Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i>
Fungi dwelling in soils contaminated by petroleum products can survive on these hydrocarbons due to the highly effective extracellular enzymes. Species belonging to the genus, Aspergillus are known to be efficient degraders of various classes of hydrocarbons as well as other organic contaminants. I...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2020-06-01
|
| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/196950 |
| _version_ | 1797228111900704768 |
|---|---|
| author | U.C. Odili F.B. Ibrahim E.M. Shaibu-Imodagbe H.I. Atta |
| author_facet | U.C. Odili F.B. Ibrahim E.M. Shaibu-Imodagbe H.I. Atta |
| author_sort | U.C. Odili |
| collection | DOAJ |
| description |
Fungi dwelling in soils contaminated by petroleum products can survive on these hydrocarbons due to the highly effective extracellular enzymes. Species belonging to the genus, Aspergillus are known to be efficient degraders of various classes of hydrocarbons as well as other organic contaminants. In this study, the biodegradation of crude oil by Aspergillus niger and Monocillium sp. were compared using laboratory microcosms.
The moulds were isolated from a site receiving effluent from a petroleum refinery. They were identified using their macroscopic and microscopic characteristics and subsequently screened for their ability to utilize hydrocarbons for their metabolic requirements. Following the biodegradation studies, Aspergillus niger and Monocillium sp. recorded an increase in hydrocarbon utilizing fungal counts of 8.5 x 107 spores/ml and 6.1 x 107 spores/ml and crude oil weight loss of 80 % and 70 %. Both fungi were tested singly and in a consortium for their ability to degrade crude oil, it
was observed that Monocillium sp. and A.niger performed better when tested individually (94.2 %; 92.8 %) than in consortium (76.3 %). This may suggest that their combined metabolism may have created some antagonistic effect on the degradation process as opposed to their enzymatic capabilities which appeared to be more favourable to the process. The biodegradation experiment analysis showed that contact time plays a significant role in biodegradation of crude oil (p<0.05), and Monocillium sp. and Aspergillus niger are excellent crude oil degraders and can be used
in the bioremediation of petroleum-contaminated soil and water.
Keywords: Biodegradation, Aspergillus niger, Monocillium sp., soil, Crude oil
|
| first_indexed | 2024-04-24T14:51:31Z |
| format | Article |
| id | doaj.art-990d01f36790440cb6d4519c28647845 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:51:31Z |
| publishDate | 2020-06-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-990d01f36790440cb6d4519c286478452024-04-02T19:49:22ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992020-06-0124510.4314/jasem.v24i5.13Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i>U.C. OdiliF.B. IbrahimE.M. Shaibu-ImodagbeH.I. Atta Fungi dwelling in soils contaminated by petroleum products can survive on these hydrocarbons due to the highly effective extracellular enzymes. Species belonging to the genus, Aspergillus are known to be efficient degraders of various classes of hydrocarbons as well as other organic contaminants. In this study, the biodegradation of crude oil by Aspergillus niger and Monocillium sp. were compared using laboratory microcosms. The moulds were isolated from a site receiving effluent from a petroleum refinery. They were identified using their macroscopic and microscopic characteristics and subsequently screened for their ability to utilize hydrocarbons for their metabolic requirements. Following the biodegradation studies, Aspergillus niger and Monocillium sp. recorded an increase in hydrocarbon utilizing fungal counts of 8.5 x 107 spores/ml and 6.1 x 107 spores/ml and crude oil weight loss of 80 % and 70 %. Both fungi were tested singly and in a consortium for their ability to degrade crude oil, it was observed that Monocillium sp. and A.niger performed better when tested individually (94.2 %; 92.8 %) than in consortium (76.3 %). This may suggest that their combined metabolism may have created some antagonistic effect on the degradation process as opposed to their enzymatic capabilities which appeared to be more favourable to the process. The biodegradation experiment analysis showed that contact time plays a significant role in biodegradation of crude oil (p<0.05), and Monocillium sp. and Aspergillus niger are excellent crude oil degraders and can be used in the bioremediation of petroleum-contaminated soil and water. Keywords: Biodegradation, Aspergillus niger, Monocillium sp., soil, Crude oil https://www.ajol.info/index.php/jasem/article/view/196950Biodegradation, Aspergillus niger, Monocillium sp., soil, Crude oil |
| spellingShingle | U.C. Odili F.B. Ibrahim E.M. Shaibu-Imodagbe H.I. Atta Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i> Journal of Applied Sciences and Environmental Management Biodegradation, Aspergillus niger, Monocillium sp., soil, Crude oil |
| title | Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i> |
| title_full | Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i> |
| title_fullStr | Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i> |
| title_full_unstemmed | Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i> |
| title_short | Comparative Assessment of Crude Oil Degradation by <i>Monocillium sp.</i> and <i>Aspergillus niger</i> |
| title_sort | comparative assessment of crude oil degradation by i monocillium sp i and i aspergillus niger i |
| topic | Biodegradation, Aspergillus niger, Monocillium sp., soil, Crude oil |
| url | https://www.ajol.info/index.php/jasem/article/view/196950 |
| work_keys_str_mv | AT ucodili comparativeassessmentofcrudeoildegradationbyimonocilliumspiandiaspergillusnigeri AT fbibrahim comparativeassessmentofcrudeoildegradationbyimonocilliumspiandiaspergillusnigeri AT emshaibuimodagbe comparativeassessmentofcrudeoildegradationbyimonocilliumspiandiaspergillusnigeri AT hiatta comparativeassessmentofcrudeoildegradationbyimonocilliumspiandiaspergillusnigeri |