam-resistant Pseudomonas aeruginosa isolates had less extracellular slime production, compared to susceptible isolates. Meanwhile, LasA protease activity and biofilm formation ability were variable among isolates. Pairwise in vitro competition experiments showed that 72.7% (8/11) of β- lactam-susceptible strains could outgrow resistant strains. In conclusion, resistance development with β-lactam exposure confers a fitness cost, resulting in a decreased invasion potential, while the effect on viability varied. Thus, the potential for the dissemination of β-lactam-resistant Pseudomonas aeruginosa clinical isolates should not be underestimated. |
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| id | doaj.art-990bcad284e848d8a400989ce9c7f8c4 |
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| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-13T01:26:30Z |
| publishDate | 2013-03-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
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| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-990bcad284e848d8a400989ce9c7f8c42022-12-22T00:04:07ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-03-01312-334735410.1159/000343372343372Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased FitnessZhenzhen SunXiaoyang JiaoQing PengFang JiangYuanchun HuangJuan ZhangFen YaoThe number of clinical specimens containing β-lactam-resistant Pseudomonas aeruginosa isolates is increasing. However, whether resistance is associated with reduced fitness is still uncertain in clinical Pseudomonas aeruginosa isolates. In this study, we aimed to determine whether β-lactam resistance conferred a fitness cost in Pseudomonas aeruginosa. Growth rate, extracellular slime production, elastase activity, proteolytic activity, LasA protease activity, biofilm formation, and pairwise in vitro competition experiments were investigated in a collection of 11 isogenic, β-lactam-susceptible and -resistant (≥8-fold increase in minimum inhibitory concentration (MIC)) pairs of P. aeruginosa clinical isolates; each pair was recovered from a different patient treated with β-lactam antibiotics. All β-lactam-resistant Pseudomonas aeruginosa isolates showed a significant reduction in elastase activity. In addition, 90.9% (10/11) of β-lactam-resistant Pseudomonas aeruginosa isolates were associated with markedly lower growth rate and proteolytic activity, and 81.8% (9/11) of β-lactam-resistant Pseudomonas aeruginosa isolates had less extracellular slime production, compared to susceptible isolates. Meanwhile, LasA protease activity and biofilm formation ability were variable among isolates. Pairwise in vitro competition experiments showed that 72.7% (8/11) of β- lactam-susceptible strains could outgrow resistant strains. In conclusion, resistance development with β-lactam exposure confers a fitness cost, resulting in a decreased invasion potential, while the effect on viability varied. Thus, the potential for the dissemination of β-lactam-resistant Pseudomonas aeruginosa clinical isolates should not be underestimated.http://www.karger.com/Article/FullText/343372Pseudomonas aeruginosaNosocomial infectionBacterial resistanceβ-lactam antibiotics |
| spellingShingle | Zhenzhen Sun Xiaoyang Jiao Qing Peng Fang Jiang Yuanchun Huang Juan Zhang Fen Yao Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased Fitness Cellular Physiology and Biochemistry Pseudomonas aeruginosa Nosocomial infection Bacterial resistance β-lactam antibiotics |
| title | Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased Fitness |
| title_full | Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased Fitness |
| title_fullStr | Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased Fitness |
| title_full_unstemmed | Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased Fitness |
| title_short | Antibiotic Resistance in Pseudomonas Aeruginosa is Associated with Decreased Fitness |
| title_sort | antibiotic resistance in pseudomonas aeruginosa is associated with decreased fitness |
| topic | Pseudomonas aeruginosa Nosocomial infection Bacterial resistance β-lactam antibiotics |
| url | http://www.karger.com/Article/FullText/343372 |
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