Gelsolin-independent podosome formation in dendritic cells.
Podosomes, important structures for adhesion and extracellular matrix degradation, are claimed to be involved in cell migration. In addition, podosomes are also reported to be of importance in tissue remodelling, e.g., in osteoclast-mediated bone resorption. Podosomes are highly dynamic actin-filame...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779330/?tool=EBI |
| _version_ | 1818460308296957952 |
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| author | Oscar Hammarfjord Hervé Falet Christine Gurniak John H Hartwig Robert P A Wallin |
| author_facet | Oscar Hammarfjord Hervé Falet Christine Gurniak John H Hartwig Robert P A Wallin |
| author_sort | Oscar Hammarfjord |
| collection | DOAJ |
| description | Podosomes, important structures for adhesion and extracellular matrix degradation, are claimed to be involved in cell migration. In addition, podosomes are also reported to be of importance in tissue remodelling, e.g., in osteoclast-mediated bone resorption. Podosomes are highly dynamic actin-filament scaffolds onto which proteins important for their function, such as matrix metallo-proteases and integrins, attach. The dynamics of the podosomes require the action of many proteins regulating actin assembly and disassembly. One such protein, gelsolin, which associates to podosomes, has been reported to be important for podosome formation and function in osteoclasts. However, podosome-like structures have been reported in gelsolin-deficient dendritic cells, but the identity of these structures was not confirmed, and their dynamics and function was not investigated. Like many other cells, dendritic cells of the immune system also form matrix degrading podosomes. In the present study, we show that dendritic cells form podosomes independently of gelsolin, that there are no major alterations in their dynamics of formation and disassembly, and that they exhibit matrix-degrading function. Furthermore, we found that gelsolin is not required for TLR4-induced podosome disassembly. Thus, the actin cytoskeleton of podosomes involved in dendritic cell extracellular matrix degradation appears to be regulated differently than the cytoskeleton in podosomes of osteoclasts mediating bone resorption. |
| first_indexed | 2024-12-14T23:28:11Z |
| format | Article |
| id | doaj.art-990cd1672b7d4f18ab0170464c3c1b4b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T23:28:11Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-990cd1672b7d4f18ab0170464c3c1b4b2022-12-21T22:43:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2161510.1371/journal.pone.0021615Gelsolin-independent podosome formation in dendritic cells.Oscar HammarfjordHervé FaletChristine GurniakJohn H HartwigRobert P A WallinPodosomes, important structures for adhesion and extracellular matrix degradation, are claimed to be involved in cell migration. In addition, podosomes are also reported to be of importance in tissue remodelling, e.g., in osteoclast-mediated bone resorption. Podosomes are highly dynamic actin-filament scaffolds onto which proteins important for their function, such as matrix metallo-proteases and integrins, attach. The dynamics of the podosomes require the action of many proteins regulating actin assembly and disassembly. One such protein, gelsolin, which associates to podosomes, has been reported to be important for podosome formation and function in osteoclasts. However, podosome-like structures have been reported in gelsolin-deficient dendritic cells, but the identity of these structures was not confirmed, and their dynamics and function was not investigated. Like many other cells, dendritic cells of the immune system also form matrix degrading podosomes. In the present study, we show that dendritic cells form podosomes independently of gelsolin, that there are no major alterations in their dynamics of formation and disassembly, and that they exhibit matrix-degrading function. Furthermore, we found that gelsolin is not required for TLR4-induced podosome disassembly. Thus, the actin cytoskeleton of podosomes involved in dendritic cell extracellular matrix degradation appears to be regulated differently than the cytoskeleton in podosomes of osteoclasts mediating bone resorption.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779330/?tool=EBI |
| spellingShingle | Oscar Hammarfjord Hervé Falet Christine Gurniak John H Hartwig Robert P A Wallin Gelsolin-independent podosome formation in dendritic cells. PLoS ONE |
| title | Gelsolin-independent podosome formation in dendritic cells. |
| title_full | Gelsolin-independent podosome formation in dendritic cells. |
| title_fullStr | Gelsolin-independent podosome formation in dendritic cells. |
| title_full_unstemmed | Gelsolin-independent podosome formation in dendritic cells. |
| title_short | Gelsolin-independent podosome formation in dendritic cells. |
| title_sort | gelsolin independent podosome formation in dendritic cells |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779330/?tool=EBI |
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