Altered sugar donor specificity and catalytic activity of pteridine glycosyltransferases by domain swapping or site-directed mutagenesis

Altered sugar donor specificity and catalytic activity of pteridine glycosyltransferases by domain swapping or site-directed mutagenesis

CY-007 and CY-049 pteridine glycosyltransferases (PGTs) thatdiffer in sugar donor specificity to catalyze either glucose orxylose transfer to tetrahydrobiopterin were studied here touncover the structural determinants necessary for the specificity.The importance of the C-terminal domain and its resi...

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Bibliographic Details
Main Authors: Hye-Lim Kim, Ae Hyun Kim, Mi Bi Park, Soo-Woong Lee, Young Shik Park
Format: Article
Language:English
Published: Korean Society for Biochemistry and Molecular Biology 2013-01-01
Series:BMB Reports
Subjects:
Domain swapping
Pteridine glycosyltransferase
Site-directed mutagenesis
Substrate specificity
Tetrahydrobiopterin
Online Access:http://bmbreports.org/jbmb/pdf.php?data=MTMwOTI2MTZAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0Ni0xJTVEMTMwMTI5MjExNF8lMjgwMzctMDQwJTI5Qk1CXzEyLTE0Ny5wZGY=

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