Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis
Karnal bunt of wheat is an internationally quarantined disease. One of the major constraints in exporting wheat is prevalence of Karnal bunt (KB). It is caused by Tilletia indica which is a heterothallic fungus and requires fusion of different mating types for causing infection. The growth efficienc...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-06-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/90828 |
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| author | JAGMOHAN SINGH RASHMI AGGARWAL MALKHAN SINGH GURJAR SAPNA SHARMA M S SAHARAN |
| author_facet | JAGMOHAN SINGH RASHMI AGGARWAL MALKHAN SINGH GURJAR SAPNA SHARMA M S SAHARAN |
| author_sort | JAGMOHAN SINGH |
| collection | DOAJ |
| description | Karnal bunt of wheat is an internationally quarantined disease. One of the major constraints in exporting wheat is prevalence of Karnal bunt (KB). It is caused by Tilletia indica which is a heterothallic fungus and requires fusion of different mating types for causing infection. The growth efficiency and aggressiveness of plant pathogens are often associated with their carbohydrate active enzymes (CAZymes). The present study was undertaken to identify the CAZymes in whole genome sequence of Tilletia indica, and conduct sporulation analysis. Total 315 predicted secretory proteins were annotated with the CAZyme database (dbCAN). The secreted carbohydrates active enzymes were identified as 105 glycosylhydrolases (GH), 85 glycosyltransferases (GT), 83 carbohydrate esterases (CE), 8 carbohydrate binding modules (CBM), 30 auxiliary activities (AA) and 4 polysaccharide lyases (PL). Based on sporulation analysis of T. indica isolates, KB1 and KB2 isolates were most sporulative among all the isolates of T. indica. These carbohydrate active enzymes can help to understand the pathogenesis mechanism(s) of Karnal bunt using functional genomics approach. |
| first_indexed | 2024-04-10T07:51:08Z |
| format | Article |
| id | doaj.art-99147ca3d028466395b49c39b54c3662 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T07:51:08Z |
| publishDate | 2019-06-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-99147ca3d028466395b49c39b54c36622023-02-23T10:14:21ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192019-06-0189610.56093/ijas.v89i6.90828Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysisJAGMOHAN SINGH0RASHMI AGGARWAL1MALKHAN SINGH GURJAR2SAPNA SHARMA3M S SAHARAN4Ph D Scholar, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaHead, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaScientist SS, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaSRF, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaPrincipal scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012, IndiaKarnal bunt of wheat is an internationally quarantined disease. One of the major constraints in exporting wheat is prevalence of Karnal bunt (KB). It is caused by Tilletia indica which is a heterothallic fungus and requires fusion of different mating types for causing infection. The growth efficiency and aggressiveness of plant pathogens are often associated with their carbohydrate active enzymes (CAZymes). The present study was undertaken to identify the CAZymes in whole genome sequence of Tilletia indica, and conduct sporulation analysis. Total 315 predicted secretory proteins were annotated with the CAZyme database (dbCAN). The secreted carbohydrates active enzymes were identified as 105 glycosylhydrolases (GH), 85 glycosyltransferases (GT), 83 carbohydrate esterases (CE), 8 carbohydrate binding modules (CBM), 30 auxiliary activities (AA) and 4 polysaccharide lyases (PL). Based on sporulation analysis of T. indica isolates, KB1 and KB2 isolates were most sporulative among all the isolates of T. indica. These carbohydrate active enzymes can help to understand the pathogenesis mechanism(s) of Karnal bunt using functional genomics approach.https://epubs.icar.org.in/index.php/IJAgS/article/view/90828Carbohydrate active enzymesKarnal buntSporulationTilletia indicaWheat |
| spellingShingle | JAGMOHAN SINGH RASHMI AGGARWAL MALKHAN SINGH GURJAR SAPNA SHARMA M S SAHARAN Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis The Indian Journal of Agricultural Sciences Carbohydrate active enzymes Karnal bunt Sporulation Tilletia indica Wheat |
| title | Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis |
| title_full | Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis |
| title_fullStr | Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis |
| title_full_unstemmed | Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis |
| title_short | Identification of carbohydrate active enzymes from whole genome sequence of Tilletia indica and sporulation analysis |
| title_sort | identification of carbohydrate active enzymes from whole genome sequence of tilletia indica and sporulation analysis |
| topic | Carbohydrate active enzymes Karnal bunt Sporulation Tilletia indica Wheat |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/90828 |
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