Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.)
Glufosinate is a broad-spectrum herbicide used to control most weeds in agriculture worldwide. Goosegrass (<i>Eleusine indica</i> L.) is one of the top ten malignant weeds across the world, showing high tolerance to glufosinate via different mechanisms that are not yet fully understood....
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13791 |
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| author | Qiyu Luo Hao Fu Fang Hu Shiguo Li Qiqi Chen Shangming Peng Cunyi Yang Yaoguang Liu Yong Chen |
| author_facet | Qiyu Luo Hao Fu Fang Hu Shiguo Li Qiqi Chen Shangming Peng Cunyi Yang Yaoguang Liu Yong Chen |
| author_sort | Qiyu Luo |
| collection | DOAJ |
| description | Glufosinate is a broad-spectrum herbicide used to control most weeds in agriculture worldwide. Goosegrass (<i>Eleusine indica</i> L.) is one of the top ten malignant weeds across the world, showing high tolerance to glufosinate via different mechanisms that are not yet fully understood. This study revealed that nitrogen metabolism could be a target-resistant site, providing clues to finally clarify the mechanism of glufosinate resistance in resistant goosegrass populations. Compared to susceptible goosegrass (NX), the resistant goosegrass (AUS and CS) regarding the stress of glufosinate showed stronger resistance with lower ammonia contents, higher target enzyme GS (glutamine synthetase) activity, and lower GOGAT (glutamine 2-oxoglutarate aminotransferase) activity. The GDH (glutamate dehydrogenase) activity of another pathway increased, but its gene expression was downregulated in resistant goosegrass (AUS). Analyzing the transcriptome and proteome data of goosegrass under glufosinate stress at 36 h showed that the KEGG pathway of the nitrogen metabolism was enriched in glufosinate-susceptible goosegrass (NX), but not in glufosinate-resistant goosegrass (CS and AUS). Several putative target genes involved in glufosinate stress countermeasures were identified. This study provides specific insights into the nitrogen metabolism of resistant goosegrass, and gives a basis for future functional verification of glufosinate-tolerance genes in plants. |
| first_indexed | 2024-03-10T22:41:24Z |
| format | Article |
| id | doaj.art-991c23ff03604d2aaa29cd13287c14a7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:41:24Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-991c23ff03604d2aaa29cd13287c14a72023-11-19T11:03:11ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181379110.3390/ijms241813791Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.)Qiyu Luo0Hao Fu1Fang Hu2Shiguo Li3Qiqi Chen4Shangming Peng5Cunyi Yang6Yaoguang Liu7Yong Chen8College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Life Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Life Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Life Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaGlufosinate is a broad-spectrum herbicide used to control most weeds in agriculture worldwide. Goosegrass (<i>Eleusine indica</i> L.) is one of the top ten malignant weeds across the world, showing high tolerance to glufosinate via different mechanisms that are not yet fully understood. This study revealed that nitrogen metabolism could be a target-resistant site, providing clues to finally clarify the mechanism of glufosinate resistance in resistant goosegrass populations. Compared to susceptible goosegrass (NX), the resistant goosegrass (AUS and CS) regarding the stress of glufosinate showed stronger resistance with lower ammonia contents, higher target enzyme GS (glutamine synthetase) activity, and lower GOGAT (glutamine 2-oxoglutarate aminotransferase) activity. The GDH (glutamate dehydrogenase) activity of another pathway increased, but its gene expression was downregulated in resistant goosegrass (AUS). Analyzing the transcriptome and proteome data of goosegrass under glufosinate stress at 36 h showed that the KEGG pathway of the nitrogen metabolism was enriched in glufosinate-susceptible goosegrass (NX), but not in glufosinate-resistant goosegrass (CS and AUS). Several putative target genes involved in glufosinate stress countermeasures were identified. This study provides specific insights into the nitrogen metabolism of resistant goosegrass, and gives a basis for future functional verification of glufosinate-tolerance genes in plants.https://www.mdpi.com/1422-0067/24/18/13791nitrogen metabolismherbicide stressglufosinate resistancegoosegrass |
| spellingShingle | Qiyu Luo Hao Fu Fang Hu Shiguo Li Qiqi Chen Shangming Peng Cunyi Yang Yaoguang Liu Yong Chen Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.) International Journal of Molecular Sciences nitrogen metabolism herbicide stress glufosinate resistance goosegrass |
| title | Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.) |
| title_full | Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.) |
| title_fullStr | Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.) |
| title_full_unstemmed | Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.) |
| title_short | Effects of Biological Nitrogen Metabolism on Glufosinate-Susceptible and -Resistant Goosegrass (<i>Eleusine indica</i> L.) |
| title_sort | effects of biological nitrogen metabolism on glufosinate susceptible and resistant goosegrass i eleusine indica i l |
| topic | nitrogen metabolism herbicide stress glufosinate resistance goosegrass |
| url | https://www.mdpi.com/1422-0067/24/18/13791 |
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