Impact of enzymatic digestion on bacterial community composition in CF airway samples

Impact of enzymatic digestion on bacterial community composition in CF airway samples

Background Previous studies have demonstrated the importance of DNA extraction methods for molecular detection of Staphylococcus, an important bacterial group in cystic fibrosis (CF). We sought to evaluate the effect of enzymatic digestion (EnzD) prior to DNA extraction on bacterial communities iden...

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Bibliographic Details
Main Authors: Kayla M. Williamson, Brandie D. Wagner, Charles E. Robertson, Emily J. Johnson, Edith T. Zemanick, J. Kirk Harris
Format: Article
Language:English
Published: PeerJ Inc. 2017-05-01
Series:PeerJ
Subjects:
16s Sequencing
Microbiome
Sputum
Gram positive
Beta diversity
DNA extraction
Online Access:https://peerj.com/articles/3362.pdf

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https://peerj.com/articles/3362.pdf

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