New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples
Squash mosaic virus (SqMV) is a phytovirus that infects great diversity of plants worldwide. In Brazil, the SqMV has been identified in the states of Ceará, Maranhão, Piauí, Rio Grande do Norte, and Tocantins. The presence of non-pathogenic viruses in animals, such as phytoviruses, may not be comple...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/7/1349 |
| _version_ | 1797529232356671488 |
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| author | Fabiola Villanova Roberta Marcatti Mayara Bertanhe Vanessa dos Santos Morais Flavio Augusto de Padua Milagres Rafael Brustulin Emerson Luiz Lima Araújo Roozbeh Tahmasebi Steven S. Witkin Xutao Deng Eric Delwart Ester Cerdeira Sabino Cassio Hamilton Abreu-Junior Élcio Leal Antonio Charlys da Costa |
| author_facet | Fabiola Villanova Roberta Marcatti Mayara Bertanhe Vanessa dos Santos Morais Flavio Augusto de Padua Milagres Rafael Brustulin Emerson Luiz Lima Araújo Roozbeh Tahmasebi Steven S. Witkin Xutao Deng Eric Delwart Ester Cerdeira Sabino Cassio Hamilton Abreu-Junior Élcio Leal Antonio Charlys da Costa |
| author_sort | Fabiola Villanova |
| collection | DOAJ |
| description | Squash mosaic virus (SqMV) is a phytovirus that infects great diversity of plants worldwide. In Brazil, the SqMV has been identified in the states of Ceará, Maranhão, Piauí, Rio Grande do Norte, and Tocantins. The presence of non-pathogenic viruses in animals, such as phytoviruses, may not be completely risk-free. Similarities in gene repertories between these viruses and viruses that affect animal species have been reported. The present study describes the fully sequenced genomes of SqMV found in human feces, collected in Tocantins, and analyzes the viral profile by metagenomics in the context of diarrhea symptomatology. The complete SqMV genome was obtained in 39 of 253 analyzed samples (15.5%); 97.4% of them belonged to children under 5 years old. There was no evidence that the observed symptoms were related to the presence of SqMV. Of the different virus species detected in these fecal samples, at least 4 (rotavirus, sapovirus, norovirus, parechovirus) are widely known to cause gastrointestinal symptoms. The presence of SqMV nucleic acid in fecal samples is likely due to recent dietary consumption and it is not evidence of viral replication in the human intestinal cells. Identifying the presence of SqMV in human feces and characterization of its genome is a relevant precursor to determining whether and how plant viruses interact with host cells or microorganisms in the human gastrointestinal tract. |
| first_indexed | 2024-03-10T10:10:50Z |
| format | Article |
| id | doaj.art-9920476f88ca43d7a6d6e747fc38bd69 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:10:50Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9920476f88ca43d7a6d6e747fc38bd692023-11-22T01:11:11ZengMDPI AGMicroorganisms2076-26072021-06-0197134910.3390/microorganisms9071349New Variants of Squash Mosaic Viruses Detected in Human Fecal SamplesFabiola Villanova0Roberta Marcatti1Mayara Bertanhe2Vanessa dos Santos Morais3Flavio Augusto de Padua Milagres4Rafael Brustulin5Emerson Luiz Lima Araújo6Roozbeh Tahmasebi7Steven S. Witkin8Xutao Deng9Eric Delwart10Ester Cerdeira Sabino11Cassio Hamilton Abreu-Junior12Élcio Leal13Antonio Charlys da Costa14Laboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, PA, BrazilDepartamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, BrazilDepartamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, BrazilDepartamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, BrazilInstituto de Ciências Biológicas, Universidade Federal do Tocantins, Palmas 77001-090, TO, BrazilInstituto de Ciências Biológicas, Universidade Federal do Tocantins, Palmas 77001-090, TO, BrazilGeneral Coordination of Public Health, Laboratories of the Strategic Articulation, Department of the Health, Surveillance Secretariat, Ministry of Health (CGLAB/DAEVS/SVS-MS), Brasília 70719-040, DF, BrazilDepartamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, BrazilDepartamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, BrazilVitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94143, USAVitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94143, USADepartamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, BrazilCenter of Nuclear Energy in Agriculture, Universidade de São Paulo, Piracicaba 3400-970, SP, BrazilLaboratório de Diversidade Viral, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belem 66075-000, PA, BrazilDepartamento de Moléstias Infecciosas e Parasitárias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, BrazilSquash mosaic virus (SqMV) is a phytovirus that infects great diversity of plants worldwide. In Brazil, the SqMV has been identified in the states of Ceará, Maranhão, Piauí, Rio Grande do Norte, and Tocantins. The presence of non-pathogenic viruses in animals, such as phytoviruses, may not be completely risk-free. Similarities in gene repertories between these viruses and viruses that affect animal species have been reported. The present study describes the fully sequenced genomes of SqMV found in human feces, collected in Tocantins, and analyzes the viral profile by metagenomics in the context of diarrhea symptomatology. The complete SqMV genome was obtained in 39 of 253 analyzed samples (15.5%); 97.4% of them belonged to children under 5 years old. There was no evidence that the observed symptoms were related to the presence of SqMV. Of the different virus species detected in these fecal samples, at least 4 (rotavirus, sapovirus, norovirus, parechovirus) are widely known to cause gastrointestinal symptoms. The presence of SqMV nucleic acid in fecal samples is likely due to recent dietary consumption and it is not evidence of viral replication in the human intestinal cells. Identifying the presence of SqMV in human feces and characterization of its genome is a relevant precursor to determining whether and how plant viruses interact with host cells or microorganisms in the human gastrointestinal tract.https://www.mdpi.com/2076-2607/9/7/1349Squash mosaic virusplant virusesnext generation sequencingviromepublic health |
| spellingShingle | Fabiola Villanova Roberta Marcatti Mayara Bertanhe Vanessa dos Santos Morais Flavio Augusto de Padua Milagres Rafael Brustulin Emerson Luiz Lima Araújo Roozbeh Tahmasebi Steven S. Witkin Xutao Deng Eric Delwart Ester Cerdeira Sabino Cassio Hamilton Abreu-Junior Élcio Leal Antonio Charlys da Costa New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples Microorganisms Squash mosaic virus plant viruses next generation sequencing virome public health |
| title | New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples |
| title_full | New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples |
| title_fullStr | New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples |
| title_full_unstemmed | New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples |
| title_short | New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples |
| title_sort | new variants of squash mosaic viruses detected in human fecal samples |
| topic | Squash mosaic virus plant viruses next generation sequencing virome public health |
| url | https://www.mdpi.com/2076-2607/9/7/1349 |
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