The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen
Background & Objective: Dendritic cell (DC) is as a key cell in activation of immune response against microbes and disease. Therefore, the effect of recombinant exotoxin A of Pseudomonas aeruginosa on the maturity and the activation of DCs was evaluated in this study. Materials & Methods: Re...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2014-12-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-508-en.pdf |
| _version_ | 1797679639177461760 |
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| author | Mohammad Hossein Karimi Aziz Japoni Manouchehr Rasouli Salimeh Ebrahimnezhad |
| author_facet | Mohammad Hossein Karimi Aziz Japoni Manouchehr Rasouli Salimeh Ebrahimnezhad |
| author_sort | Mohammad Hossein Karimi |
| collection | DOAJ |
| description | Background & Objective:
Dendritic cell (DC) is as a key cell in activation of immune response against
microbes and disease. Therefore, the effect of recombinant exotoxin A of
Pseudomonas aeruginosa on the maturity and the activation of DCs was evaluated
in this study. Materials & Methods:
Recombinant exotoxin A was produced from Pseudomonas aeruginosa DNA. MTT assay
was used to evaluate the cytotoxicity of this protein on DCs. The expression of
co-stimulatory molecules CD40, CD86, and MHCΠ was evaluated by flow cytometry.
Moreover, the effect of this antigen (Ag) on T-cell proliferation was evaluated
using Mixed Lymphocyte Reaction (MLR) assay and the secretion of IL-4 and IFN-
γ. Secretion of IL-12 by DCs was measured with Enzyme-Linked
Immunosorbent Assay (ELISA)
method. The data were collected and analyzed with one way ANOVA test. Results:
Recombinant exotoxin A had no effect on DCs viability. In addition, expression
of CD40, CD86, and MHCΠ did not change significantly compared to the negative
control cells. Moreover, T-cells proliferation was decreased significantly at
the concentration of 0.1µg/ml of this Ag. The secretion of IL-12 was increased
by DCs, in contrast the secretion of IL-4 and IFN-γ in MLR supernatant did not
decrease significantly. Conclusion:
Exotoxin A decreases the proliferation of T-cells and also leads to a change in
the pattern of cytokine secretion of immune cells. |
| first_indexed | 2024-03-11T23:17:43Z |
| format | Article |
| id | doaj.art-990da5b1fb034b6f88f224656782b61a |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:43Z |
| publishDate | 2014-12-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-990da5b1fb034b6f88f224656782b61a2023-09-20T21:17:53ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232014-12-0144436445The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice SpleenMohammad Hossein Karimi0Aziz Japoni1Manouchehr Rasouli2Salimeh Ebrahimnezhad3 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Immunology, Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz, Iran. Department of Immunology, Professor Alborzi Clinical Microbiology Research Center, Namazi Hospital, Shiraz, Iran. Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Background & Objective: Dendritic cell (DC) is as a key cell in activation of immune response against microbes and disease. Therefore, the effect of recombinant exotoxin A of Pseudomonas aeruginosa on the maturity and the activation of DCs was evaluated in this study. Materials & Methods: Recombinant exotoxin A was produced from Pseudomonas aeruginosa DNA. MTT assay was used to evaluate the cytotoxicity of this protein on DCs. The expression of co-stimulatory molecules CD40, CD86, and MHCΠ was evaluated by flow cytometry. Moreover, the effect of this antigen (Ag) on T-cell proliferation was evaluated using Mixed Lymphocyte Reaction (MLR) assay and the secretion of IL-4 and IFN- γ. Secretion of IL-12 by DCs was measured with Enzyme-Linked Immunosorbent Assay (ELISA) method. The data were collected and analyzed with one way ANOVA test. Results: Recombinant exotoxin A had no effect on DCs viability. In addition, expression of CD40, CD86, and MHCΠ did not change significantly compared to the negative control cells. Moreover, T-cells proliferation was decreased significantly at the concentration of 0.1µg/ml of this Ag. The secretion of IL-12 was increased by DCs, in contrast the secretion of IL-4 and IFN-γ in MLR supernatant did not decrease significantly. Conclusion: Exotoxin A decreases the proliferation of T-cells and also leads to a change in the pattern of cytokine secretion of immune cells.http://jabs.fums.ac.ir/article-1-508-en.pdfexotoxin adendritic cellimmunomodulatory effects |
| spellingShingle | Mohammad Hossein Karimi Aziz Japoni Manouchehr Rasouli Salimeh Ebrahimnezhad The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen Journal of Advanced Biomedical Sciences exotoxin a dendritic cell immunomodulatory effects |
| title | The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen |
| title_full | The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen |
| title_fullStr | The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen |
| title_full_unstemmed | The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen |
| title_short | The Immunomodulatory Effect of Recombinant Exotoxin A of Pseudomonas Aeruginosa on Dendritic Cells Extracted from Mice Spleen |
| title_sort | immunomodulatory effect of recombinant exotoxin a of pseudomonas aeruginosa on dendritic cells extracted from mice spleen |
| topic | exotoxin a dendritic cell immunomodulatory effects |
| url | http://jabs.fums.ac.ir/article-1-508-en.pdf |
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