SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS
A total of 75 pyogenic samples were collected from patients examinated for pyogenic infection in Sednawy hospital and Elmokhtaber laboratories. S. aureus isolated from pleural fluid out of five S. aureus (+ve coagulase, MSSA) exhibits resistant against all the concentration of Ciprofloxacin 0.5-20,...
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2017-09-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_13621_427367fbb236ab54eaec5d7bfdd5f0a0.pdf |
| _version_ | 1797269749784117248 |
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| author | Asmaa Hussein Rawia Gamal A. Refaat A. Abdel-Salam K. Ramadan |
| author_facet | Asmaa Hussein Rawia Gamal A. Refaat A. Abdel-Salam K. Ramadan |
| author_sort | Asmaa Hussein |
| collection | DOAJ |
| description | A total of 75 pyogenic samples were collected from patients examinated for pyogenic infection in Sednawy hospital and Elmokhtaber laboratories. S. aureus isolated from pleural fluid out of five S. aureus (+ve coagulase, MSSA) exhibits resistant against all the concentration of Ciprofloxacin 0.5-20, Gentamycin 0.5- 5.0 µg/100µl and Amikacin 1 µg/100µl. The highest potent of phytoextracts either extracted by water or methanol was detected by clove comparing with mint, thyme, sage and garlic, whereas garlic essential oil gave completely abolish of S. aureus (P.F). The highest synergism was obtained in combination between the lowest inhibitory concentration of Gentamycin 10 µg/100µl or Vancomycin 1 µg/100µl or Amikacin 2.5 µg/100µl with thyme Eso (1:3), which resulted to increase the efficacy by 4.4 or 1.5 or 1.6 respectively comparing to recommended dose of the tested antibiotics alone. Increasing the concentration of garlic essential oil (GEso) from 10 up to 100 µl/well increment the efficacy of inhibition up to 10, 3.7 and 4.5- fold when standard dose of Gentamycin, Vancomycin and Amikacin were used individually against S. aureus P.F. The minimum bactericidal concentration of garlic essential oil was recorded at 2 µl/ml as it resulted to reduce the count to be 0.04%. Analysis of garlic essential oil by GC-MS dedicated six sulfur compounds represented 88.8644% of total detected compounds in garlic essential oil. |
| first_indexed | 2024-04-25T01:53:20Z |
| format | Article |
| id | doaj.art-9910d776d0ae42b99c9048aff985542b |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:53:20Z |
| publishDate | 2017-09-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-9910d776d0ae42b99c9048aff985542b2024-03-07T17:42:36ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852017-09-0125238740110.21608/ajs.2017.1362113621SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDSAsmaa Hussein0Rawia Gamal1A. Refaat2A. Abdel-Salam3K. Ramadan4Agricultural Microbiology Dept., Faculty of Agriculture, Ain Shams University, Cairo, EgyptAgricultural Microbiology Dept., Faculty of Agriculture, Ain Shams University, Cairo, EgyptAgricultural Microbiology Dept., Faculty of Agriculture, Ain Shams University, Cairo, EgyptBacterial Contamination Dept., ARC, Giza, EgyptBiochemistry Dept., Faculty of Agriculture, Ain Shams University, Cairo, EgyptA total of 75 pyogenic samples were collected from patients examinated for pyogenic infection in Sednawy hospital and Elmokhtaber laboratories. S. aureus isolated from pleural fluid out of five S. aureus (+ve coagulase, MSSA) exhibits resistant against all the concentration of Ciprofloxacin 0.5-20, Gentamycin 0.5- 5.0 µg/100µl and Amikacin 1 µg/100µl. The highest potent of phytoextracts either extracted by water or methanol was detected by clove comparing with mint, thyme, sage and garlic, whereas garlic essential oil gave completely abolish of S. aureus (P.F). The highest synergism was obtained in combination between the lowest inhibitory concentration of Gentamycin 10 µg/100µl or Vancomycin 1 µg/100µl or Amikacin 2.5 µg/100µl with thyme Eso (1:3), which resulted to increase the efficacy by 4.4 or 1.5 or 1.6 respectively comparing to recommended dose of the tested antibiotics alone. Increasing the concentration of garlic essential oil (GEso) from 10 up to 100 µl/well increment the efficacy of inhibition up to 10, 3.7 and 4.5- fold when standard dose of Gentamycin, Vancomycin and Amikacin were used individually against S. aureus P.F. The minimum bactericidal concentration of garlic essential oil was recorded at 2 µl/ml as it resulted to reduce the count to be 0.04%. Analysis of garlic essential oil by GC-MS dedicated six sulfur compounds represented 88.8644% of total detected compounds in garlic essential oil.https://ajs.journals.ekb.eg/article_13621_427367fbb236ab54eaec5d7bfdd5f0a0.pdfs.aureusantibioticsphytoextractsynergistic effectsmbc16s rrnagc-ms spectrometry |
| spellingShingle | Asmaa Hussein Rawia Gamal A. Refaat A. Abdel-Salam K. Ramadan SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS Arab Universities Journal of Agricultural Sciences s.aureus antibiotics phytoextract synergistic effects mbc 16s rrna gc-ms spectrometry |
| title | SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS |
| title_full | SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS |
| title_fullStr | SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS |
| title_full_unstemmed | SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS |
| title_short | SYNERGISTIC EFFECT OF SOME PLANT EXTRACTS AND ANTIBIOTIC DRUGS AGAINST STAPH. AUREUS ISOLATED FROM PLEURAL FLUID IDENTIFICATION OF THE ACTIVE COMPOUNDS |
| title_sort | synergistic effect of some plant extracts and antibiotic drugs against staph aureus isolated from pleural fluid identification of the active compounds |
| topic | s.aureus antibiotics phytoextract synergistic effects mbc 16s rrna gc-ms spectrometry |
| url | https://ajs.journals.ekb.eg/article_13621_427367fbb236ab54eaec5d7bfdd5f0a0.pdf |
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