Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome Coronavirus
Coronaviruses as possible cross-species viruses have caused several epidemics. The ongoing emergency of coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has posed severe threats to the global economy and public health, which has generated great concerns about zoonotic viruses. Swine acute di...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11250 |
| _version_ | 1797479127814504448 |
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| author | Yangzhen Chen Yecheng You Shuqi Wang Lin Jiang Lili Tian Shaozhou Zhu Xiaoping An Lihua Song Yigang Tong Huahao Fan |
| author_facet | Yangzhen Chen Yecheng You Shuqi Wang Lin Jiang Lili Tian Shaozhou Zhu Xiaoping An Lihua Song Yigang Tong Huahao Fan |
| author_sort | Yangzhen Chen |
| collection | DOAJ |
| description | Coronaviruses as possible cross-species viruses have caused several epidemics. The ongoing emergency of coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has posed severe threats to the global economy and public health, which has generated great concerns about zoonotic viruses. Swine acute diarrhea syndrome coronavirus (SADS-CoV), an alpha-coronavirus, was responsible for mass piglet deaths, resulting in unprecedented economic losses, and no approved drugs or vaccines are currently available for SADS-CoV infection. Given its potential ability to cause cross-species infection, it is essential to develop specific antiviral drugs and vaccines against SADS-CoV. Drug screening was performed on a total of 3523 compound-containing drug libraries as a strategy of existing medications repurposing. We identified five compounds (gemcitabine, mycophenolate mofetil, mycophenolic acid, methylene blue and cepharanthine) exhibiting inhibitory effects against SADS-CoV in a dose-dependent manner. Cepharanthine and methylene blue were confirmed to block viral entry, and gemcitabine, mycophenolate mofetil, mycophenolic acid and methylene blue could inhibit viral replication after SADS-CoV entry. This is the first report on SADS-CoV drug screening, and we found five compounds from drug libraries to be potential anti-SADS-CoV drugs, supporting the development of antiviral drugs for a possible outbreak of SADS-CoV in the future. |
| first_indexed | 2024-03-09T21:41:37Z |
| format | Article |
| id | doaj.art-991e5bd600b949b7ae07fec6369b9642 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:41:37Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-991e5bd600b949b7ae07fec6369b96422023-11-23T20:29:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191125010.3390/ijms231911250Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome CoronavirusYangzhen Chen0Yecheng You1Shuqi Wang2Lin Jiang3Lili Tian4Shaozhou Zhu5Xiaoping An6Lihua Song7Yigang Tong8Huahao Fan9College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, ChinaCoronaviruses as possible cross-species viruses have caused several epidemics. The ongoing emergency of coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has posed severe threats to the global economy and public health, which has generated great concerns about zoonotic viruses. Swine acute diarrhea syndrome coronavirus (SADS-CoV), an alpha-coronavirus, was responsible for mass piglet deaths, resulting in unprecedented economic losses, and no approved drugs or vaccines are currently available for SADS-CoV infection. Given its potential ability to cause cross-species infection, it is essential to develop specific antiviral drugs and vaccines against SADS-CoV. Drug screening was performed on a total of 3523 compound-containing drug libraries as a strategy of existing medications repurposing. We identified five compounds (gemcitabine, mycophenolate mofetil, mycophenolic acid, methylene blue and cepharanthine) exhibiting inhibitory effects against SADS-CoV in a dose-dependent manner. Cepharanthine and methylene blue were confirmed to block viral entry, and gemcitabine, mycophenolate mofetil, mycophenolic acid and methylene blue could inhibit viral replication after SADS-CoV entry. This is the first report on SADS-CoV drug screening, and we found five compounds from drug libraries to be potential anti-SADS-CoV drugs, supporting the development of antiviral drugs for a possible outbreak of SADS-CoV in the future.https://www.mdpi.com/1422-0067/23/19/11250antiviral drugscoronavirusSADS-CoVdrug screening |
| spellingShingle | Yangzhen Chen Yecheng You Shuqi Wang Lin Jiang Lili Tian Shaozhou Zhu Xiaoping An Lihua Song Yigang Tong Huahao Fan Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome Coronavirus International Journal of Molecular Sciences antiviral drugs coronavirus SADS-CoV drug screening |
| title | Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome Coronavirus |
| title_full | Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome Coronavirus |
| title_fullStr | Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome Coronavirus |
| title_full_unstemmed | Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome Coronavirus |
| title_short | Antiviral Drugs Screening for Swine Acute Diarrhea Syndrome Coronavirus |
| title_sort | antiviral drugs screening for swine acute diarrhea syndrome coronavirus |
| topic | antiviral drugs coronavirus SADS-CoV drug screening |
| url | https://www.mdpi.com/1422-0067/23/19/11250 |
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