Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension
Fetal undernutrition programs hypertension and cardiovascular diseases, and resistance artery remodeling may be a contributing factor. We aimed to assess if fetal undernutrition induces resistance artery remodeling and the relationship with hypertension. Sprague–Dawley dams were fed ad libitum (Cont...
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MDPI AG
2020-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/10/424 |
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| author | Perla Y. Gutiérrez-Arzapalo Pilar Rodríguez-Rodríguez David Ramiro-Cortijo Marta Gil-Ortega Beatriz Somoza Ángel Luis López de Pablo Maria del Carmen González Silvia M. Arribas |
| author_facet | Perla Y. Gutiérrez-Arzapalo Pilar Rodríguez-Rodríguez David Ramiro-Cortijo Marta Gil-Ortega Beatriz Somoza Ángel Luis López de Pablo Maria del Carmen González Silvia M. Arribas |
| author_sort | Perla Y. Gutiérrez-Arzapalo |
| collection | DOAJ |
| description | Fetal undernutrition programs hypertension and cardiovascular diseases, and resistance artery remodeling may be a contributing factor. We aimed to assess if fetal undernutrition induces resistance artery remodeling and the relationship with hypertension. Sprague–Dawley dams were fed ad libitum (Control) or with 50% of control intake between days 11 and 21 of gestation (maternal undernutrition, MUN). In six-month-old male and female offspring we assessed blood pressure (anesthetized and tail-cuff); mesenteric resistance artery (MRA) structure and mechanics (pressure myography), cellular and internal elastic lamina (IEL) organization (confocal microscopy) and plasma MMP-2 and MMP-9 activity (zymography). Systolic blood pressure (SBP, tail-cuff) and plasma MMP activity were assessed in 18-month-old rats. At the age of six months MUN males exhibited significantly higher blood pressure (anesthetized or tail-cuff) and plasma MMP-9 activity, while MUN females did not exhibit significant differences, compared to sex-matched controls. MRA from 6-month-old MUN males and females showed a smaller diameter, reduced adventitial, smooth muscle cell density and IEL fenestra area, and a leftward shift of stress-strain curves. At the age of eighteen months SBP and MMP-9 activity were higher in both MUN males and females, compared to sex-matched controls. These data suggest that fetal undernutrition induces MRA inward eutrophic remodeling and stiffness in both sexes, independent of blood pressure level. Resistance artery structural and mechanical alterations can participate in the development of hypertension in aged females and may contribute to adverse cardiovascular events associated with low birth weight in both sexes. |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T15:34:43Z |
| publishDate | 2020-10-01 |
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| series | Biomedicines |
| spelling | doaj.art-9920c145b63747d3889680191d483c472023-11-20T17:21:28ZengMDPI AGBiomedicines2227-90592020-10-0181042410.3390/biomedicines8100424Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of HypertensionPerla Y. Gutiérrez-Arzapalo0Pilar Rodríguez-Rodríguez1David Ramiro-Cortijo2Marta Gil-Ortega3Beatriz Somoza4Ángel Luis López de Pablo5Maria del Carmen González6Silvia M. Arribas7Center of Research and Teaching in Health Sciences (CIDOCS), Universidad Autonoma de Sinaloa, Av. Cedros y calle Sauces s/n, Culiacán 80010, Sinaloa, MexicoDepartment of Physiology, Faculty of Medicine, Universidad Autonoma de Madrid, C/Arzobispo Morcillo 2, 28029 Madrid, SpainDepartment of Physiology, Faculty of Medicine, Universidad Autonoma de Madrid, C/Arzobispo Morcillo 2, 28029 Madrid, SpainDepartment of Pharmaceutical and Health Sciences, Faculty of Pharmacy, Universidad CEU-San Pablo, C/Julián Romea, 23, 28003 Madrid, SpainDepartment of Pharmaceutical and Health Sciences, Faculty of Pharmacy, Universidad CEU-San Pablo, C/Julián Romea, 23, 28003 Madrid, SpainDepartment of Physiology, Faculty of Medicine, Universidad Autonoma de Madrid, C/Arzobispo Morcillo 2, 28029 Madrid, SpainDepartment of Physiology, Faculty of Medicine, Universidad Autonoma de Madrid, C/Arzobispo Morcillo 2, 28029 Madrid, SpainDepartment of Physiology, Faculty of Medicine, Universidad Autonoma de Madrid, C/Arzobispo Morcillo 2, 28029 Madrid, SpainFetal undernutrition programs hypertension and cardiovascular diseases, and resistance artery remodeling may be a contributing factor. We aimed to assess if fetal undernutrition induces resistance artery remodeling and the relationship with hypertension. Sprague–Dawley dams were fed ad libitum (Control) or with 50% of control intake between days 11 and 21 of gestation (maternal undernutrition, MUN). In six-month-old male and female offspring we assessed blood pressure (anesthetized and tail-cuff); mesenteric resistance artery (MRA) structure and mechanics (pressure myography), cellular and internal elastic lamina (IEL) organization (confocal microscopy) and plasma MMP-2 and MMP-9 activity (zymography). Systolic blood pressure (SBP, tail-cuff) and plasma MMP activity were assessed in 18-month-old rats. At the age of six months MUN males exhibited significantly higher blood pressure (anesthetized or tail-cuff) and plasma MMP-9 activity, while MUN females did not exhibit significant differences, compared to sex-matched controls. MRA from 6-month-old MUN males and females showed a smaller diameter, reduced adventitial, smooth muscle cell density and IEL fenestra area, and a leftward shift of stress-strain curves. At the age of eighteen months SBP and MMP-9 activity were higher in both MUN males and females, compared to sex-matched controls. These data suggest that fetal undernutrition induces MRA inward eutrophic remodeling and stiffness in both sexes, independent of blood pressure level. Resistance artery structural and mechanical alterations can participate in the development of hypertension in aged females and may contribute to adverse cardiovascular events associated with low birth weight in both sexes.https://www.mdpi.com/2227-9059/8/10/424fetal programminghypertensionresistance arteriesmetalloproteasesvascular mechanicsvascular remodeling |
| spellingShingle | Perla Y. Gutiérrez-Arzapalo Pilar Rodríguez-Rodríguez David Ramiro-Cortijo Marta Gil-Ortega Beatriz Somoza Ángel Luis López de Pablo Maria del Carmen González Silvia M. Arribas Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension Biomedicines fetal programming hypertension resistance arteries metalloproteases vascular mechanics vascular remodeling |
| title | Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension |
| title_full | Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension |
| title_fullStr | Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension |
| title_full_unstemmed | Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension |
| title_short | Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension |
| title_sort | fetal undernutrition induces resistance artery remodeling and stiffness in male and female rats independent of hypertension |
| topic | fetal programming hypertension resistance arteries metalloproteases vascular mechanics vascular remodeling |
| url | https://www.mdpi.com/2227-9059/8/10/424 |
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