Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress
Low temperature is one of the major abiotic stresses that restrict the growth and development of maize seedlings. Membrane lipid metabolism and remodeling are key strategies for plants to cope with temperature stresses. In this study, an integrated lipidomic and transcriptomic analysis was performed...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.639132/full |
| _version_ | 1818726915880517632 |
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| author | Xunchao Zhao Xunchao Zhao Yulei Wei Jinjie Zhang Li Yang Xinyu Liu Haiyang Zhang Wenjing Shao Lin He Zuotong Li Yifei Zhang Jingyu Xu |
| author_facet | Xunchao Zhao Xunchao Zhao Yulei Wei Jinjie Zhang Li Yang Xinyu Liu Haiyang Zhang Wenjing Shao Lin He Zuotong Li Yifei Zhang Jingyu Xu |
| author_sort | Xunchao Zhao |
| collection | DOAJ |
| description | Low temperature is one of the major abiotic stresses that restrict the growth and development of maize seedlings. Membrane lipid metabolism and remodeling are key strategies for plants to cope with temperature stresses. In this study, an integrated lipidomic and transcriptomic analysis was performed to explore the metabolic changes of membrane lipids in the roots of maize seedlings under cold stress (5°C). The results revealed that major extraplastidic phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), and phosphatidylinositol (PI)] were dominant membrane lipids in maize root tissues, accounting for more than 70% of the total lipids. In the transcriptome data of maize roots under cold stress, a total of 189 lipid-related differentially expressed genes (DEGs) were annotated and classified into various lipid metabolism pathways, and most of the DEGs were enriched in the “Eukaryotic phospholipid synthesis” (12%), “Fatty acid elongation” (12%), and “Phospholipid signaling” (13%) pathways. Under low temperature stress, the molar percentage of the most abundant phospholipid PC decreased around 10%. The significantly up-regulated expression of genes encoding phospholipase [phospholipase D (PLD)] and phosphatase PAP/LPP genes implied that PC turnover was triggered by cold stress mainly via the PLD pathway. Consequently, as the central product of PC turnover, the level of PA increased drastically (63.2%) compared with the control. The gene-metabolite network and co-expression network were constructed with the prominent lipid-related DEGs to illustrate the modular regulation of metabolic changes of membrane lipids. This study will help to explicate membrane lipid remodeling and the molecular regulation mechanism in field crops encountering low temperature stress. |
| first_indexed | 2024-12-17T22:05:48Z |
| format | Article |
| id | doaj.art-9928f7b2ed51487e8c81cfeb9494ff35 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T22:05:48Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9928f7b2ed51487e8c81cfeb9494ff352022-12-21T21:30:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-04-011210.3389/fpls.2021.639132639132Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold StressXunchao Zhao0Xunchao Zhao1Yulei Wei2Jinjie Zhang3Li Yang4Xinyu Liu5Haiyang Zhang6Wenjing Shao7Lin He8Zuotong Li9Yifei Zhang10Jingyu Xu11Key Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaCollege of Agriculture, Northeast Agricultural University, Harbin, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Lab of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province, College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, ChinaLow temperature is one of the major abiotic stresses that restrict the growth and development of maize seedlings. Membrane lipid metabolism and remodeling are key strategies for plants to cope with temperature stresses. In this study, an integrated lipidomic and transcriptomic analysis was performed to explore the metabolic changes of membrane lipids in the roots of maize seedlings under cold stress (5°C). The results revealed that major extraplastidic phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), and phosphatidylinositol (PI)] were dominant membrane lipids in maize root tissues, accounting for more than 70% of the total lipids. In the transcriptome data of maize roots under cold stress, a total of 189 lipid-related differentially expressed genes (DEGs) were annotated and classified into various lipid metabolism pathways, and most of the DEGs were enriched in the “Eukaryotic phospholipid synthesis” (12%), “Fatty acid elongation” (12%), and “Phospholipid signaling” (13%) pathways. Under low temperature stress, the molar percentage of the most abundant phospholipid PC decreased around 10%. The significantly up-regulated expression of genes encoding phospholipase [phospholipase D (PLD)] and phosphatase PAP/LPP genes implied that PC turnover was triggered by cold stress mainly via the PLD pathway. Consequently, as the central product of PC turnover, the level of PA increased drastically (63.2%) compared with the control. The gene-metabolite network and co-expression network were constructed with the prominent lipid-related DEGs to illustrate the modular regulation of metabolic changes of membrane lipids. This study will help to explicate membrane lipid remodeling and the molecular regulation mechanism in field crops encountering low temperature stress.https://www.frontiersin.org/articles/10.3389/fpls.2021.639132/fullmaize (Zea mays L.)lipid metabolismlipidometranscriptomecold stress |
| spellingShingle | Xunchao Zhao Xunchao Zhao Yulei Wei Jinjie Zhang Li Yang Xinyu Liu Haiyang Zhang Wenjing Shao Lin He Zuotong Li Yifei Zhang Jingyu Xu Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress Frontiers in Plant Science maize (Zea mays L.) lipid metabolism lipidome transcriptome cold stress |
| title | Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress |
| title_full | Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress |
| title_fullStr | Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress |
| title_full_unstemmed | Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress |
| title_short | Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress |
| title_sort | membrane lipids metabolism and transcriptional regulation in maize roots under cold stress |
| topic | maize (Zea mays L.) lipid metabolism lipidome transcriptome cold stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.639132/full |
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