Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community
Abstract Background In agricultural production, fungal diseases significantly impact the yield and quality of cotton (Gossypium spp.) with Verticillium wilt posing a particularly severe threat. Results This study is focused on investigating the effectiveness of endophytic microbial communities prese...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-04910-2 |
| _version_ | 1797209425554964480 |
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| author | Chong-Die Wu Yong-Bin Fan Xue Chen Jiang-Wei Cao Jing-Yi Ye Meng-Lei Feng Xing-Xing Liu Wen-Jing Sun Rui-Na Liu Ai-Ying Wang |
| author_facet | Chong-Die Wu Yong-Bin Fan Xue Chen Jiang-Wei Cao Jing-Yi Ye Meng-Lei Feng Xing-Xing Liu Wen-Jing Sun Rui-Na Liu Ai-Ying Wang |
| author_sort | Chong-Die Wu |
| collection | DOAJ |
| description | Abstract Background In agricultural production, fungal diseases significantly impact the yield and quality of cotton (Gossypium spp.) with Verticillium wilt posing a particularly severe threat. Results This study is focused on investigating the effectiveness of endophytic microbial communities present in the seeds of disease-resistant cotton genotypes in the control of cotton Verticillium wilt. The technique of 16S ribosomal RNA (16S rRNA) amplicon sequencing identified a significant enrichment of the Bacillus genus in the resistant genotype Xinluzao 78, which differed from the endophytic bacterial community structure in the susceptible genotype Xinluzao 63. Specific enriched strains were isolated and screened from the seeds of Xinluzao 78 to further explore the biological functions of seed endophytes. A synthetic microbial community (SynCom) was constructed using the broken-rod model, and seeds of the susceptible genotype Xinluzao 63 in this community that had been soaked with the SynCom were found to significantly control the occurrence of Verticillium wilt and regulate the growth of cotton plants. Antibiotic screening techniques were used to preliminarily identify the colonization of strains in the community. These techniques revealed that the strains can colonize plant tissues and occupy ecological niches in cotton tissues through a priority effect, which prevents infection by pathogens. Conclusion This study highlights the key role of seed endophytes in driving plant disease defense and provides a theoretical basis for the future application of SynComs in agriculture. |
| first_indexed | 2024-04-24T09:54:30Z |
| format | Article |
| id | doaj.art-99200ebacb704456a3792e1327b14410 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-24T09:54:30Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-99200ebacb704456a3792e1327b144102024-04-14T11:12:02ZengBMCBMC Plant Biology1471-22292024-04-0124111510.1186/s12870-024-04910-2Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial communityChong-Die Wu0Yong-Bin Fan1Xue Chen2Jiang-Wei Cao3Jing-Yi Ye4Meng-Lei Feng5Xing-Xing Liu6Wen-Jing Sun7Rui-Na Liu8Ai-Ying Wang9College of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityAbstract Background In agricultural production, fungal diseases significantly impact the yield and quality of cotton (Gossypium spp.) with Verticillium wilt posing a particularly severe threat. Results This study is focused on investigating the effectiveness of endophytic microbial communities present in the seeds of disease-resistant cotton genotypes in the control of cotton Verticillium wilt. The technique of 16S ribosomal RNA (16S rRNA) amplicon sequencing identified a significant enrichment of the Bacillus genus in the resistant genotype Xinluzao 78, which differed from the endophytic bacterial community structure in the susceptible genotype Xinluzao 63. Specific enriched strains were isolated and screened from the seeds of Xinluzao 78 to further explore the biological functions of seed endophytes. A synthetic microbial community (SynCom) was constructed using the broken-rod model, and seeds of the susceptible genotype Xinluzao 63 in this community that had been soaked with the SynCom were found to significantly control the occurrence of Verticillium wilt and regulate the growth of cotton plants. Antibiotic screening techniques were used to preliminarily identify the colonization of strains in the community. These techniques revealed that the strains can colonize plant tissues and occupy ecological niches in cotton tissues through a priority effect, which prevents infection by pathogens. Conclusion This study highlights the key role of seed endophytes in driving plant disease defense and provides a theoretical basis for the future application of SynComs in agriculture.https://doi.org/10.1186/s12870-024-04910-2High-throughput sequencingSeed microbiomeCotton Verticillium wiltMicrobial community constructionSynthetic Microbial Community (SynCom) |
| spellingShingle | Chong-Die Wu Yong-Bin Fan Xue Chen Jiang-Wei Cao Jing-Yi Ye Meng-Lei Feng Xing-Xing Liu Wen-Jing Sun Rui-Na Liu Ai-Ying Wang Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community BMC Plant Biology High-throughput sequencing Seed microbiome Cotton Verticillium wilt Microbial community construction Synthetic Microbial Community (SynCom) |
| title | Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community |
| title_full | Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community |
| title_fullStr | Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community |
| title_full_unstemmed | Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community |
| title_short | Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community |
| title_sort | analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of verticillium wilt pathogen infection by a synthetic microbial community |
| topic | High-throughput sequencing Seed microbiome Cotton Verticillium wilt Microbial community construction Synthetic Microbial Community (SynCom) |
| url | https://doi.org/10.1186/s12870-024-04910-2 |
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